Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris

Year 2023, Volume: 27 Issue: 1, 64 - 72, 24.03.2023

Murat Ozturk , Ali Endes

https://doi.org/10.29050/harranziraat.1216304

Cited By: 1

Abstract

In 2021, post-harvest soil samples were taken from fields where chickpea and wheat were grown in Yozgat province to isolate bacteria. Of 74 isolates obtained from soil samples, 14 nonpathogenic isolates were evaluated for their inhibitory potential against two pathogenic isolates (YBUFoc9 and YBUFoc2) of Fusarium wilt disease agent Fusarium oxysporum f. sp. ciceris. Antagonistic bacterial isolates were identified as Bacillus spp. by Blastn analysis based on their 16S rDNA nucleotide sequences. Among them, 2 isolates were identified as B. amyloliquefaciens (BM23 and BM40), 3 isolates as B. subtilis (BM8, BM32, and BM105), 6 isolates as B. cereus (BM10, BM69, BM70, BM104, BM111 and BM215), 2 isolates as B. megaterium (BM44 and BM135) and 1 isolate as B. pumilus (BM28). No effective isolate of any genus other than Bacillus spp. was found. They inhibited mycelial growth of isolates YBUFoc9 and YBUFoc2 from 0 to 78.50% and from 0 to 58.33%, respectively. Among the 5 different species, B. amyloliquefaciens (BM23 and BM40) and B. subtilis (BM8, BM32, and BM105) showed the strongest inhibitory activity against the two fungal isolates under in vitro conditions. In further studies, the biocontrol properties of the most effective isolates (BM23, BM40, BM8, BM32 and BM105) will be investigated and compared with approved fungicides for their fungicidal activity.

Keywords

Fusarium wilt, Chickpea, Bacillus spp., Biological control

Thanks

We would like to thank Prof. Dr. Soner Soylu for valuable advice on experimental design and important criticisms of the manuscript and Dr. Ali Kayahan for statistical analysis.

Toprak örneklerinden bakteri izolatlarının izolasyonu ve nohut solgunluğu hastalığına neden olan Fusarium oxysporum f. sp. ciceris’e karşı in vitro antagonistik potansiyellerinin belirlenmesi

Abstract

2021 yılında Yozgat ilinde nohut ve buğday yetiştirilen tarlalardan bakteri izolasyonu için hasat sonrası toprak örnekleri toplanmıştır. Toprak örneklerinden izole edilen 74 izolat arasından patojenik olmayan 14 aday bakteri izolatın Fusarium solgunluğu etmeni Fusarium oxysporum f. sp. ciceris’in iki izolatına (YBUFoc9 and YBUFoc2) karşı engelleme potansiyelleri in vitro koşullarda değerlendirilmiştir. Antagonist bakteri izolatların tanısı 16S rDNA nükleotid dizilerine dayanan Blastn analizi ile Bacillus spp. olarak belirlenmiştir. Elde edilen izolatların 2'si B. amyloliquefaciens (BM23 ve BM40), 3'ü B. subtilis (BM8, BM32 ve BM105), 6'sı B. cereus (BM10, BM69, BM70, BM104, BM111 ve BM215), 2’si B. megaterium olarak (BM44 ve BM135) ve 1’i B. pumilus (BM28) olarak tanılanmıştır. Antagonistik etkinlik bakımından Bacillus spp. dışında etkili bir cinse rastlanılmamıştır. Bakteriyel izolatlar, YBUFoc9 ve YBUFoc2 izolatlarının misel büyümesine sırasıyla %0-%78.50 ve %0-%58.33 oranında inhibisyon sağlamışlardır. Beş farklı tür arasından B. amyloliquefaciens (BM23 ve BM40) ve B. subtilis’in (BM8, BM32 ve BM105) in vitro koşullar altında patojenin iki izolatına karşı en güçlü inhibisyon aktivitesi gösterdiği belirlenmiştir. Gelecekte yapılması planlanan çalışmalarda, en etkili izolatların (BM23, BM40, BM8, BM32 ve BM105) biyokontrol özellikleri araştırılacak ve fungisidal aktiviteleri için onaylanmış fungisitlerle karşılaştırılacaktır.

Keywords

Fusarium solgunluğu, Nohut, Bacillus spp., Biyolojik mücadele

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