A Study on Clonal Propagation by Cutting of Native Boxwood Species Buxus balearica

Year 2025, Volume: 42 Issue: 1, 22 - 32

Ömer Sarı , Fisun Gürsel Çelikel

Abstract

The aim of the study was to determine the reproductive parameters of the endangered Buxus balearica species, which is naturally distributed in Türkiye, under in vivo conditions. The effect of 0, 2000, 4000, and 8000 ppm doses of indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of semi-wood cuttings of B. balearica was investigated. As a result, it was the 4000 ppm IAA application that increased the root length (5.4 cm) and root width (5.6 cm) the most compared to the control. In both hormone applications, 8000 ppm was the most effective application on root number (6 per plant) and root quality (3.1 and 3.0). In addition, the most effective application on shoot length (1.8 cm) and the number of leaves on the shoot (7.5 per shoot) was found to be 8000 ppm IBA application. Regarding the callus rate, 4000 ppm (21%) was the most effective application in both hormone applications. In terms of rooting rate, 8000 ppm IBA provided a 98.5% higher rooting rate compared to the control. Although hormone applications had different effects on root morphologies, IBA showed a better performance than IAA on rooting rate. Additionally, phytohormone applications had a positive effect on rooting. The data obtained can be used to propagate existing boxwoods in landscape areas and for the rehabilitation of disappearing forests.

Keywords

Callus rate, Indole-3-acetic acid, Indole-3-butyric acid, In vivo, Rooting rate

References

• Ağaoğlu, Y.S., Çelik, H., Çelik, M., Fidan, Y., Gülşen, Y., Günay, A., Halloran, N., Köksal, A.İ., & Yanmaz, R. (2019). Genel bahçe bitkileri. Ankara Üniversitesi Basım Evi, Ankara, 319 s. (İn Turkish).
• Ait-Mohamed, O., Battisti, V., Joliot, V., Fritsch, L., Pontis, J., Medjkan, S., & Ait-Si-Ali, S. (2011). Acetonic extract of Buxus sempervirens induces cell cycle arrest, apoptosis and autophagy in breast cancer cells. PloS one, 6(9): e24537.
• Ak, K., Sarı, Ö., Altaş, K., & Yaşar, H. (2021). A new pest in Hatay province boxwood areas, Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae). Artvin Coruh University Journal of Forestry Faculty, 22(1): 109-116.
• Akhtar, G., Akram, A., Sajjad, Y., Balal, R.M., Shahid, M.A., Sardar, H., Naseem, K.., & Shah, S.M. (2015). Potential of plant growth regulators on modulating rooting of Rosa centifolia. American Journal of Plant Sciences, 6(5): 659.
• Al-Zebari, S.M.K., & Al-Brifkany, A. (2015). Effect of cutting type and IBA on rooting and growth of citron (Citrus medica L). American Journal of Experimental Agriculture, 5(2): 134-138.
• Azad, M.S., & Matin, M.A. (2015). Effect of indole‐3‐butyric acid on clonal propagation of swietenia macrophylla through branch cutting. Journal of Botany, 1: 249308.
• Balta, M.F., Erol, İ.U., Özrenk, K., Karakaya, O., & Uzun, S. (2019). Investigation on propagation with softwood cuttings of cornelian cherry (Cornus mas L.) genotypes. Türkiye Journal of Agriultural Research, 6(2): 136-141.
• Banko, T.J., & Stefani, M.A. (1986). Effects of wounding, IBA and basal trimming on rooting of boxwood cuttings. Journal of Environmental Horticulture, 4(3): 72-73.
• Batdorf, L.R. (1997). Boxwood handbook: a practical guide to knowing and growing boxwood. 3rd edition. Boyce, VA, USA: The American Boxwood Society.
• Batdorf, L.R. (2004). Boxwood; an Illustrated Encyclopedia. The American Boxwood Society, Boyce VA. 343 pp.
• Benkhnigue, O., Akka, F.B., Salhi, S., Fadli, M., Douira, A., & Zidane, L. (2014). Catalogue des plantes médicinales utilisées dans le traitement du diabète dans la région d’Al Haouz-Rhamna (Maroc). Journal of Animal and Plant Sciences, 3: 3539–3568.
• Billen, W. (2007). Diaphania perspectalis (Lepidoptera: Pyrallidae)-anew moth in Europe. Mitteilungen der Entomologischen Gesellschaft Basel, 57(2/4): 135-137.
• Çelik, H. (1982). Kalecik Karası /41 B Aşı Kombinasyonu için Sera Koşullarında Yapılan Aşılı Köklü Fidan Üretiminde Değişik Köklenme Ortamları ve NAA Uygulamalarının Etkileri. PhD Thesis, Ankara University, 73 s., (in Turkish).
• Dhakal, K., Bika, R., Ghimire, B., Parajuli, M., Neupane, S., Neupane, K., Addesso, K.M., & Baysal-Gurel, F. (2022). Arthropod and disease management in boxwood production. Journal of Integrated Pest Management, 13(1): 1-20.
• Decocq, G., Bordier, D., Wattez, J.R., & Racinet, P. (2004). A Practical Approach to assess the native status of a rare plant species: The controverse of Buxus sempervirens L. in northern france revisited. Plant Ccology, 173(1): 139-151.
• Davies, P.J. (2010). The plant hormones: their nature, occurrence, and functions. In Plant hormones: biosynthesis, signal transduction, action (pp. 1-15). Dordrecht: Springer Netherlands.
• Göktürk, T. (2017). The effect of dipel and spruzit against Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae), International forestry and environment symposium "Climate Change and Tree Migration" 7-10 November 2017, Abstract Book, p. 125, Trabzon, Türkiye.
• Güney, D., Chavoshi, S.H., Bayraktar, A., & Atar, F. (2021). The effects of temperature and exogenous auxin on cutting propagation of some junipers. Dendrobiology, 86: 29-38.
• Güney, D., Bayraktar, A., Atar, F., Chavoshi, S.H., & Turna, İ. (2023). The effects of different rooting temperatures and phytohormones on the propagation of boxwood cuttings. Baltic Forestry, 29(1): id593.
• Hartman, H.T. (2002). Plant Propagation: Principles and Practices. 6th Edn., Prentice-Hall, India, ISBN-10: 0132061031, pp: 770.
• Hartmann, H.T., Kester, D.E., Davies, F.T., & Geneve, R.L. (2011). Plant propagation, principles and practices. 8th Ed., Prentice Hall Inc., New Jersey, 880 pp.
• Hizal, E., Kose, M., Yesil, C., & Kaynar, D. (2012). The new pest Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae) in Türkiye. Journal of Animal and Veterinary Advances, 11(3): 400-403.
• Kalyoncu, İ., Ersoy, N., & Aydın, M. (2008). The effects of some hormone and humidity levels on rooting of Mahaleb (Prunus mahaleb L.) softwood top cuttings. Süleyman Demirel University Journal of Agriculture Faculty, 3(1): 32-41.
• Kaushik, S., & Shukla, N. (2020). A review on effect of IBA and NAA and their combination on the rooting of stem cuttings of different ornamental crops. Journal of Pharmacognosy and Phytochemistry, 9(3): 1881-1885.
• Kaviani, B., & Negahdar, N. (2017). Propagation, micropropagation and cryopreservation of Buxus hyrcana Pojrk., an endangered ornamental shrub. South African Journal of Botany, 111: 326-335.
• Kaygın, T.A., & Taşdeler, C. (2018). Cydalima perspectalis (Walker) (Lepidoptera: Crambidae) Şimşirlerimiz için bir tehdit mi? III. Türkiye Orman Entomolojisi ve Patolojisi Sempozyumu, Bildiri Özetleri Kitabı, s: 21, 10-12 Mayıs 2018, Artvin (in Turkish). Köhler, E. (2007). Buxaceae. In Flowering Plants· Eudicots Springer, Berlin, Heidelberg, pp. 40-47.
• Kramer, M., Guo, Y., & Pooler, M. (2020). Ranking resistance of Buxus cultivars to boxwood blight–an integrated analysis. Journal of Environmental Horticulture, 38(2): 50-55.
• Langé, P.P. (2014). Efecto de auxinas en el enraizamiento de estaquillas de Buxus sempervirens L, En Distintas Épocas Del Año.
• Larson, P.D. (1996). Boxwood: İts History, Cultivation, Propagation and Descriptions. Boyce, VA: Foliar Press VI.
• Larson, P.D. (1999). Boxwood: Its history, cultivation, propagation and descriptions. Boyce, VI: Foliar Press.
• Lázaro, A., & Traveset, A. (2005). Spatio‐temporal variation in the pollination mode of Buxus balearica (Buxaceae), an ambophilous and selfing species: mainland‐island comparison. Ecography, 28(5): 640-652.
• Mitchell, R., Chitanava, S., Dbar, R., Kramarets, V., Lehtijärvi, A., Matchutadze, I., Mamadashvili, G., Matsiakh, I., Nacambo, S., Papazova-Anakieva, I., Sathyapala, S., Tuniyev, B., Vétek, G., Zukhbaia, M., & Kenis, M. (2018). Identifying the ecological and societal consequences of a decline in Buxus forests in Europe and the Caucasus. Biological Invasions, 20: 3605-3620.
• Muhammad Shahab, A. (2021). Effect of IBA (Indole butyric acid) levels on the growth and rooting of different cutting types of Clerodendrum splendens. Pure and Applied Biology (PAB), 5(1): 64-71.
• Öztürk, N., Akbulut, S., & Yüksel, B. (2016). Düzce için yeni bir zararlı Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae), Düzce Üniversitesi Ormancılık Dergisi, 12(1): 112- 121 (in Turkish).
• Pulatkan, M., Yıldırım, N., & Şahin, E.K. (2018). Effect of different hormone doses on rooting of Berberis thunbergii “Atropurpurea Nana” cuttings Turkish Journal of Forestry, 19(4): 386-390.
• Salaš, P., Sasková, H., Mokričková, J., & Litschmann, T. (2012). Evaluation of different types of rooting stimulators. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 26(8): 217.
• Sarı, Ö., Çelikel, F.G., & Yaşar, H. (2022). Current status and the last locations of Türkiye’s native Buxus species (Buxus sempervirens L. and Buxus balearica Lam.) under threats. International Journal of Agricultural and Wildlife Sciences, 8(2): 179-196.
• Sarı, Ö., Çelikel, F.G., & Yaşar, H. (2023). Türkiye's natural boxwood (Buxus sempervirens and Buxus balearica) locations. International Journal of Agricultural and Wildlife Sciences, 9(3): 311-333.
• Savill, P. (2013). Buxus sempervirens L.-box. In: The silviculture of trees used in British forestry. CABI Publishing, CABI, pp 48–51.
• Singh, K.K., Krishan, C., & Singh, K. (2018). Propagation of citrus species through cutting: A review. Journal of Medicinal Plants Studies, 6(1): 167-172.
• Tosun, F., Kızılay, Ç., Şener, B., Vural, M., & Palittapongarnpim, P. (2004). Antimycobacterial activity of some Turkish plants. Pharmaceutical biology, 42(1): 39-43.
• Tuniyev, B.S. (Ed.) (2016). Colchis boxwood—Buxus colchica: retrospective and current status of populations (monograph). In: Proceeding of the Sochi National Park, 7. Publ. buki vedi, Moscow.
• Tutin, T.G., Heywood, V.H., Burges, N.A., Moore, D.M., Valentine, D.H., Walters, S.M., & Webb, D.A. (1968). Flora Europaea, Rosaceae to umbelliferae, Cambridge University Press, Cambridge, vol. 2.
• Van der Straten, M.J., & Muus, T.S. (2010). The box tree pyralid (Glyphodes perspectalis (Walker 1859), Lepidoptera: Crambidae); an invasive alien moth ruining box trees. In Proceedings of the Netherlands. Entomological Society Meeting, 21: 107-111
• Van Trier, H., Hermans, D., Theunynck, A., & Dumon, M. (2005). Buxus. Stichting kunstboek.
• Van Trier, H., & Hermans, D. (2007). Buchs. Eugen Ulmer, Stuttgart.
• Vieira, L.M., Kruchelski, S., Gomes, E.N., & Zuffellato-Ribas, K.C. (2018). Indolebutyric acid on boxwood propagation by stem cuttings. Ornamental Horticulture, 24(4): 347-352.
• Walter, V. (1997). Rozmnožování okrasných stromů a keřů. Praha: Brázda, 310 s. ISBN 80-209- 0268-6.
• Wang, Y.T. (1989). Medium and hydrogel affect production and wilting of tropical ornamental plants. HortScience, 24: 941-944.
• Yavuz, Ç. (2015). Malatya ilinden selekte edilen bazı kızılcık (Cornus mas L.) genotiplerinde farklı IBA uygulamalarının yeşil çeliklerin köklenmesi üzerine etkileri. MSc Thesis, Kahramanmaraş Sütçü İmam University, 37 p. Türkiye.
• Yeshiwas, T., Alemayehu, M., & Alemayehu, G. (2015). Effects of indole butyric acid (IBA) and stem cuttings on growth of stenting-propagated rose in Bahir Dar, Ethiopia. World Journal of Agricultural Sciences, 11(4): 191-197.
• Yıldız, K., Çekiç, Ç., Güneş, M., & Özgen, M. (2009). The determination of rooting success of different types of black mulberry (Morus nigra L.) cuttings. Journal of Agricultural Faculty of Gaziosmanpaşa University, 26(1): 1-5.
• Yıldız, Y., Yıldırım, İ., & Bostancı, C. (2018). Bartın ilinin istilacı böcek türleri, III. Türkiye Orman Entomolojisi ve Patolojisi Sempozyumu, s. 25, 10-12.05.2018, Artvin, Türkiye (in Turkish).