Comparative evaluation of indigenous entomopathogenic fungal isolates and three commercial entomopathogenic fungal products against the rice weevil, Sitophilus oryzae L. and the confused flour beetle, Tribolium confusum du Val.

Year 2021, Volume: 25 Issue: 1, 1 - 12, 24.03.2021

Derya Baki Hilal Tosun Fedai Erler

https://doi.org/10.29050/harranziraat.814650

Cited By: 1

Abstract

In the present study, a total of 17 indigenous entomopathogenic fungal (EPF) isolates (Beauveria bassiana (Bals.) Vuill. – 14, Clonostachys rosea (Link) Schroers – 2, Isaria farinosa (Holmsk.) Fr. – 1) obtained from soil samples collected from Antalya province (southwestern part of Turkey) and three commercial EPF products [i.e. Priority® (Paecilomyces fumosoroseus), Nibortem® (Verticillium lecanii) and Nostalgist® (Beauveria bassiana)] were evaluated for their efficacy against the 7–10-day-old adults of Sitophilus oryzae L. (Coleoptera: Curculionidae) and Tribolium confusum du Val. (Col.: Tenebrionidae) under laboratory conditions. All the isolates and products were tested at 1 × 107 conidia/ml suspensions against the both insect species. The results from the single-dose pathogenicity assays showed that three B. bassiana isolates (BbDm-1, BbKp-1 and BbMp-1) caused mortalities 96.7%, 100% and 93.3% in S. oryzae, and 100%, 100% and 96.7% in T. confusum, respectively, 14 days after inoculation whereas all three commercial products achieved mortalities ranging from 56.7% and 63.3% in S. oryzae and from 56.7% and 66.7% in T. confurusum. In addition, the results from molecular phylogenetic analyses based on the ITS region sequence indicated that the three effective B. bassiana isolates (BbDm-1, BbKp-1 and BbMp-1) showed a high similarity (99%) with other B. bassiana isolates in GenBank. Overall results suggest that these three B. bassiana isolates have potential for management of S. oryzae and T. confusum.

Keywords

Beauveria bassiana,, Clonostachys rosea,, Isaria farinosa,, Sitophilus oryzae,, Tribolium confusum

Supporting Institution

Akdeniz Üniversitesi, TUBİTAK

Project Number

BAP FDK-2019-4859

Thanks

This study was financially supported by the Scientific Projects Coordination Unit of Akdeniz University (Antalya, Turkey) (Project no.: BAP FDK-2019-4859). Also, special thanks to TUBITAK BIDEB 2228-B Domestic Doctoral Fellowship Program.

Yerli entomopatojenik fungus izolatları ile üç ticari entomopatojenik fungus ürününün Sitophilus oryzae L. ve Tribolium confusum du Val üzerindeki etkinlikleri

Abstract

Bu çalışmada, Antalya ilinden toplanan toprak örneklerinden izole edilen toplam 17 yerli entomopatojenik fungus (EPF) izolatı (Beauveria bassiana (Bals.) Vuill. - 14, Clonostachys rosea (Link) Schroers - 2, Isaria farinosa (Holmsk.) Fr. - 1 ve üç ticari EPF ürünü [ör. Priority® (Paecilomyces fumosoroseus), Nibortem® (Verticillium lecanii) ve Nostalgist® (Beauveria bassiana)] laboratuvar koşulları altında Sitophilus oryzae L. (Coleoptera: Curculionidae) ve Tribolium confusum du Val. (Col .: Tenebrionidae)’un 7-10 günlük yetişkinlerine karşı etkinlikleri belirlenmiştir. Tüm EPF izolatları ve ticari ürünler, her iki böcek türüne karşı 1 × 107 conidia / ml süspansiyonlarda test edilmiştir. Tek doz patojenite testlerinin sonuçlarına göre uygulamadan 14 gün sonra sırasıyla, üç B. bassiana izolatı (BbDm-1, BbKp-1, BbMp-1) S. oryzae’de % 96,7, % 100, % 93,3 ve T. confusum’da % 100, % 100, % 96.7 ölüme neden olurken, ticari preparatlar ise S. oryzae’de % 56.7 ile % 63.3 arasında ve T. confurusum’da % 56.7 ile % 66.7 arasında değişen ölüm oranlarına neden olmuştur. Virulensliği yüksek olan üç B. bassiana izolatının (BbDm-1, BbKp-1 ve BbMp-1) ITS bölgesinin sekans dizilimleri belirlenmiş ve GenBank Blast programı kullanılarak yapılan filogenetik analizlerden elde edilen sonuçlar GenBank’taki diğer B. bassiana izolatları ile yüksek benzerlik (% 99) göstermiştir. Etki oranları yüksek olan entomopatojen fungus izolatlarının S. oryzae ve T. confusum ile mücadele de kullanılma potansiyellerinin olduğu düşünülmektedir.

Keywords

Beauveria bassiana,, Clonostachys rosea,, Isaria farinosa,, Sitophilus oryzae,, Tribolium confusum

Project Number

BAP FDK-2019-4859

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