Çocuklar İçin Potansiyel Bir Tehlike: Şarbon

Abstract

Herein we report a cutaneous anthrax in a child from eastern region of Turkey. A six-year-old girl has admitted pediatric emergency clinic with a black eschar on her right thumb. The patient’s parents described an erythematous painless papule on this region starting five days ago. There was no prior history of insect bite or trauma. Her parents job was livestock farming and living in an area endemic for anthrax. The body temperature was 36.7°C, pulse rate was 105/min, blood pressure was 95/65 mm/Hg, and respiratory rate was 24/min. A 1.5x1.5 cm irregular and black skin eschar was detected on the right thumb with central necrotic appearance (Figure 1). Examination of other systems was normal. Laboratory examinations showed that the leukocyte count was 12770 mm3 and C-reactive protein level was 0.55 mg/dL (normal: 0-0.8 mg/dL). Other laboratory results were normal. Lesion swab and blood samples were taken for Bacillus anthracis culture but no bacterial growth was observed. Although blood polymerase chain reaction (PCR) was negative, PCR of the swab specimen obtained from lesion was positive for B. anthracis plasmids (pXO1 and pXO2) (Figure 2). According to the WHO guidelines, the primers PA5-PA8 for the amplification of the protective antigen broad region of plasmid pXO1 and the CAP 1234-CAP 1301 primers for amplification of the plasmid pXO2 capsu-lar region were used in the PCR reaction (4). The PCR reaction was run on a Qiagen RotorGene 6000 (Hilden Germany) Real-time instrument using Evagreen qPCR master mix (Bio-Rad Lab Inc USA). Cutane-ous anthrax

Keywords

• Şarbon,Çocuk

A Potential Danger for Children: Anthrax

Abstract

References

1. Head BM, Rubinstein E, Meyers AF. Alternative pre-approved and novel therapies for the treatment of anthrax. BMC Infect Dis 2016;16(1):621.
2. Tekin R, Sula B, Devecı O, Tekin A, Bozkurt F, Ucmak D, et al. Cutaneous anthrax in Southeast Anatolia of Turkey. Cutan Ocul Toxicol 2015;34(1):7-11.
3. Ozanich RM, Colburn HA, Victry KD, Bartholomew RA, Arce JS, Heredia-Langner A, et al. Evaluation of PCR Systems for Field Screening of Bacillus anthracis. Health Secur 2017;15(1):70-80.
4. WHO/EMC/ZDI/98.6. Guidelines for the Surveillance and Control of Anthrax in Human and Animals. 3rd edition. Principal Author: PCB Turnbull