Aspir çeşit ve ıslah hatlarında AFLP belirteçleri ile genetik çeşitliliğin belirlenmesi

Year 2023, Volume: 27 Issue: 2, 145 - 152, 23.06.2023

Muhammet Tonguç Sabri Erbaş

https://doi.org/10.29050/harranziraat.1248981

Abstract

Aspir, kendine döllenen tek yıllık bir yağ bitkisidir ve antik çağlardan beri Orta Doğu'da yetiştirilmektedir. Aspir önemini yitiren bir kültür bitkisi haline gelmiştir ve aspir tarımını artırmak için yeni çeşitlerin temini ve karakterizasyonu gereklidir. Farklı coğrafi kökene sahip 38 aspir çeşidi ve ıslah hattı arasındaki genetik çeşitliliği ve ilişkilerini belirlemek için 8 AFLP primer kombinasyonu kullanılarak genotiperler taranmıştır. AFLP primerleri, reaksiyon başına ortalama 51.2 bant ve toplamda 410 bant üretmiştir. Üretilen bantların 113 tanesinin genotipler arasında polimorfik olduğu gözlenmiş ve polimorfizm oranı %14.8-%41.4 arasında değişmiştir. Primer kombinasyonlarının polimorfizm bilgi içeriği (PIC) 0.21-0.38 ve çözümleme gücü (Rp) 5.11-27.68 arasında değişmiştir. Aspir çeşit ve ıslah hatları arasındaki genetik benzerlik katsayıları 0.245-0.850 arasında değişkenlik gösterirken; ortalama genetik benzerlik 0.573 olarak bululnumuştur. Benzerlik matrisine dayalı dendogram, genotipleri üç küme içine yerleştirmiş fakat üç kültivar gruplandrılamamıştır. Mevcut çalışma, genotipler içerisindeki genetik çeşitliliğin boyutunu göstermekte ve gelecekte yapılacak ıslah ve genetik analizler için faydalı olacaktır.

Keywords

Carthamus tinctorius, Aspir, Genetik çeşitlilik, Moleküler Belirteç

Supporting Institution

Türkiye Bilimsel ve Teknik Araştırma Kurumu (TÜBİTAK)

Project Number

107O504

Assessment of genetic diversity in safflower cultivars and breeding lines with AFLP markers

Abstract

Safflower is a self-pollinating oilseed crop and has been cultivated since antiquity in the Middle East. Safflower has become a minor oilseed crop, and to increase its cultivation it is necessary to characterize and introduce new germplasm. Thirty-eight safflower cultivars and breeding lines from different countries have been screened with 8 AFLP primer combinations to assess genetic diversity and relationships among these genotypes. AFLP primers produced total of 410 bands, 113 of which was polymorphic, with an average of 51.2 bands per assay. Polymorphism ratio changed between 14.8 to 41.4% among the genotypes. Polymorphism information content (PIC) and resolving power (Rp) of the primer combinations were between 0.21-0.38 and 5.11-27.68, respectively. Genetic similarity coefficients were between 0.245-0.850 with an average of 0.573. Dendrogram based on similarity matrix produced three clusters and three cultivars clustered separately from the rest of the genotypes. The study shows genetic variation within germplasm and could be useful for breeding and genetic diversity studies in the future.

Keywords

Carthamus tinctorius, Safflower, Molecular markers, Genetic diversity

Project Number

107O504

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