Yüksek Konsantrasyonlardaki Gadobutrol Hücre Canlılığını ve Genom Bütünlüğünü Tehlikeye Atıyor: Klinik Görüntülemede İhtiyatlı Kullanım İçin Bir Çağrı

Abstract

Amaç: Gadobutrol, gadolinyum türevi olarak geliştirilmiş paramanyetik ve noniyonik bir kon-trast ajandır. Bu ajan rutin olarak tanısal amaçlı kullanılmaktadır. Ayrıca doku kontrastını arttırdığı için rezonans anjiyografi ve manyetik rezonans görüntülemede (MR) tercih edilmektedir. Bu çalışmanın amacı gadobutrolün NIH3T3 hücre hattı üzerindeki in vitro si-totoksik ve genotoksik etkilerini belirlemektir. Materyal ve Metod: NIH3T3 hücre hattı standart koşullar altında (37°C, %5 CO₂) büyütüldü. Sitotoksik etkiyi belirlemek için CCK-8 kiti kullanılmıştır. Genotoksisite potansiyeli Comet testi ile belirlendi. Bulgular: Gadobutrol düşük dozlarda hücre canlılığı üzerinde anlamlı bir etkiye sahip değildi, ancak konsantrasyon arttıkça (10-100 mM) hücre canlılığı istatistiksel olarak anlamlı bir şekil-de azaldı (p<0.0001). Sonuç: Gadobutrol yüksek konsantrasyonlarda NIH3T3 hücrelerinde belirgin sitotoksik ve geno-toksik etkiler göstermiştir.

Keywords

• Sitotoksisite
• Genotoksisite
• Gadobutrol
• NIH3T3
• Comet Assay

Gadobutrol at High Concentrations Compromises Cell Viability and Genome Integrity: A Call for Prudent Use in Clinical Imaging

Abstract

Background: Gadobutrol is a paramagnetic and nonionic contrast agent developed as a gado-linium derivative. This agent is routinely used for diagnostic purposes. It is also preferred in resonance angiography and magnetic resonance imaging (MR) because it increases tissue contrast. The aim of this study was to determine the in vitro cytotoxic and genotoxic effects of gadobutrol on NIH3T3 cell line. Materials and Methods: NIH3T3 cell line was grown under standard conditions (37°C, 5% CO₂). CCK-8 kit was used to determine the cytotoxic effect. Genotoxicity potential was deter-mined by Comet assay. Results: Gadobutrol had no significant effect on cell viability at low concentrations, but as the concentration increased (10-100 mM), cell viability decreased statistically significantly (p<0.0001). Conclusions: Gadobutrol showed marked cytotoxic and genotoxic effects in NIH3T3 cells at high concentrations.

Keywords

• Cytotoxicity
• Genotoxicity
• Gadobutrol
• NIH3T3
• Comet Assay

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