Development of Meloidogyne chitwoodi resistant potato lines

Year 2025, Volume: 29 Issue: 2, 228 - 236, 16.06.2025

Ercan Özkaynak

https://doi.org/10.29050/harranziraat.1638568

Abstract

Meloidogyne chitwoodi is an important root-knot nematode in some potato production regions of Türkiye. Crop rotation and some chemical and cultural control methods remain ineffective in controlling this nematode. Developing resistant varieties is an environmentally safe and most effective solution for M. chitwoodi. The current study was aimed at the development of M. chitwoodi-resistant potato lines and varieties adapted to different regions. Two breeding populations (‘22-99: PP-89 X Maradonna’, ‘22-108: PP-85 X Banba’) and two offspring populations (‘32-12: 22-99-33 X Provento’; ‘32-10: 22-108-24 X Saturna’) were used as genetic material. Meloidogyne chitwoodi molecular marker and field pathogenicity tests were used for investigation of M. chitwoodi-resistant lines and varieties. The results of the molecular marker and field tests were compatible. A total of 112 potato breeding lines were evaluated In two populations and 81 were resistant to M. chitwoodi. Consequently, potato line of number 33 from the 22-99 population and potato line of number 24 from the 108-24 population, along with their superior agronomic and yield characteristics, were chosen as M. chitwoodi-resistant lines according to the marker and field test studies. Overall the results suggest that resistant varieties can be improved by large-scale molecular markers and field tests.

Keywords

Molecular marker , Root-knot nematode , Solanum tuberosum L. , Potato lines , Nematode resistance

Meloidogyne chitwoodi'ye dayanıklı patates hatlarının geliştirilmesi

Abstract

Meloidogyne chitwoodi Türkiye'nin bazı patates üretim bölgelerinde önemli bir kök ur nematodudur. Ekim nöbeti, bazı kimyasal ve kültürel uygulamalar bu nematodla mücadelede etkili olmamıştır. Meloidogyne chitwoodi'ye karşı çevre açısından güvenli ve etkili en iyi yöntem dayanıklı çeşitlerin geliştirilmesidir. Bu araştırmanın amacı, M. chitwoodi'ye karşı farklı bölgelere adapte olmuş dayanıklı patates hatları ve çeşitleri geliştirmek olmuştur. Denemede genetik materyal olarak iki ıslah popülasyonu (22-99: PP-89 X Maradonna, 22-108: PP-85 X Banba) ve ikinci döl popülasyonu (‘32-12: 22-99-33 X Provento’; ‘32-10: 22-108-24 X Saturna’) kullanılmıştır. Nematod dayanıklı hat ve çeşitlerin geliştirilmesinde M. chitwoodi moleküler markeri ve tarla patolojik testleri kullanılmıştır. Moleküler marker testi ve tarla testlerinin sonuçları uyumlu gözükmüştür. İki popülasyonda 112 patates ıslah hattı değerlendirilmiş olup bunların 81'i M. chitwoodi nematoduna dayanıklıdır. Araştırma sonucunda 22-99 popülasyonuna ait 33 numaralı patates hattı ve 108-24 popülasyonuna ait 24 numaralı hatlar, üstün agronomik ve verim özelliklerine sahip olmaları yanında, marker ve tarla deneme çalışmalarına göre M. chitwoodi'ye dayanıklı hat olarak seçilmiştir. Bu çalışmanın devamında, geniş çaplı moleküler marker test ve tarla denemeleri sonrasında dayanıklı çeşitler geliştirilebilecektir.

Keywords

Moleküler Marker , Kök-ur Nematod , Solanum tuberosum L. , Patates hattı , Nematod dayanıklılığı

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