Identification and Characterization of Carnitine Palmitoyltransferase 1A (CPT1A) Gene in European Sea Bass (Dicentrarchus labrax)

Year 2023, Volume: 64 Issue: 1, 6 - 11, 30.10.2023

Emel Özcan Gökçek , Raziye Işık

https://doi.org/10.29185/hayuretim.1261236

Abstract

Objective: CPT1A intervenes in the access of the long-chain fatty acyl-CoA to the mitochondrial matrix. In this study, we conducted the genetic polymorphisms of the partial sequence of the CPT1A gene in European sea bass (Dicentrarchus labrax) that were cage cultured in the Aegean Sea.
Material and Methods: Genomic DNA was isolated from 100 European sea bass individuals. After amplifying a partial region of the CPT1A gene by thermal cycler, PCR products were sequenced via Sanger method.
Results: We detected two SNPs in partial sequence of the CPT1A gene in European sea bass. TT, TA and AA genotypes were observed for CPT1A g.2080T>A locus with frequencies of 27.04, 49.92 and 23.04%, respectively. The frequencies of the g.2216A>G locus as AA, AG and GG were found to be 1.2, 19.58 and 79.21%, respectively. The CPT1A g.2080T>A locus was in HWE, whereas the g.2216A>G locus was not in HWE.
Conclusion: European sea bass has a high commercial value. These findings suggest that two SNPs in the CPT1A gene could be used for genomic selection programs related to fatty acid composition in European sea bass.
Keywords: Fatty acid, SNP, sea bass, variation

Keywords

fatty acid, SNP, sea bass, variation

Avrupa Levreğinde (Dicentrarchus labrax) Karnitin Palmitoiltransferaz 1A (CPT1A) Geni Tanımlanması ve Karakterizasyonu

Abstract

Amaç: CPT1A, uzun zincirli yağ asil-CoA'nın mitokondriyal matrise girişine müdahale eder. Bu çalışmada, Akdeniz’de kafes kültürü yapılan Avrupa levreklerinde (Dicentrarchus labrax) CPT1A geninin kısmi dizisinde genetik varyasyonlar araştırılmıştır.
Materyal ve Yöntemler: 100 Avrupa levreği bireyinde genomik DNA izole edilmiştir. CPT1A geninin kısmi bir bölgesi termal döngüleyici ile çoğaltıldıktan sonra, PCR ürünleri Sanger yöntemi ile dizilenmiştir.
Bulgular: Avrupa levreği CPT1A geninde kısmi dizisinde iki SNP belirlenmiştir. g.2080T>A lokusu için sırasıyla %27.04, %49.92 ve %23.04 frekansları ile TT, TA ve AA genotipleri gözlenmiştir. g.2216A>G lokusunun AA, AG ve GG’nin frekansları sırasıyla %1.2, %19.58 ve %79.21 olarak bulunmuştur.
Sonuç: Avrupa levreği yüksek ticari değere sahiptir. Bu bulgular, CPT1A genindeki iki SNP'nin, Avrupa levreğindeki yağ asidi bileşimi ile ilgili genomik seleksiyon programları için kullanılabileceğini düşündürmektedir.
Anahtar Kelimeler: Yağ asidi, SNP, levrek, varyasyon

Keywords

yağ asidi, SNP, levrek, varyasyon

References

• Bennett MJ, Boriack RL, Narayan S, Rutledge SL, Raff ML. 2004. Novel mutations in CPT 1A define molecular heterogeneity of hepatic carnitine palmitoyltransferase I deficiency. Molecular Genetics and Metabolism 82(1) 59–63.
• Bermejo-Nogales A, Nederlof M, Benedito-Palos L, Ballester-Lozano GF, Folkedal O, Olsen RE, Pérez-Sánchez J. 2014. Metabolic and transcriptional responses of gilthead sea bream (Sparus aurata L.) to environmental stress: new insights in fish mitochondrial phenotyping. General and Comparative Endocrinology 205: 305-315.
• Bouchard-Mercier A, Rudkowska I, Lemieux S, Couture P, Vohl MC. 2014. Polymorphisms in genes involved in fatty acid β-oxidation interact with dietary fat intakes to modulate the plasma TG response to a fish oil supplementation. Nutrients 6(3): 1145-1163.
• Cai L, Liu G, Wei Y, Zhu Y, Li J, Miao Z, Huang R. 2021. Whole-genome sequencing reveals sex determination and liver high-fat storage mechanisms of yellowstripe goby (Mugilogobius chulae). Communications Biology 4(1): 15.
• Chen Y, Sun Z, Liang Z, Xie Y, Su J, Luo Q, Wang A. 2020. Effects of dietary fish oil replacement by soybean oil and L-carnitine supplementation on growth performance, fatty acid composition, lipid metabolism and liver health of juvenile largemouth bass, Micropterus salmoides. Aquaculture 516: 734596.
• Eaton S. 2002. Control of mitochondrial β-oxidation flux. Progress in Lipid Research 41: 197–239.
• Figueiredo-Silva AC, Kaushik, S, Terrier F, Schrama J W, Médale F, Geurden I. 2012. Link between lipid metabolism and voluntary food intake in rainbow trout fed coconut oil rich in medium-chain TAG. British Journal of Nutrition 107(11): 1714-1725.
• Gan, Y., Yu, F, Fang, H. 2021. Novel mutation in carnitine palmitoyltransferase 1A detected through newborn screening for a presymptomatic case in China: a case report. Italian Journal of Pediatrics 47: 154.
• Guo X, Liang XF, Fang L, Yuan X, Zhou Y, He S, Shen D. 2015. Effects of lipid-lowering pharmaceutical clofibrate on lipid and lipoprotein metabolism of grass carp (Ctenopharyngodon idellal Val.) fed with the high non-protein energy diets. Fish Physiology and Biochemistry 41: 331–343.
• Gutiéres S, Damon M, Panserat S, Kaushik,S, Médale F. 2003. Cloning and tissue distribution of a carnitine palmitoyltransferase I gene in rainbow trout (Oncorhynchus mykiss). Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 135: 139-151.
• Gutierrez AP, Yáñez JM, Fukui S, Swift B, Davidson WS. 2015. Genome-wide association study (GWAS) for growth rate and age at sexual maturation in Atlantic salmon (Salmo salar). PLoS One 10: e0119730.
• Horn SS, Meuwissen T H, Moghadam H, Hillestad B, Sonesson AK. 2020. Accuracy of selection for omega-3 fatty acid content in Atlantic salmon fillets. Aquaculture 519: 734767.
• Hu X, Li C, Shi L. 2013. A novel 79-bp insertion/deletion polymorphism in 3′-flanking region of IGF-I gene is associated with growth-related traits in common carp (Cyprinus carpio L.). Aquaculture Research 44: 1632–1638.
• Ji K, Liang H, Ren M, Ge X, Pan L, Yu H. 2021. Nutrient metabolism in the liver and muscle of juvenile blunt snout bream (Megalobrama amblycephala) in response to dietary methionine levels. Scientific Reports 11(1): 1-13.
• Kuhl H, Beck A, Wozniak G, Canario A, Volckaert F, Reinhardt R. 2010. The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing. BMC Genomics 11: 68.
• Liu K, Liu H. Chi S, Dong X, Yang Q, Tan B. 2018. Effects of different dietary lipid sources on growth performance, body composition and lipid metabolism‐related enzymes and genes of juvenile golden pompano, Trachinotus ovatus. Aquaculture Research 49(2): 717-725.
• Louro B, Kuhl H, Tine M, de Koning DJ, Batargias C, Volckaert FAM, Reinhardt R, Canario AVM, Power DM. 2016. Characterization and refinement of growth related quantitative trait loci in European sea bass (Dicentrarchus labrax) using a comparative approach. Aquaculture 455: 8-21.
• Lu KL, Zhang DD, Wang LN, Xu WN, Liu, WB. 2016. Molecular characterization of carnitine palmitoyl-transferase IA in Megalobrama amblycephala and effects on its expression of feeding status and dietary lipid and berberine. Comparative biochemistry and physiology. Part B, Biochemistry & Molecular Biology 191: 20-25.
• Morash AJ, Kajimura M, McClelland GB. 2008. Intertissue regulation of carnitine palmitoyltransferase I (CPTI): mitochondrial membrane properties and gene expression in rainbow trout (Oncorhynchus mykiss). Biochimica et Biophysica Acta 1778: 1382-1389.
• Morash AJ, Le Moine CM, McClelland GB. 2010. Genome duplication events have led to a diversification in the CPT I gene family in fish. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 299: R579-R589.
• Özcan Gökçek E, Işık R, Karahan B, Gamsız K. 2020. Genetic variation of Insulin-like growth factor II (IGF-II) gene and its associations with growth traits in European sea bass (Dicentrarchus labrax), Turkish Journal of Fisheries and Aquatic Sciences 20(7): 541-548.
• Özcan Gökçek E, Işık R. 2020. Associations between genetic variants of the insulin-like growth factor I (IGF-I) gene and growth traits in European sea bass (Dicentrarchus labrax, L.). Fish Physiology and Biochemistry 46: 1131–1138.
• Pagani F, Baralle FE. 2004. Genomic variants in exons and introns: identifying the splicing spoilers. Nature Reviews Genetics 5(5): 389-396.
• Rimoldi S, Benedito-Palos L, Terova G, Pérez-Sánchez J. 2016. Wide-targeted gene expression infers tissue-specific molecular signatures of lipid metabolism in fed and fasted fish. Reviews in Fish Biology and Fisheries 26: 93-108.
• Shi XC, Sun J, Yang Z, Li XX, Ji H, Li Y. 2017. Molecular characterization and nutritional regulation of carnitine palmitoyltransferase (CPT) family in grass carp (Ctenopharyngodon idellus). Comparative biochemistry and physiology. Part B, Biochemistry & Molecular Biology 203:11–19.
• Sun CF, Sun HL, Dong JJ, Tian YY, Hu J, Ye X. 2019. Correlation analysis of mandarin fish (Siniperca chuatsi) growth hormone gene polymorphisms and growth traits. Journal of Genetics 98(2): 1-10.
• Xie M, Hao, Q, Olsen RE, Ringø E, Yang Y, Zhang Z, Zhou Z. 2022. Growth performance, hepatic enzymes, and gut health status of common carp (Cyprinus carpio) in response to dietary Cetobacterium somerae fermentation product. Aquaculture Reports 23: 101046.
• Yang Y, Lan Z, Shu H, Zhou H, Jiang X, Hou L, Gu P. 2018. Association between expression levels and growth trait-related SNPs located in promoters of the MC4R and MSTN genes in Spinibarbus hollandi. Genes and genomics 40(11): 1119-1125.
• Yuan Y, Jin M, Fang F, Tocher DR, Betancor MB, Jiao L, Zhou QC. 2022. New insight into the molting and growth in crustaceans: Regulation of energy homeostasis through the lipid nutrition. Frontiers in Marine Science 9: 914590.
• Zhang H, Wen JJ, Zhang YN, Limbu SM, Du ZY, Qin J G, Chen LQ. 2019. Forskolin reduces fat accumulation in Nile tilapia (Oreochromis niloticus) through stimulating lipolysis and beta-oxidation. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 230: 7-15.
• Zhang S, Zhong L, Qin Q, Wang M, Pan J, Chen X, Bian W. 2016. Three SNPs polymorphism of growth hormone-releasing hormone gene (GHRH) and association analysis with growth traits in channel catfish. Acta Microbiologica Sinica 40(5): 886-893.
• Zheng JL, Luo Z, Zhu QL, Chen QL, Gong Y. 2013. Molecular characterization, tissue distribution and kinetic analysis of carnitine palmitoyltransferase I in juvenile yellow catfish Pelteobagrus fulvidraco. Genomics. 101(3): 195–203.
• Zheng WX, Qian YX, Tong LJ. 2012. Molecular cloning and tissue distribution of carnitine palmitoyltransferases I gene from Lateolabrax japonicus. Journal of Biology 29(3): 1-5.
• Zou C, Su N, Wu J, Xu M, Sun Z, Liu Q., Ye C. 2019. Dietary Radix Bupleuri extracts improves hepatic lipid accumulation and immune response of hybrid grouper (Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀). Fish & Shellfish Immunology 88: 496-507.