Bazı endofitik ve rizosferik bakterilerin fasulyede Macrophomina phaseolina’ya karşı etkinliklerinin in vitro koşullarda belirlenmesi

Year 2023, Volume: 27 Issue: 1, 42 - 51, 24.03.2023

Raziye Koçak , Özden Salman

https://doi.org/10.29050/harranziraat.1195672

Abstract

Macrophomina phaseolina (Tassi) Goid. geniş konukçu aralığına sahip toprak kökenli bir hastalık olup mücadelesi oldukça zordur. Bundan dolayı, çalışmamızda 2021 yılında Konya’nın Çumra ilçesinden alınan fasulye bitkilerinin farklı aksamlarından (çiçek, gövde, kök ve yaprak) ve topraktan elde edilen toplam 71 bakterinin hastalığa karşı in vitro etkinliklerinin belirlenmesi amaçlanmıştır. İkili kültür testlerinde %60 ve üzerinde etki gösteren bakteriler için bazı biyokimyasal testler (Hidrojen siyanid aktivitesi, fosforu çözme yeteneği, siderofor sentez kabiliyeti, indol asetik asit üretebilme yetenekleri ve ACC deaminaz aktivitelerinin belirlenmesi) uygulanmıştır. Deneme sonucunda değerlendirmeler yapılmış ve bu testler puanlandırılarak en yüksek etkiyi gösteren bakteriler daha sonraki çalışmalarda kullanılmak üzere seçilmiştir. Macrophomina phaseolina’ya karşı ikili kültür denemelerinde başarılı olan izolatlar; yapraktan izole edilen Bacillus pumilus DP 25 (%91), rizosferik Bacillus subtilis DP 143.6 (%86) ve rizosferik Bacillus cereus DP 145.1 (%100) olmuştur. Yüksek düzeyde etkili olan bu izolatların fosforu çözme kabiliyetleri, siderofor sentez yetenekleri ve indol asetik asit kabiliyetleri pozitif olarak belirlenmiştir.

Keywords

Bacillus, Macrophomina, Siderofor, İn vitro, Fasulye

Supporting Institution

Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

21401059 nolu proje

Thanks

Bu çalışma, Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından desteklenmiş olan 21401059 nolu proje kapsamında yapılmıştır.

In vitro determination of efficacies of some endophytic and rhizospheric bacteria against Macrophomina phaseolina in beans

Abstract

Macrophomina phaseolina (Tassi) Goid is a soil-borne disease with a wide host range and is very difficult to control. In our study, a total of 71 bacteria were obtained from different parts (flower, stem, root and leaf) of bean plants and soil taken from Çumra district of Konya in 2021 and in vitro efficacies of bacteria against disease were determined. Some biochemical tests (hydrogen cyanide activity, phosphorus solubilization ability, siderophore synthesis ability, indole acetic acid production ability and determination of ACC deaminase activities) were applied for bacteria that had an effect of 60% or more in double culture tests. At the end of the experiment, we were evaluated our data and these tests were scored and the bacteria showing the highest effect were selected for use in further studies. Isolates that were successful in dual culture tests against Macrophomina phaseolina were Bacillus pumilus DP 25 (91%), rhizospheric Bacillus subtilis DP 143.6 (86%), and rhizospheric Bacillus cereus DP 145.1 (%100) isolated from leaves. Phosphorus solubility, siderophore synthesis abilities and indole acetic acid abilities of these highly effective isolates were determined as positive.

Keywords

Bacillus, Macrophomina, Siderophore, in vitro, Bean

Project Number

21401059 nolu proje

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