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Lenoks (Brassica rapa) hasılının bakteriyel inokulant+enzim karışımıyla silolanmasının silaj kalitesi, in vitro sindirilebilirlik ve enerji içeriğine etkileri

Year 2025, Volume: 29 Issue: 3, 506 - 515, 24.09.2025
https://doi.org/10.29050/harranziraat.1693247

Abstract

Bu çalışma, iki farklı vejetasyon döneminde biçilen lenoks (Brassica rapa L.) bitkisinin, bakteriyel inokulant+enzim karışımı (0, 2.5, 5, and 7.5 mg.kg-1) ile silolanmasının, silaj kalitesi, in-vitro sindirilebilirlik ve enerji içeriklerinin belirlenmesi amacıyla yürütülmüştür. Çalışmada, vejetasyon döneminin gruplar arasındaki farklılığa etkisinin nötr deterjan lif (NDF) değeri hariç, önemli olduğu (p<0.05); vejetasyon döneminin ilerlemesi ile kuru madde (KM), organik madde (OM), ham yağ (HY), NDF ve asit deterjan lif (ADF) içerikleri artarken, ham kül (HK) ve ham protein (HP) düzeylerinin azaldığı belirlenmiştir (p<0.05). Vejetasyon döneminin ilerlemesiyle, silaj LA, AA ve PA değerlerinin azaldığı; NH3-N, pH ve Fleig puanı değerlerinin ise yükseldiği belirlenmiştir (p<0.05). Silajlara katılan katkı düzeylerinin etkisi, LA, AA, pH ve Fleig puanları bakımından önemli bulunmuştur (p<0.05). Lenoks silajlarının in-vitro KM (KMS) ve OM sindirilebilirlikleri (OMS) ile enerji içerikleri açısından, vejetasyon dönemi ve katkıların etkisi önemli bulunmuştur (p<0.05). Vejetasyon döneminin ilerlemesiyle, silajların KMS ve OMS değerleri azalmıştır. Silajlarda bakteriyel inokulant+enzim katkısı, kontrol grubu ile kıyaslandığında, en yüksek KMS, metabolik enerji (ME) ve net enerji laktasyon (NEL) değeri 2.5 mg.kg-1 katkılı silaj grubunda elde edilmiştir. Çalışmada, vejetasyonun ilerlemesine paralel olarak, silajların UYA, KMS ve OMS değerleri ile SE, ME ve NEL değerlerinin azaldığı; lenoks bitkisinin çiçeklenme döneminde biçilerek 2.5 mg.kg-1 bakteriyel inokulant+enzim ile silolanması durumunda, elde edilen silajların kaliteli silaj niteliği taşıdıkları ve ruminantların beslenmesinde alternatif kaliteli bir kaba yem kaynağı olarak kullanılabileceği sonucuna varılmıştır.

References

  • ANKOM. (2022). Operator’s Manual ANKOM ll 200/220 Fiber Analyzer. ANKOM Technology Corp. Fairport, NY. https://www.ankom.com/
  • Anonymous. (2024). Kjeldahl method for determining nitrogen. Behr Labor-Technik GmbH, Spangerstraße 8, 40599 Düsseldorf/Germany. Available at: https://behr-labor.com/files/behr-labor_files/analyse-loesungen/06%20Downloads/de/ lebens-_und_futtermittel_analytik/Brochure_Kjeldahl_method.pdf (Accessed OCT 10, 2024).
  • AOAC. (2019). Official methods of analysis of AOAC International. Association of Official Analytical Chemists (AOAC), Rockville, Maryland, USA.
  • Ayan, İ., Aşcı, Ö.Ö., Başaran, U. & Mut, H. (2006). Quality characters of some turnip (Brassica rapa L.) cultivars. Ondokuz Mayıs University, Journal of Faculty of Agriculture, 21 (3): 310-313.
  • Balakhial, A., Naserian, A.A., Heravi Moussavi, A., Eftekhar Shahrodi, F. & Vali Zadeh, R. (2008). Changes in chemical composition and in vitro DM digestibility of urea and molasses treated whole crop canola silage. Journal of Animal and Veterinary Advances. 7(9): 1042-1044.
  • Başkavak, S., Özdüven, M.L., Polat, C. & Koç, F. (2008). The effects of lactic acid bacteria-enzyme mixture silage inoculant on wheat silage. Journal of Tekirdag Agricultural Faculty. 5 (3), 291-296.
  • Can, L. (2010). The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro organic matter digestibility characteristics of triticale: hungarian vetch silages. MSc. Thesis. Namık Kemal University, Graduate School of Natural and Applied Sciences, Main Science Division of Animal Science. Tekirdağ
  • Çetin, İ. (2017). Determination of some quality properties of ensiling turnip (Brassica rapa L.) with different additives. MSc. Thesis. Uşak University Institute of Science and Technology, Uşak
  • Daş, B.D. (2019). The effect of different levels of wheat straw and dried molasses sugar beet pulp on the silage quality, live weight gain in lambs and digestibility of lenox (Brassica rapa L.) silage. PhD. Thesis. Harran University, Health Sciences Institute, Animal Nutrition and Nutritional Diseases Department. Şanlıurfa
  • Doğan-Daş, B. & Denek, N. (2021). Effect of wheat straw and molasses supplementation on quality, lamb performance, and digestibility of forage turnip (Brassica rapa) silage. Iranian Journal of Applied Animal Science, 11(3), 547-555
  • Filya, İ. (2002). The effects of lactic acid bacteria and lactic acid bacteria-enzyme mixture silage inoculants on maize silage. Turkish Journal of Veterinary and Animal Sciences, 26;679-687
  • İke, F. (2019). The effects of lactic acid bacteria and enzyme additive on the fermentation with aerobic stability of common vetch, wheat, oat mixture silages. MSc. Thesis. Tekirdağ Namık Kemal University, Graduate School of Natural and Applied Science. Tekirdağ
  • Ishler, V., Heinrichs, J. & Varga, G. (2016). From feed to milk: Understanding rumen function, Penn State Uni. College of Agricultural Sci. Extension Circular. 422, USA.
  • Kılıc, U. & Erisek, A. (2019). Effects of additive use on silage quality and in vitro digestibility of some brassica silages. Journal of Scientific and Engineering Research, 6(11):163-171
  • Kılıç, Ü. (2009). Using canola forages as roughage source in ruminant nutrition. Journal of Lalahan Livestock Research Institute, 49 (2): 125-135.
  • Kılıç, A. (1986). Silo Yemi. Bilgehan Basımevi. İzmir.
  • King, G. (2022). Animal Nutrition. Animal and Poultry Science, University of Guelph. Available at: https://animalbiosciences.uoguelph.ca/~gking/Ag_2350/nutrition. htm (Accessed NOV 25, 2021).
  • Kurşun, Z. (2009). The effects of bacterial inoculants on the fermentation, aerobic stability and in vitro organic matter digestibility characteristics of triticale silages. MSc. Thesis Namık Kemal University, Graduate School of Natural and Applied Sciences, Tekirdağ
  • NRC. (2021). Nutrient requirements of dairy cattle: eighth revised edition. Washington, DC: The National Academies Press. 502 pages. Available at: https://doi.org/10.17226/25806 (Accessed SEP 05, 2021).
  • Nursoy, H. & Şahin, E. (2017). Son metodlara göre yemlerin kuru madde analizleri. Tr. Doğa ve Fen Derg. − Tr. J. Nature Sci. 2017 Vol. 6 No. 1
  • Özkan, F. (2019). Determining the quality of sugar beet pulp, lenox and ryegrass silages used in feeding dairy cattle, in comparison with corn silage, Ph.D. Thesis, Van Yüzüncü Yıl University, Van, Turkey
  • Suzuki, M. &, Lund, C.W. (1980). Improved gas-liquid chromatography for simultaneous determination of volatile fatty acids and lactic acid in silage. Journal of Agricultural and Food Chemistry, 28, 1040-1
  • Türk, M., Albayrak, S., Balabanlı, C. & Yüksel, O. (2009). Effects of fertilization on root and leaf yields and quality of forage turnip (Brassica rapa L.). Journal of Food, Agriculture and Environment, 7(3-4): 339-342.
  • Van-Soest, P.J., Robertson, J.B. & Lewis, B.A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10): 3583-3597. doi:10.3168/jds.S0022-0302(91)78551-2.
  • Yaylak, E. & Alçiçek, A. (2003). Sığır besiciliğinde ucuz bir kaba yem kaynağı: mısır silajı. Hayvansal Üretim. 44(2), 29-36.
  • Yıldız, S., Deniz, S., Özkan, F. & Kale, Ç. (2022). Forage turnip (Brassica rapa) harvested in different phases of vegetative stage and ensiled with the additives of molasses and barley and the effects of additives on silage quality, in vitro digestibility, and energy content. Turkish Journal of Veterinary & Animal Sciences 46 (3), 475-482 DOI: 10.55730/1300-0128.4196
  • Yıldız, S., Deniz, S., Özkan, F. & Kale, Ç. (2023). Effects of ensiling forage turnip (Brassica Rapa) plant harvested in different vegetation periods with triticale (X Triticosecale Wittmack) forage at different ratios on silage quality in vitro digestibility and energy content. Journal of Animal and Plant Science, 33 (4). 855-863 https://doi.org/10.36899/JAPS.2023.4.0678

Determination of the silage quality of Lenox (Brassica rapa) product harvested in different periods

Year 2025, Volume: 29 Issue: 3, 506 - 515, 24.09.2025
https://doi.org/10.29050/harranziraat.1693247

Abstract

The objective of this study was to identify the impact of making silages using Lenox (forage turnip, Brassica rapa L.) harvested in two different stages of vegetation with bacterial inoculant+enzyme mixtures (0, 2.5, 5, and 7.5 mg.kg-1) on the quality, in-vitro digestibility, and energy parameters of the silages. The vegetation period affected the examined variables significantly except for neutral detergent fiber (NDF), (p<0.005), and further into the vegetation period, dry matter (DM), organic matter (OM), ether extract (EE), NDF, acid detergent fiber (ADF), NH3-N, pH, and Fleig score increased, whereas ash, crude protein (CP), lactic acid (LA), acetic acid (AA), and propionic acid (PA) decreased (p<0.005). The effect of additive levels added to the silages was found to be significant in terms of LA, AA, pH and fleig scores (p<0.05). The effects of the vegetation period and additives were found to be significant in terms of in vitro DM (DMD) and OM digestibility (OMD) and energy contents of Lenox silages (p<0.05). Further into the vegetation period, DMD and OMD values decreased. Compared to the control group, the greatest DMD, metabolic energy (ME), and net energy for lactation (NEL) results were found in the group with 2.5 mg.kg-1 of additive. It was concluded that the VFA, DMD, OMD, SE, ME, and NEL results of the silage samples dropped as the vegetation period progressed, and when forage turnip was ensiled in its flowering period with the bacterial inoculant+enzyme mixture at a ratio of 2.5 mg.kg-1, the quality of the obtained silages was high, and these can be utilized as alternative, quality roughage sources for feeding ruminants.

References

  • ANKOM. (2022). Operator’s Manual ANKOM ll 200/220 Fiber Analyzer. ANKOM Technology Corp. Fairport, NY. https://www.ankom.com/
  • Anonymous. (2024). Kjeldahl method for determining nitrogen. Behr Labor-Technik GmbH, Spangerstraße 8, 40599 Düsseldorf/Germany. Available at: https://behr-labor.com/files/behr-labor_files/analyse-loesungen/06%20Downloads/de/ lebens-_und_futtermittel_analytik/Brochure_Kjeldahl_method.pdf (Accessed OCT 10, 2024).
  • AOAC. (2019). Official methods of analysis of AOAC International. Association of Official Analytical Chemists (AOAC), Rockville, Maryland, USA.
  • Ayan, İ., Aşcı, Ö.Ö., Başaran, U. & Mut, H. (2006). Quality characters of some turnip (Brassica rapa L.) cultivars. Ondokuz Mayıs University, Journal of Faculty of Agriculture, 21 (3): 310-313.
  • Balakhial, A., Naserian, A.A., Heravi Moussavi, A., Eftekhar Shahrodi, F. & Vali Zadeh, R. (2008). Changes in chemical composition and in vitro DM digestibility of urea and molasses treated whole crop canola silage. Journal of Animal and Veterinary Advances. 7(9): 1042-1044.
  • Başkavak, S., Özdüven, M.L., Polat, C. & Koç, F. (2008). The effects of lactic acid bacteria-enzyme mixture silage inoculant on wheat silage. Journal of Tekirdag Agricultural Faculty. 5 (3), 291-296.
  • Can, L. (2010). The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro organic matter digestibility characteristics of triticale: hungarian vetch silages. MSc. Thesis. Namık Kemal University, Graduate School of Natural and Applied Sciences, Main Science Division of Animal Science. Tekirdağ
  • Çetin, İ. (2017). Determination of some quality properties of ensiling turnip (Brassica rapa L.) with different additives. MSc. Thesis. Uşak University Institute of Science and Technology, Uşak
  • Daş, B.D. (2019). The effect of different levels of wheat straw and dried molasses sugar beet pulp on the silage quality, live weight gain in lambs and digestibility of lenox (Brassica rapa L.) silage. PhD. Thesis. Harran University, Health Sciences Institute, Animal Nutrition and Nutritional Diseases Department. Şanlıurfa
  • Doğan-Daş, B. & Denek, N. (2021). Effect of wheat straw and molasses supplementation on quality, lamb performance, and digestibility of forage turnip (Brassica rapa) silage. Iranian Journal of Applied Animal Science, 11(3), 547-555
  • Filya, İ. (2002). The effects of lactic acid bacteria and lactic acid bacteria-enzyme mixture silage inoculants on maize silage. Turkish Journal of Veterinary and Animal Sciences, 26;679-687
  • İke, F. (2019). The effects of lactic acid bacteria and enzyme additive on the fermentation with aerobic stability of common vetch, wheat, oat mixture silages. MSc. Thesis. Tekirdağ Namık Kemal University, Graduate School of Natural and Applied Science. Tekirdağ
  • Ishler, V., Heinrichs, J. & Varga, G. (2016). From feed to milk: Understanding rumen function, Penn State Uni. College of Agricultural Sci. Extension Circular. 422, USA.
  • Kılıc, U. & Erisek, A. (2019). Effects of additive use on silage quality and in vitro digestibility of some brassica silages. Journal of Scientific and Engineering Research, 6(11):163-171
  • Kılıç, Ü. (2009). Using canola forages as roughage source in ruminant nutrition. Journal of Lalahan Livestock Research Institute, 49 (2): 125-135.
  • Kılıç, A. (1986). Silo Yemi. Bilgehan Basımevi. İzmir.
  • King, G. (2022). Animal Nutrition. Animal and Poultry Science, University of Guelph. Available at: https://animalbiosciences.uoguelph.ca/~gking/Ag_2350/nutrition. htm (Accessed NOV 25, 2021).
  • Kurşun, Z. (2009). The effects of bacterial inoculants on the fermentation, aerobic stability and in vitro organic matter digestibility characteristics of triticale silages. MSc. Thesis Namık Kemal University, Graduate School of Natural and Applied Sciences, Tekirdağ
  • NRC. (2021). Nutrient requirements of dairy cattle: eighth revised edition. Washington, DC: The National Academies Press. 502 pages. Available at: https://doi.org/10.17226/25806 (Accessed SEP 05, 2021).
  • Nursoy, H. & Şahin, E. (2017). Son metodlara göre yemlerin kuru madde analizleri. Tr. Doğa ve Fen Derg. − Tr. J. Nature Sci. 2017 Vol. 6 No. 1
  • Özkan, F. (2019). Determining the quality of sugar beet pulp, lenox and ryegrass silages used in feeding dairy cattle, in comparison with corn silage, Ph.D. Thesis, Van Yüzüncü Yıl University, Van, Turkey
  • Suzuki, M. &, Lund, C.W. (1980). Improved gas-liquid chromatography for simultaneous determination of volatile fatty acids and lactic acid in silage. Journal of Agricultural and Food Chemistry, 28, 1040-1
  • Türk, M., Albayrak, S., Balabanlı, C. & Yüksel, O. (2009). Effects of fertilization on root and leaf yields and quality of forage turnip (Brassica rapa L.). Journal of Food, Agriculture and Environment, 7(3-4): 339-342.
  • Van-Soest, P.J., Robertson, J.B. & Lewis, B.A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10): 3583-3597. doi:10.3168/jds.S0022-0302(91)78551-2.
  • Yaylak, E. & Alçiçek, A. (2003). Sığır besiciliğinde ucuz bir kaba yem kaynağı: mısır silajı. Hayvansal Üretim. 44(2), 29-36.
  • Yıldız, S., Deniz, S., Özkan, F. & Kale, Ç. (2022). Forage turnip (Brassica rapa) harvested in different phases of vegetative stage and ensiled with the additives of molasses and barley and the effects of additives on silage quality, in vitro digestibility, and energy content. Turkish Journal of Veterinary & Animal Sciences 46 (3), 475-482 DOI: 10.55730/1300-0128.4196
  • Yıldız, S., Deniz, S., Özkan, F. & Kale, Ç. (2023). Effects of ensiling forage turnip (Brassica Rapa) plant harvested in different vegetation periods with triticale (X Triticosecale Wittmack) forage at different ratios on silage quality in vitro digestibility and energy content. Journal of Animal and Plant Science, 33 (4). 855-863 https://doi.org/10.36899/JAPS.2023.4.0678
There are 27 citations in total.

Details

Primary Language English
Subjects Animal Feeding
Journal Section Araştırma Makaleleri
Authors

Serhat Yıldız 0000-0003-1063-4704

Early Pub Date September 23, 2025
Publication Date September 24, 2025
Submission Date May 6, 2025
Acceptance Date August 13, 2025
Published in Issue Year 2025 Volume: 29 Issue: 3

Cite

APA Yıldız, S. (2025). Determination of the silage quality of Lenox (Brassica rapa) product harvested in different periods. Harran Tarım Ve Gıda Bilimleri Dergisi, 29(3), 506-515. https://doi.org/10.29050/harranziraat.1693247

Indexing and Abstracting 

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10749  Harran Journal of Agricultural and Food Science is licensed under Creative Commons 4.0 International License.