Preparation of Metformin HCl-loaded Chitosan Microspheres and In vitro Characterization Studies

Year 2015, Issue: 1, 74 - 86, 01.01.2015

Behzad Mokhtare Meltem Cetin Rukiye Sevinc Ozakar Hatice Bayrakceken

Abstract

This study focuses on the preparation and in vitro characterisation of metformin HCl-loaded chitosan CS microspheres MS . Scanning Electron Microscopy SEM image shows that the microspheres have spherical shape. The particle size and encapsulation efficiency % value of the metformin HCl-loaded microspheres were found as 9.21±2.16 µm and 20.05±6.92%, respectively. The obtained low drug loading value is due to the high aqueous solubility of metformin HCl, and drug leakage from microspheres to the aqueous medium. In HCl pH 1.2 and phosphate buffer PB pH 6.8 mediums, about 22-36% of metformin HCL was released within 1 hour and about 89-98% of the loaded metformin HCl was released in 8 days. Furthermore, microspheres were characterised using FT-IR and DSC. These data demonstrate a potential for sustained release of metformin HCl by using prepared CS microspheres.

Keywords

Chitosan, DSC, FT-IR, Metformin HCl, Microsphere

Metformin HCl-yüklü Kitosan Mikrokürelerinin Hazırlanması ve In vitro Karakterizasyon Çalışmaları

Abstract

Bu çalışma, metformin HCl-yüklü kitosan CS mikrokürelerinin MS hazırlanması ve in vitro karakterizasyonu üzerine odaklanmıştır. Taramalı Elektron Mikroskobu SEM görüntüsü küresel mikrokürelerin hazırlandığını göstermiştir. Metformin HCl-yüklü mikrokürelerin partikül büyüklüğü ve enkapsülasyon etkinliği % değerleri sırasıyla 9.21±2.16 µm ve %20.05±6.92 olarak bulunmuştur. Metformin HCl’nin sudaki yüksek çözünürlüğü ve etkin maddenin sulu ortama kaçışı düşük yükleme etkinliği değeri elde edilmesine neden olmuştur. HCl pH 1.2 ve fosfat tamponu PB pH 6.8 ortamlarında yaklaşık %22-36 metformin HCl salımı 1 saat içerisinde ve yaklaşık %89-98 metformin HCl salımı ise 8 gün içerisinde gerçekleşmiştir. Ayrıca, FT-IR ve DSC mikrokürelerin karakterizasyonu için kullanılmıştır. Bu veriler, metformin HCl’nin sürekli salımı için CS mikrokürelerinin potansiyelini göstermektedir.

Keywords

DSC, FT-IR, Kitosan, Metformin HCl, Mikroküre

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