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The effect on polphenol oxidase activity of chemical and hand fruit thinning applications in some apple cultivars

Year 2019, Volume: 23 Issue: 2, 211 - 217, 18.06.2019

Abstract

In this study, MM 106 clone rootstock grafted on to Golden
Delicious, Granny Smith and Red Chief apple cultivars were applied Naphthalene
Acetic Acid (NAA) (5, 10 ppm) and Benzyladenine (BA) (100, 150 ppm) with hand
and chemical thinning. Apple cultivars collected during harvest according to
applications was determined polyphenol oxidase activity. According to the
results of statistical analysis, the effect of application and variety x
application interaction on polyphenol oxidase activity was found to be
important (P<0.01). According to the results, polyphenol oxidase activity of
Red Chief variety was higher in all applications compared to other varieties.
As the NAA doses increased, while the activity of the polyphenol oxidase enzyme
in the Golden Delicious and Red Chiefs increased, polyphenol oxidase activity
decreased in Granny Smith variety. On the other hand, with the increase in the
dose of BA, polyphenol oxidase activity was decreased in Golden Delicious and
Red Chief, polyphenol oxidase activity increased in Granny Smith variety.

References

  • Aydemir, T., 2004. “Partial Purification and Characterization of Polyphenol Oxidase from Artichoke (cynara scolymus L.) Heads,” Food Chemistry, 87, 59–67,
  • Chi, M., Bhagwat, B., Lane, W. D., Tang, G., Su, Y., Sun, R., Oomah, B. D., Wiersma, P. A., Xiang, Y., 2014. Reduced Polyphenol Oxidase Gene Expression And Enzymatic Browning In Potato (Solanum tuberosum L.) with Artificial MicroRNAs. BMC Plant Biology 14, 62.
  • Bower, J.P., Cutting, J.G.M. 1988. Influence Of Abscisic Acid On Polyphenol Oxidase Browning Potential in Avocado, South African Journal of Plant and Soil, 5(1), 42-43.
  • Coseteng, M.Y. Lee. C.Y., 1987. Changes in Apple Polyphenoloxidase and Polyphenol Concentrations in Relation to Degree of Browning. J. Food Sci. 52:985–989.
  • Espín, J. C., Morales, M., Varón, R., Tudela, J., Garciacanovas, F., 1995. A Continuous Spectrophotometric Method for Determining the Monophenolase and Diphenolase Activities of Apple Polyphenol Oxidase. Analytical Biochemistry, 231(1), 237-246.
  • Gören, N., Çağ, S., 2007. The effect of Indole-3-acetic acid and Benzyladenine on Sequential Leaf Senescence on Helianthus Annuus l. Seedlings, Biotechnology & Biotechnological Equipment, 21:3, 322-327.
  • Güleryüz, M., Ercişli, S., 1995. Kağızman İlçesinde Yetiştirilen Mahalli Elma Çeşitleri Üzerinde Biyolojik ve Pomolojik Araştırmalar. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 26(2), 183-193.
  • Holderbaum, D. F., Kon, T., Kudo, T., Guerra, M.P., 2010. Enzymatic Browning, Polyphenol Oxidase Activity, and Polyphenols in Four Apple Cultivars: Dynamics During Fruit Development. HortScience, 45(8), 1150-1154.
  • Kocaman, A., 2010. Farklı Su Streslerinde Yetiştirilen Buğday Bitkisinin (Triticum Aestivum L.) Soğuğa Dayanımını Artırmak Amacıyla Uygulanan Humik Asit, Salisik Asit Ve Absisik Asit Uygulamalarının Antioksidan Enzimler Ve Verim Unsurları Üzerine Etkisi, Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi, Erzurum.
  • Kolcuoğlu, Y., 2012.Purification and Comparative Characterization of Monophenolase and Diphenolase Activities from A Wild Edible Mushroom (macrolepiota gracilenta), Process Biochemistry, 47, 2449–2454,
  • Kołodziejczyk, K., Milala, J., Sójka, M., Kosmala, M., Markowski, J., 2010. Polyphenol Oxidase Activity in Selected Apple Cultivars. Journal of Fruit and Ornamental Plant Research, 18(2), 51-61.
  • Le Bourvellec C., Le Quere J.-M., Sanoner P., Drilleau J.-F., Guyot S., 2004. Inhibition of Apple Polyphenol Oxidase Activity by Procyanidins and Polyphenol Oxidation Products. J. Agrıc. Food Chem. 52: 122-130.
  • Lee, K.W., Kim, Y.J., Kim. D.O., Lee. H.J., Lee, C.Y., 2003. Major Phenolics in Apple and Their Contribution to the Total Antioxidant Capacity. Journal Agriculture Food Chemistry, 51(22), 6516-6520.
  • Lee, P.M., Lee, K., Karim, M.I.A., 1991. Biochemical Studies of Cocoa Bean Polyphenol Oxidase, J. Sci. Food Agric., 55. 251-260.
  • Mason, H.S., 1948. Chemistry of Melanin Mechanism of Oxidation of 3,4-Dihydroxyphenylalanine by Tyrosinase,” Journal of Biological Chemistry, 72, 83–99, 1948.Mayer, A.M., Harel, E., 1979. Polyphenol Oxidases in Plants. Phytochemistry 18, 193-214.
  • Murata, M., I. Noda, Homma, S., 1995. Enzymatic Browning of Apples on the Market: Relationship between Browning, Polyphenol Content, and Polyphenol Oxidase. Nipon Shokuhin Kagaku Kogaku Kaishi 42: 820–826 [in Japanese]
  • Olusola, L., Oluwatosin, A., 2016. Extraction of Polyphenol Oxidase from Green and Red Apple Fruits and the Effect of Ph Variation on the Activity of the Enzyme. Current Science, 2(2), 14-18
  • Podsędek, A., Wilska-Jeszka, J., Anders, B., Markowski, J., 2000. Compositional Characterisation of Some Apple Varieties. Eur. Food res. Technol. 210: 268-272.Prota, G., 1988.“Progress in the Chemistry of Melanins and Related Metabolites,” Medicinal Research Review, 8, 525–556.
  • Rocha, A.M.C.N., Morais, A.M.M.B., 2001. Characterization of Polyphenoloxidase (pfo) Extracted from ‘Jonagored’ Apple. Food Control 12: 85-90.
  • Vamos-Vigyazo, L., 1981. Polyphenol Oxidase and Peroxidase in Fruits and Vegetables. CRC Crit. Rev. Food Sci., 15, 49-127,
  • Weber, D.J., Stahmann, M.A., 1964. Ceratocystis Infection in Sweet Potato; Its Effect on Proteins, Isozyemes and Acquired Immunity. Science, 146, 929-931.
  • Williams, D.C., Lim, M.H., Chen, A.O., Pangborn, R.M., Whitaker, J.R., 1986. Blanching of Vegetables for Freezing-Which Indicator Enzyme to Choose, Food Technol., 6, 130-140,
  • Ziyan, E., 1998. "Polifenol Oksidaz Enziminin Ankara Armudu (Pyrus Communis)’dan İzole Edilmesi, Saflaştırılması ve Kinetik Özelliklerinin İncelenmesi" ,Yüksek Lisans Tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü Ankara,

Kimyasal ve elle meyve seyreltme uygulamalarının bazı elma çeşitlerinde polifenol oksidaz aktivitesi üzerine etkisi

Year 2019, Volume: 23 Issue: 2, 211 - 217, 18.06.2019

Abstract

Bu çalışmada, MM 106 klon anacına aşılı Golden Delicious, Granny Smith ve Red Chief elma
çeşitlerinde Naftalen Asetik Asit (NAA) (5,10 ppm) ve Benzyladenine (BA) (100,
150 ppm) ile kimyasal seyreltme ve elle seyreltme uygulamaları yapılmıştır.
Hasat döneminde toplanan elma çeşitlerinde uygulamalara göre polifenol oksidaz
aktivitesi belirlenmiştir. İstatistiksel analiz sonuçlarına göre çeşit,
uygulama ve çeşit x uygulama interaksiyonun polifenol oksidaz aktivitesi
üzerine etkisi önemli bulunmuştur (P<0.01). Elde edilen sonuçlara göre Red
Chief çeşidinin polifenol oksidaz aktivitesi diğer çeşitlere göre bütün
uygulamalarda daha yüksek kaydedilmiştir. NAA dozları arttıkça Golden Delicious
ve Red Chief çeşinde polifenol oksidaz enzim aktivitesi artmış, Granny Smith
çeşidinde azalmıştır. Buna karşın BA’nin dozundaki artışla birlikte Golden
Delicious ve Red Chief çeşidinde polifenol oksidaz enzim aktivitesi azalmış,
Granny Smith çeşidinde ise artmıştır.

References

  • Aydemir, T., 2004. “Partial Purification and Characterization of Polyphenol Oxidase from Artichoke (cynara scolymus L.) Heads,” Food Chemistry, 87, 59–67,
  • Chi, M., Bhagwat, B., Lane, W. D., Tang, G., Su, Y., Sun, R., Oomah, B. D., Wiersma, P. A., Xiang, Y., 2014. Reduced Polyphenol Oxidase Gene Expression And Enzymatic Browning In Potato (Solanum tuberosum L.) with Artificial MicroRNAs. BMC Plant Biology 14, 62.
  • Bower, J.P., Cutting, J.G.M. 1988. Influence Of Abscisic Acid On Polyphenol Oxidase Browning Potential in Avocado, South African Journal of Plant and Soil, 5(1), 42-43.
  • Coseteng, M.Y. Lee. C.Y., 1987. Changes in Apple Polyphenoloxidase and Polyphenol Concentrations in Relation to Degree of Browning. J. Food Sci. 52:985–989.
  • Espín, J. C., Morales, M., Varón, R., Tudela, J., Garciacanovas, F., 1995. A Continuous Spectrophotometric Method for Determining the Monophenolase and Diphenolase Activities of Apple Polyphenol Oxidase. Analytical Biochemistry, 231(1), 237-246.
  • Gören, N., Çağ, S., 2007. The effect of Indole-3-acetic acid and Benzyladenine on Sequential Leaf Senescence on Helianthus Annuus l. Seedlings, Biotechnology & Biotechnological Equipment, 21:3, 322-327.
  • Güleryüz, M., Ercişli, S., 1995. Kağızman İlçesinde Yetiştirilen Mahalli Elma Çeşitleri Üzerinde Biyolojik ve Pomolojik Araştırmalar. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 26(2), 183-193.
  • Holderbaum, D. F., Kon, T., Kudo, T., Guerra, M.P., 2010. Enzymatic Browning, Polyphenol Oxidase Activity, and Polyphenols in Four Apple Cultivars: Dynamics During Fruit Development. HortScience, 45(8), 1150-1154.
  • Kocaman, A., 2010. Farklı Su Streslerinde Yetiştirilen Buğday Bitkisinin (Triticum Aestivum L.) Soğuğa Dayanımını Artırmak Amacıyla Uygulanan Humik Asit, Salisik Asit Ve Absisik Asit Uygulamalarının Antioksidan Enzimler Ve Verim Unsurları Üzerine Etkisi, Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi, Erzurum.
  • Kolcuoğlu, Y., 2012.Purification and Comparative Characterization of Monophenolase and Diphenolase Activities from A Wild Edible Mushroom (macrolepiota gracilenta), Process Biochemistry, 47, 2449–2454,
  • Kołodziejczyk, K., Milala, J., Sójka, M., Kosmala, M., Markowski, J., 2010. Polyphenol Oxidase Activity in Selected Apple Cultivars. Journal of Fruit and Ornamental Plant Research, 18(2), 51-61.
  • Le Bourvellec C., Le Quere J.-M., Sanoner P., Drilleau J.-F., Guyot S., 2004. Inhibition of Apple Polyphenol Oxidase Activity by Procyanidins and Polyphenol Oxidation Products. J. Agrıc. Food Chem. 52: 122-130.
  • Lee, K.W., Kim, Y.J., Kim. D.O., Lee. H.J., Lee, C.Y., 2003. Major Phenolics in Apple and Their Contribution to the Total Antioxidant Capacity. Journal Agriculture Food Chemistry, 51(22), 6516-6520.
  • Lee, P.M., Lee, K., Karim, M.I.A., 1991. Biochemical Studies of Cocoa Bean Polyphenol Oxidase, J. Sci. Food Agric., 55. 251-260.
  • Mason, H.S., 1948. Chemistry of Melanin Mechanism of Oxidation of 3,4-Dihydroxyphenylalanine by Tyrosinase,” Journal of Biological Chemistry, 72, 83–99, 1948.Mayer, A.M., Harel, E., 1979. Polyphenol Oxidases in Plants. Phytochemistry 18, 193-214.
  • Murata, M., I. Noda, Homma, S., 1995. Enzymatic Browning of Apples on the Market: Relationship between Browning, Polyphenol Content, and Polyphenol Oxidase. Nipon Shokuhin Kagaku Kogaku Kaishi 42: 820–826 [in Japanese]
  • Olusola, L., Oluwatosin, A., 2016. Extraction of Polyphenol Oxidase from Green and Red Apple Fruits and the Effect of Ph Variation on the Activity of the Enzyme. Current Science, 2(2), 14-18
  • Podsędek, A., Wilska-Jeszka, J., Anders, B., Markowski, J., 2000. Compositional Characterisation of Some Apple Varieties. Eur. Food res. Technol. 210: 268-272.Prota, G., 1988.“Progress in the Chemistry of Melanins and Related Metabolites,” Medicinal Research Review, 8, 525–556.
  • Rocha, A.M.C.N., Morais, A.M.M.B., 2001. Characterization of Polyphenoloxidase (pfo) Extracted from ‘Jonagored’ Apple. Food Control 12: 85-90.
  • Vamos-Vigyazo, L., 1981. Polyphenol Oxidase and Peroxidase in Fruits and Vegetables. CRC Crit. Rev. Food Sci., 15, 49-127,
  • Weber, D.J., Stahmann, M.A., 1964. Ceratocystis Infection in Sweet Potato; Its Effect on Proteins, Isozyemes and Acquired Immunity. Science, 146, 929-931.
  • Williams, D.C., Lim, M.H., Chen, A.O., Pangborn, R.M., Whitaker, J.R., 1986. Blanching of Vegetables for Freezing-Which Indicator Enzyme to Choose, Food Technol., 6, 130-140,
  • Ziyan, E., 1998. "Polifenol Oksidaz Enziminin Ankara Armudu (Pyrus Communis)’dan İzole Edilmesi, Saflaştırılması ve Kinetik Özelliklerinin İncelenmesi" ,Yüksek Lisans Tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü Ankara,
There are 23 citations in total.

Details

Primary Language Turkish
Subjects Horticultural Production
Journal Section Araştırma Makaleleri
Authors

Ersin Gülsoy 0000-0002-4217-0695

Ayşe Türkhan This is me 0000-0002-2195-9435

Elif Duygu Kaya 0000-0001-9052-5924

Publication Date June 18, 2019
Submission Date October 26, 2018
Published in Issue Year 2019 Volume: 23 Issue: 2

Cite

APA Gülsoy, E., Türkhan, A., & Kaya, E. D. (2019). Kimyasal ve elle meyve seyreltme uygulamalarının bazı elma çeşitlerinde polifenol oksidaz aktivitesi üzerine etkisi. Harran Tarım Ve Gıda Bilimleri Dergisi, 23(2), 211-217.

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