Bazı Kiraz Anaçlarının Genetik Akrabalık İlişkilerinin RAPD Moleküler Markır Yöntemi İle Belirlenmesi

Year 2018, Volume: 22 Issue: 3, 326 - 334, 24.09.2018

Hasan Pınar , Mehmet Yaman , Hasan Cumhur Sarısu , Aydın Uzun , Merve Arefe Yiğit

https://doi.org/10.29050/harranziraat.410847

Cited By: 1

Abstract

Bu
çalışmada kiraza anaç olarak kullanılan ve/veya kullanılacak olan bazı genotiplerin
RAPD moleküler markır yöntemi kullanılarak genetik akrabalıklarının belirlenmesi
amaçlanmıştır. Yirmi farklı kiraz anacının (9 adeti kuş kirazı) genetik benzerliklerinin
belirlemesinde 16 farklı RAPD primerinin kullanıldığı çalışmada, 16 primerden
92’si polimorfik olmak üzere 109 bant elde edilmiş ve ortalama polimorfizm
oranı %83.73 olarak belirlenmiştir. Elde edilen bulgulara göre kiraz anacı genotipleri arasındaki
genetik benzerlik 0.60-0.95 arasında değişmiş olup en
düşük benzerlik % 60 ile SL64 genotipi ile diğer tipler arasında, en yüksek
benzerlik oranı ise %95 ile Kuş Kirazı-3 ve Kuş Kirazı-4 genotiplerinden elde
edilmiştir. Elde edilen dendograma göre üç ana grup oluşmuş olup, birinci grup Kuş
Kirazı-2 (KK2), Kuş Kirazı-6 (KK6), Kuş Kirazı-7 (KK7) ve Kuş Kirazı-8 (KK8)
yer almıştır.  İkinci grupta Kuş Kirazı-3
(KK3), Kuş Kirazı-4 (KK4), Kuş Kirazı-5 (KK5), Kuş Kirazı-9 (KK9), Kuş
Kirazı-10 (KK10), İdris ve diğer yabancı anaçlar yer almıştır. Üçüncü grupta  sadece SL64 genotipi yer almıştır. Elde
edilen bulgular RAPD moleküler markır tekniğinin kiraz anaçları arasındaki
genetik ilişkilerin belirlenmesinde etkin bir şekilde kullanılabileceğini
göstermektedir.

Keywords

Prunus avium L., Kiraz, Anaç, Genetik çeşitlilik, RAPD

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