Kemik Defekti Oluşturulan Ratlarda Chitosan Acetate’ın Erken Kemik İyileşmesi Üzerine Etkisi

Abstract

Amaç:Kırık iyileşmesi, oldukça karmaşık patofizyolojik olayları içermesi ve sağaltımında multidisipliner yaklaşımlara gerek olmasından dolayı araştırmacıların üzerinde birçok çalışma yaptıkları bir alandır. Kırık iyileşmesini olumlu etkileyen ajanların bir kısmı klinikte kullanım alanı bulmuştur. Kemik iyileşmesinde kullanılan ideal materyalin kemik onarımı üzerine etkili, uygulanması kolay nitelikleri bulunmalıdır. Çalışmamızda kemik defekti oluşturulan ratlarda Chitosan Acetate Bandage’nin kemik iyileşmesi üzerine etkisini inceledik.

Materyal ve Metod:Tibiasında kemik defekti oluşturulan ve her biri on rat içeren iki çalışma grubu oluşturuldu. Birinci grupta; defekt serum fizyolojik ile irrige edildi ve 7. günde kemik segmenti alındı. İkinci grupta; defekte Chitosan Acetate Bandage emdirildi ve 7. günde kemik segmenti alındı. Örnekler osteoblastik aktivite, inflamatuar yanıt ve fibrozis skorları yönünden histopatolojik olarak değerlendirildi. İstatiksel analizler için ki kare testi kullanıldı. Tüm istatistikler için anlamlılık sınırı p<0.05 kabul edildi.

Bulgular:Yedinci gün değerlendirilen osteoblastik aktivite (Evre 4), inflamatuar yanıt (Evre 4) skorları Chitosan uygulanan grupta normal salin uygulanan gruba göre istatistiksel olarak anlamlı yüksek tespit edilirken, fibrozis skorları (Evre 3, 4) yönünden gruplar arasında istatistiksel olarak anlamlı farklılık gözlenmedi.

Sonuç: Kemik defekti oluşturulan ratlarda Chitosan Acetate Bandage’nin kemik iyileşmesi üzerine histopatolojik değerlendirmeler göz önüne alındığında olumlu olabileceği sonucuna varılmıştır. 

Keywords

• Chitosan Acetate,Kemik iyileşmesi,Travma

Effects of Chitosan Acetate on Early Bone Healing in The Rats That Create Bone Defect

Abstract

Aim: Bone healing is a complex process which includes pathophisiologic events that need a multidisciplinary aproach. Because of that most authors have studies on this process. The agents which effect the bone healing positively, have a clinic usage. The best material to use for bone healing have to be effected on bone repair, and easy to use. In our study, we aimed to investigate the effects of Chitosan Acetate Bandage on bone healing.

Materials and Methods:Two separate study groups were formed each one including ten rats that crate defects on their tibias. In the first group, the defect had been irrigated by serum phisiologic and the bone biopsies had taken form bone on the seventh day. In the second group, Chitosan Acetate Bandage had applied to the defect and the bone biopsies had taken form bone on the seventh day. The samples evaluated histopathologically for osteoblastic activite, inflamatory response and fibrosis scores. Ki kare test was employed to statistically analysis of the data and the significance line accepted as p<0.05.

Results:Osteoblastic activite (Stage 4) and inflamatory response (Stage 4) were statistically significant high in Chitosan group according to normal salin group. However there were no statistically significant results for fibrosis scores (Stage 3, 4) between two groups.

Conclusion:We determined positive effects of Chitosan Acetate on rats, that have bone defects by histopathologic evaluations.

Keywords

• Chitosan Acetate,Bone healing,Trauma

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