Nicotiana tabacum L. ÜZERİNE AŞILANMIŞ Lycopersicon esculentum Mill.’in RASTGELE ÇOĞALTILMIŞ POLİMORFİK DNA ANALİZİ İLE GENETİK STABİLİTENİN BELİRLENMESİ

Year 2012, Volume: 16 Issue: 2, 37 - 44, 20.02.2014

Didem Körpe Özlem İşeri Feride Şahin Mehmet Haberal

Abstract

Aşılama, tarım ve ormancılıkta sıkça kullanılan bir yöntemdir. Aşılı bitkiler, kalem ve anaç bitkilerinden farklı fenotipik özellikler gösterebilir. Yapılan çalışmalar, aşılamanın genetik varyasyonu ve kalıtımı etkileyebileceğini belirtmiştir. Genetik belirteç yöntemi olarak Rastgele Çoğaltılmış Polimorfik DNA (Random Amplified Polymorphic DNA Analysis; RAPD) analizinin kullanılması ile genotipik değişimler belirlenebilmektedir. Bu çalışmada amaç, tütün üzerine aşılanmış domates bitkisindeki genetik stabilitenin veya moleküler düzeyde aşı etkili değişimlerin RAPD yöntemi ile tanımlanmasıdır. Aşılama deneylerinde kalem olarak domates bitkisinin (Lycopersicum esculantum Mill.) ticari H2274 çeşidi, anaç olarak ise olan tütün bitkisinin (Nicotiana tabacum L.) Samsun çeşidi kullanılmıştır. Aşılamalar yarma aşılama tekniği ile yapılmış ve sera ortamına aktarılmıştır. Aşılamadan 60 gün sonra aşılı ve aşısız bitkilerin taze yapraklarından DNA izole edilmiştir. Kontrol olarak kullanılan aşısız domates bitkilerinden 8 adet primer ile elde edilen RAPD profillerde toplam 102 adet bant tespit edilmiştir. Aşılı örneklerdeki polimorfizm oranı %2.94 ve genomik kalıp stabilite oranı ise %97.06 olarak hesaplanmıştır. Yüksek genetik stabiliteye sahip aşılama teknikleri, genetik transformasyon olmaksızın ürün eldesini arttırarak tarımsal uygulamaları geliştirebilmektedir. 

Keywords

Domates, Tütün, Kalem, anaç, GKS

DETERMINATION of GENETIC STABILITY of TOMATO (Lycopersicon esculentum Mill.) GRAFTED on TOBACCO (Nicotiana tabacum L.) by RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS

Abstract

Grafting has been widely used in agriculture, forestry and horticulture. Grafted plants may exhibit some phenotypic variations from scions and rootstock plants. Existance and possible mechanisms of graft induced genetic variation and inheritance of graft induced characters has been debated among researchers. Use of random amplified polymorphic DNA (RAPD) as genetic marker assay enables detection of genotypic alterations. The prupose of the present study was assessment of genetic stability or any graftinduced changes at molecular level in tomato grafted on tobacco rootstock by RAPD analysis. Nicotiana tabacum L. cv. Samsun was used as rootstock and a Lycopersicon esculentum Mill. commercial cv. H2274 was used as scion in grafts. Plants were grafted by cleft grafting method and transferred to soil field in greenhouse. DNA was isolated from fresh leaves of grafted and non-grafted plants 60 days after
grafting. RAPD analysis revealed that 8 primers gave a total of 102 bands in the profiles of non-grafted tomato plants. Polymorphism of the grafted tomato plants was caluculated as 2.94% and in concordance, Genomic template stability (GTS) was 97.06%. Succesive grafting with high genomic stability, may improve crop yield in agricultural practices without involvement of genetic transformation.

Keywords

Tomato, Tobacco, Scion, Rootstock, GTS

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