Rhizoctonia solani'nin mücadelesinde At Kestanesi ağacından izole edilen Fusarium oxysporum'un endofit olarak antagonistik potansiyelinin in-vitro koşullarda belirlenmesi

Year 2024, Volume: 28 Issue: 4, 550 - 563, 15.12.2024

Muhammad Arif

https://doi.org/10.29050/harranziraat.1524993

Abstract

Endofitik mikroorganizmalar, konakçılarıyla simbiyotik ilişkiler kurarak bitki patojenlerine karşı koruma gibi çeşitli avantajlar sağlamaktadırlar. Toprak kaynaklı patojenler, birçok bitki türünde yıkıcı bir enfeksiyon kaynağı haline gelmiştir. Ancak, bu toprak kaynaklı patojenlerin mücadelesinde kullanılan çevre dostu yaklaşımlar sınırlıdır. Bu çalışma, fungal endofitik mikroorganizma (Fusarium oxysporum) kullanarak toprak kaynaklı patojeni olanı Rhizoctonia solani’nın mücadelesi amaçlamıştır. Endofitik mikroorganizma, at kestanesi ağacının yapraklarından izole edilmiştir. R. solani, kök çürüklüğü ve fide devrilmesi belirtileri gösteren sebze bitkilerinden elde edilmiştir. Hem endofitik mikroorganizma hem de patojen uygun kültür ortamlarında yetiştirilmiştir. Toplanan endofitik mikroorganizmanın toprak kaynaklı patojen üzerindeki antagonistik aktivitesi, in-vitro koşulları altında ortalama inhibisyon bölgesi tekniği ile kontrol edilmiştir. Çeşitlilik indeksleri ve izolasyon sıklığı analizi, bu ağaç türünün çeşitli bir endofitik yelpazeye sahip olduğunu ortaya koymuştur. Endofitik mikroorganizmaların toprak kaynaklı patojene (R. solani) karşı antagonistik aktivitesini değerlendiren çift kültür deneyi, endofitik mikroorganizmanın, değerlendirme zamanlarının ve endofitik mikroorganizma ile değerlendirme zamanları arasındaki etkileşimin patojen koloni boyutu üzerinde önemli (P <0.001) bir etkisi olduğunu ortaya koymuştur. Endofitik mikroorganizma, inokülasyondan sonraki 11 ve 15 günler dışında, kontrol grubuna kıyasla patojen gelişiminde önemli bir azalma göstermiştir. Veriler, F. oxysporum'un endofitik potansiyele sahip olduğunu ve diğer toprak kaynaklı hastalıklara karşı potansiyel biyolojik mücadele ajanları olarak araştırılabileceğini göstermektedir.

Keywords

Kestane ağacı, Endofit, Fusarium oxysporum, Çeşitlilik indeksleri, Rhizoctonia solani, antagonistik potansiyel

Supporting Institution

Sakarya University of Applied Sciences

Project Number

BAP-179-2023, Sakarya University of Applied Sciences, Sakarya, Türkiye.

Antagonistic potential of Fusarium oxysporum as an endophyte isolated from Horse-chestnut tree in the management of Rhizoctonia solani under in-vitro conditions

Abstract

Symbiotic relationships are established by endophytic microorganisms with their host, resulting in the provision of diverse advantages, such as protection against plant pathogens. Soil-borne pathogens have become a devastating source of infection in many plant species. The environmentally friendly approaches are scare in managing these soil-borne pathogens. This study was aimed to manage one soil-borne pathogen (Rhizoctonia solani) by employing another fungal endophyte (Fusarium oxysporum) via mean inhibition zone technique. The fungal endophyte was isolated from horse-chestnut tree leaves. The R. solani was extracted from the vegetable’s plants showing typical symptoms of root rot and damping off. Both endophyte and pathogen were grown on suitable culture media. The antagonistic activity of collected endophyte for soil-borne pathogen was checked via mean inhibition zone technique under in-vitro condition. The diversity indices and isolation frequency analysis revealed that this tree specie has versatile endophytic range. The results from the dual culture experiment assessing the antagonistic activity of endophyte against the soil-borne pathogen (R. solani) revealed a significant (P <0.001) impact of the endophyte, evaluation times, and the interaction between endophyte and evaluation times on the size of the pathogen colony. The endophyte exhibited a substantial decrease in pathogen development compared to the control, except between days 11 and 15 after inoculation. The data indicate that F. oxysporum contains endophytic potential, which might be investigated for potential biocontrol agents against other soil-borne diseases.

Keywords

Chestnut tree, Endophyte, Fusarium oxysporum, Diversity indices, Rhizoctonia solani, antagonistic potential

Project Number

BAP-179-2023, Sakarya University of Applied Sciences, Sakarya, Türkiye.

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