Comparative evaluation of indigenous entomopathogenic fungal isolates and three commercial entomopathogenic fungal products against the rice weevil, Sitophilus oryzae L. and the confused flour beetle, Tribolium confusum du Val.

Year 2021, Volume: 25 Issue: 1, 1 - 12, 24.03.2021

Derya Baki , Hilal Tosun , Fedai Erler

https://doi.org/10.29050/harranziraat.814650

Cited By: 1

Abstract

In the present study, a total of 17 indigenous entomopathogenic fungal (EPF) isolates (Beauveria bassiana (Bals.) Vuill. – 14, Clonostachys rosea (Link) Schroers – 2, Isaria farinosa (Holmsk.) Fr. – 1) obtained from soil samples collected from Antalya province (southwestern part of Turkey) and three commercial EPF products [i.e. Priority® (Paecilomyces fumosoroseus), Nibortem® (Verticillium lecanii) and Nostalgist® (Beauveria bassiana)] were evaluated for their efficacy against the 7–10-day-old adults of Sitophilus oryzae L. (Coleoptera: Curculionidae) and Tribolium confusum du Val. (Col.: Tenebrionidae) under laboratory conditions. All the isolates and products were tested at 1 × 107 conidia/ml suspensions against the both insect species. The results from the single-dose pathogenicity assays showed that three B. bassiana isolates (BbDm-1, BbKp-1 and BbMp-1) caused mortalities 96.7%, 100% and 93.3% in S. oryzae, and 100%, 100% and 96.7% in T. confusum, respectively, 14 days after inoculation whereas all three commercial products achieved mortalities ranging from 56.7% and 63.3% in S. oryzae and from 56.7% and 66.7% in T. confurusum. In addition, the results from molecular phylogenetic analyses based on the ITS region sequence indicated that the three effective B. bassiana isolates (BbDm-1, BbKp-1 and BbMp-1) showed a high similarity (99%) with other B. bassiana isolates in GenBank. Overall results suggest that these three B. bassiana isolates have potential for management of S. oryzae and T. confusum.

Keywords

Beauveria bassiana,, Clonostachys rosea,, Isaria farinosa,, Sitophilus oryzae,, Tribolium confusum

Supporting Institution

Akdeniz Üniversitesi, TUBİTAK

Project Number

BAP FDK-2019-4859

Thanks

This study was financially supported by the Scientific Projects Coordination Unit of Akdeniz University (Antalya, Turkey) (Project no.: BAP FDK-2019-4859). Also, special thanks to TUBITAK BIDEB 2228-B Domestic Doctoral Fellowship Program.

Yerli entomopatojenik fungus izolatları ile üç ticari entomopatojenik fungus ürününün Sitophilus oryzae L. ve Tribolium confusum du Val üzerindeki etkinlikleri

Abstract

Bu çalışmada, Antalya ilinden toplanan toprak örneklerinden izole edilen toplam 17 yerli entomopatojenik fungus (EPF) izolatı (Beauveria bassiana (Bals.) Vuill. - 14, Clonostachys rosea (Link) Schroers - 2, Isaria farinosa (Holmsk.) Fr. - 1 ve üç ticari EPF ürünü [ör. Priority® (Paecilomyces fumosoroseus), Nibortem® (Verticillium lecanii) ve Nostalgist® (Beauveria bassiana)] laboratuvar koşulları altında Sitophilus oryzae L. (Coleoptera: Curculionidae) ve Tribolium confusum du Val. (Col .: Tenebrionidae)’un 7-10 günlük yetişkinlerine karşı etkinlikleri belirlenmiştir. Tüm EPF izolatları ve ticari ürünler, her iki böcek türüne karşı 1 × 107 conidia / ml süspansiyonlarda test edilmiştir. Tek doz patojenite testlerinin sonuçlarına göre uygulamadan 14 gün sonra sırasıyla, üç B. bassiana izolatı (BbDm-1, BbKp-1, BbMp-1) S. oryzae’de % 96,7, % 100, % 93,3 ve T. confusum’da % 100, % 100, % 96.7 ölüme neden olurken, ticari preparatlar ise S. oryzae’de % 56.7 ile % 63.3 arasında ve T. confurusum’da % 56.7 ile % 66.7 arasında değişen ölüm oranlarına neden olmuştur. Virulensliği yüksek olan üç B. bassiana izolatının (BbDm-1, BbKp-1 ve BbMp-1) ITS bölgesinin sekans dizilimleri belirlenmiş ve GenBank Blast programı kullanılarak yapılan filogenetik analizlerden elde edilen sonuçlar GenBank’taki diğer B. bassiana izolatları ile yüksek benzerlik (% 99) göstermiştir. Etki oranları yüksek olan entomopatojen fungus izolatlarının S. oryzae ve T. confusum ile mücadele de kullanılma potansiyellerinin olduğu düşünülmektedir.

Keywords

Beauveria bassiana,, Clonostachys rosea,, Isaria farinosa,, Sitophilus oryzae,, Tribolium confusum

Project Number

BAP FDK-2019-4859

References

• Adane, K., & Moore, D. S. A. (1996). Archer preliminary studies on the use of Beauveria bassiana to control Sitophilus zeamais (Coleoptera: Curculionidae) in the laboratory. Journal of Stored Products Research, 32, 105–113.
• Alramadan, Y., & Mamay, M. (2019a). The importance of entomopathogenic fungi in the control of agricultural pests and promising fungal entomopathogens in the field Application. IGAC-2019. 1st International Gobeklitepe Agriculture Congress, (pp. 266-274), 25-27 November, Şanlıurfa, TURKEY.
• Alramadan, Y., & Mamay, M. (2019b). The importance of entomopathogenic bacteria in the control of agricultural pests and promising these entomopathogens in the field. IGAC-2019. 1st International Gobeklitepe Agriculture Congress, (pp.258-265), 25-27 November, Şanlıurfa, TURKEY.
• Alramadan, Y., & Mamay, M. (2019c). What is the role of entomopathogenic viruses in the control of agricultural pests and their future in the field application? IGAC-2019. 1st International Gobeklitepe Agriculture Congress, (pp. 301-309), 25-27 November, Şanlıurfa, TURKEY.
• Alramadan, Y., & Mamay, M. (2019d). The importance of entomopathogenic nematode and their role in the control of agricultural pests. IGAC-2019. 1st International Gobeklitepe Agriculture Congress, (pp.301-309), 25-27 November, Şanlıurfa, TURKEY.
• Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Research, 25, 3389–3402.
• Barra, P., Rosso, L., Nesci, A., & Etcheverry, M. (2013). Isolation and identification of entomopathogenic fungi and their evaluation against Tribolium confusum, Sitophilus zeamais, and Rhyzopertha dominica in stored maize. Journal of Pest Science, 86, 217–226.
• Batta, Y. A., & Abu-Safieh, D. I. (2005). A study of treatment effect with Metarhizium anisopliae and four types of dusts on wheat grain infestation with red flour beetles (Tribolium castaneum Herbst, Coleoptera: Tenebrionidae). Journal of the Islamic University of Gaza, 13, 11–22.
• Batta, Y. A. (2004). Control of rice weevil (Sitophilus oryzae L., Coleoptera: Curculionidae) with various formulations of Metarhizium anisoplie. Crop Protection, 23, 103–108.
• Batta, Y. A. (2008). Control of main stored-grain insects with new formulations of entomopathogenic fungi in diatomaceous earth dusts. International Journal of Food Engineering, 4, 1556–3758.
• Batta, Y. A., & Kavallieratos, N. G. (2018). The use of entomopathogenic fungi for the control of stored-grain insects. International Journal of Pest Management, 64(1), 77–87.
• Bidochka, M. J., Kamp, A. M., & De Croos, J. N. A. (2000). Insect Pathogenic Fungi: From Genes to Populations. In Fungal Pathology; Springer: Heidelberg, The Netherlands.
• Boyer, S., Zhang, H., & Lempérière, G. (2012). A review of control methods and resistance mechanisms in stored-product insects. Bulletin of Entomological Research, 102(2), 213–229.
• Campolo, O., Giunti, G., Russo, A., & Palmeri, V. (2018). Essential oils in stored product insect pest control. Journal of Food Quality, Article ID 6906105 (18pp.).
• Cherry, A. J., Abalo, P., Hell, K., & Korie, S. (2007). Farm-scale trials to compare the entomopathogenic fungus Beauveria bassiana with pirimiphos methyl + deltamethrin and essential oil of lemon grass for protection of stored cowpea against Callosobruchus maculatus (Coleoptera: Bruchidae). Annals of Applied Biology, 151, 1–10.
• Dal Bello, G., Padin, S., López, L. C., & Fabrizio, M. (2000). Laboratory evaluation of chemical-biological control of the rice weevil (Sitophilus oryzae L.) in stored grains. Journal of Stored Products Research, 37, 77–84.
• Doyle, J. J., & Doyle, J. L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12, 13–15.
• Er, M. K., Tunazı, H., Ucuk, C., Barıs, C., & Isıkber, A. A. (2016). Occurrence of entomopathogenic fungi on insect pests of stored wheat and maize in Central and South Anatolia in Turkey. Türkiye Entomoloji Dergisi, 40(3), 249–263.
• Erler, F. (2005). Fumigant activity of six monoterpenoids from aromatic plants in Turkey against the two stored-product insects confused flour beetle, Tribolium confusum, and Mediterranean flour moth, Ephestia kuehniella. Journal of Plant Diseases and Protection, 112(6), 602–611.
• FAO. (Food and Agriculture Organization of the United Nations) (2012). Second Global Conference on Research for Agricultural Development – Breakout Section: National Food Security – The Wheat Initiative. Available online at: http://www.fao.org/docs/eims/upload//306175/Briefing%20Paper%20(3)-Wheat%20Initative%20-%20H%C3%A9l%C3%A8ne%20Lucas.pdf.
• Gaesser, G. (2014). The Value of Grains in a Healthful Diet. Available online at: http://gowiththegrain.org/pdf/TheValueofGrains.pdf.
• Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98NT. Nucleic Acids Symposium Series, 41, 95–98.
• Howe, R. W. (2008). The effects of temperature and humidity on the rate of development and the mortality of Tribolium confusum Duval (Coleoptera, Tenebrionidae). Annals of Applied Biology, 48(2), 363–376.
• Kassaye, A. (2010). Susceptibility of the rice weevil, Sitophilus oryzae (Coleoptera: Curculionidae) to native entomopathogenic fungal isolates. Addis Ababa University, Department of Biology, M.Sc. thesis, 51p, Ethiopia.
• Kavallieratos, N. G., Athanassiou, C. G., Aountala, M. M., & Kontodimas, D. C. (2014). Evaluation of the entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea for control of Sitophilus oryzae. Journal of Food Protection, 1, 4–17.
• Khashaveh, A., Ghosta, Y., Safaraziladeh, M. H., & Ziaee, M. (2011). The use of enthomopathogenic fungus, Beauveria bassiana (BALS.) VUILL. In assays with storage grain beetles. Journal of Agricultural Science and Technology, 13, 35–42.
• Kimura, M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
• Komaki, A., Kordali, S., Usanmaz, B. A., Altınok, H., Kesdek, M., Simsek, D., & Altınok, M. (2017). Laboratory assessment for biological control of Tribolium confusum du Val., 1863 (Coleoptera: Tenebrionidae) by entomopathogenic fungi. Türkiye Entomoloji Dergisi, 41(1), 95–103.
• Lacey, L. A. (2017). Microbial Control of Insect and Mite Pests: From Theory to Practice. Lawrence A. L. (edt.), 1st edit. (p. 461). Academic Press, Amsterdam, The Netherlands.
• Lord, J. C. (2009). Efficacy of Beauveria bassiana for control of Tribolium castaneum with reduced oxygen and increased carbon dioxide. Journal of Applied Entomology, 133, 101–107.
• Maina, U. M., Galadima, I. B., Gambo, F. M., & Zakaria, D. (2018). A review on the use of entomopathogenic fungi in the management of insect pests of field crops. Journal of Entomology and Zoology Studies, 6, 27–32.
• Mamay, M., Ünlü, L., Yanık, E., Doğramacı, M., & İkinci, A. (2016). Efficacy of mating disruption technique against carob moth, Apomyelois ceratoniae Zeller (Lepidoptera: Pyralidae) in pomegranate orchards in Southeast Turkey (Şanlıurfa). International Journal of Pest Management, 62(4), 295-299.
• Mamay, M., & Dağ, E. (2016). Efficacy of Mass Trapping Technique against Carob Moth [Apomyelois (= Ectomyelois) ceratoniae Zell. (Lepidoptera: Pyralidae)] in Pomegranate Orchards. II. International Multidisciplinary Congress of Eurasia, (pp. 36-41), 11-13 July, Odessa, Ukraine.
• Mamay, M., & Mutlu, Ç. (2019a). Trend biotechnological management methods against agricultural pests: mating disruption, mass trapping and attract & kill. IGAC-2019. 1st International Gobeklitepe Agriculture Congress, (pp. 511-517), 25-27 November, Şanlıurfa, TURKEY,
• Mamay, M., & Mutlu, C. (2019b). Optimizing container size and rearing density for rapid and economic mass rearing of Oenopia conglobata (Linnaeus, 1758)(Coleoptera: Coccinellidae). Türkiye Entomoloji Dergisi, 43(4), 395-408.
• Mar, T. T., Suwannarach, N., & Lumyong, S. (2012). Isolation of fungi from Northern Thailand and their production in cereal grains. World Journal of Microbiology and Biotechnology, 28, 3281–3291.
• Miller, D. W. (1995). Commercial Development of Entomopathogenic Fungi: Formulation and Delivery. (pp. 213–220). In Abstracts of Papers of the American Chemical Society; American Chemical Society: Washington, DC, USA.
• Moino, J. A., Alves, S. B., & Pereira, R. M. (1998). Efficacy of Beauveria bassiana (Bal.) Vuilemin isolates for control of stored grain pests. Journal of Applied Entomology, 122, 301–305.
• Odour, G. I., Smith, S. M., Chandi, E. A., Karanja, L. W., Agano, J. O., & Moore, D. (2000). Occurrence of Beauveria bassiana on insect pests of stored maize in Kenya. Journal of Stored Products Research, 36, 177–185.
• Padı́n, S., Dal Bello, G., & Fabrizio, M. (2002). Grain loss caused by Tribolium castaneum, Sitophilus oryzae and Acanthoscelides obtectus in stored durum wheat and beans treated with Beauveria bassiana. Journal of Stored Products Research, 38, 69–74.
• Pimentel, M., Faroni, L., Batista, M., & Silva, F. (2008). Resistance of stored-product insects to phosphine. Pesquisa Agropecuária Brasileira, 43(12), 1671–1676.
• Rajendran, S. (2002). Postharvest Pest Losses. (pp. 654–656). Encyclopedia of Pest Management, Pimentel, D. (Ed.). Marcel Dekker, Inc., New York.
• Sheeba, G., Seshadri, S., Raja, N., Janarthanan, S., & Ignacimuthu, S. (2001). Efficacy of Beauveria bassiana for control of the rice weevil Sitophilus oryzae (L.) (Coleoptera: Curculionidae). Applied Entomology and Zoology, 36, 117–120.
• Stephou, V. K., Tjamos, S. E., & Paplomatas, E. J. (2012). Transformation and attachment of Beauveria bassiana conidia on the cuticle of Tribolium confusum and Sitophilus oryzae in conjunction with diatomaceous earth. Journal of Pest Science, 85, 387–394.
• Talukder, F. (2009). Pesticide resistance in stored-product insects and alternative biorational management: a brief review. Journal of Marine Science and Technology, 14, 9–15.
• Tamura, K. (1992). Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution, 9, 678–687.
• Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.
• Tunc, I., Berger, B. M., Erler, F., & Dagli, F. (2000). Ovicidal activity of essential oils from five plants against two stored-product insects. Journal of Stored Products Research, 36(2), 161–168.
• Veraverbeke, W. S., Delcour, J. A. (2002). Wheat protein composition and properties of wheat glutenin in relation to breadmaking functionality. Critical Reviews in Food Science and Nutrition, 42, 179–208.
• White, T. J., Bruns, T., Lee, S., & Taylor, J. W. (1990). Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes For Phylogenetics. (pp. 315–322). PCR Protocols: a Guide to Methods and Applications, Academic Press, New York.
• Yıldırım, E., Özbek, H., Aslan, İ. (2001). Stored products pests. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 191.