Non-Diabetik Metabolik Sendromda İCAM-1 E469K ve E-Selektin S128R Polimorfizmleri

Year 2015, Volume: 12 Issue: 1, 58 - 66, 15.04.2015

Halit Akbas Elif Oguz Suzan Tabur Mahmut Balkan , Hakan Korkmaz Belgin Alasehirli

Abstract

Amaç: Metabolik sendrom (MetS), abdominal obezite ile ilişkili viseral yağ, insulin direnci, dislipidemi ve
hipertansiyon gibi sistemik bozuklukların bir grubudur. İnflamasyon biyomarkırları ile endotelyal
disfonksiyon, metabolik sendrom ve inflamatuar şartlarla ilişkilendirilmiştir. Son çalışmalar inflamatuar
genlerdeki genetik varyasyonların metabolik sendromda kilit rol oynadığını ileri sürmektedirler. Bu
çalışmada non-diabetik MetS li hastalarda, inflamatuar genlerden İCAM-1 ve E-selektin gen
polimorfizmlerinin araştırılması amaçlanmıştır.
Metod: Çalışmaya non-diabetik MetS'li 132 hasta ve 118 kontrol örneği dahil edilmiştir. İCAM-1 E469K
(1462A>G ;rs5498) ve E-selektin S128R (561A>C;rs5361) gen polimorfizmlerini belirlemek için polimeraz
zincir reaksiyonu- restriksiyon parça uzunluk polimorfizmi (PCR-RFLP) tekniği uygulanmıştır.

Bulgular: Kontrol grubuna kıyasla, non-diabetik MetS'li hastalarda Hem İCAM-1 E469K hemde E selektin
S128R polimorfizmlerinin genotip dağılımı ve allel frekanslarında herhangi bir değişiklik saptanmamıştır.
Sonuç: Bu çalışma, non-diabetik MetS'lu hastalarda İCAM-1 E469K ve E selektin S128R
polimorfizmlerinin araştırıldığı ilk çalışmadır. Bu çalışmadan elde edilen veriler, İCAM-1 E469K ve E
selektin S128R gen polimorfizmlerinin Türk populasyonundaki non-diabetik MetS ile ilişkili olmadığını
göstermiştir.

Keywords

Metabolik sendrom , İCAM-1 , E-Selektin , gen polimorfizmi , Polimeraz zincir reaksiyonu

ICAM-1 E469K and E-Selectin S128R Polymorphisms with Non-Diabetic Metabolic Syndrome

Abstract

Backgrounds: Metabolic syndrome (MetS) is a cluster of abdominal obesity linked to an excess of visceral
fat, insülin resistance, dyslipidemia and hypertension. Inflammation biomarkers and endothelial dysfunction
is associated with metabolic syndrome (MetS) and inflammatory condition. Recent studies suggest that
genetic variation in inflammatory genes plays a pivotal role in MetS. We aimed to investigate the
polymorphisms of two inflammatory genes, ICAM-1 and E-selectin with non-diabetic MetS in Turkish
population.
Methods: The study included 132 patients with non-diabetic MetS. and 118 control subjects. Polymerase
chain reaction-restriction fragment length polymorphism (PCR-RFLP) method were used to determine the E
selectin S128R (561A>C;rs5361) and ICAM-1 E469K (1462A>G;rs5498) polymorphisms.
Results: There was no change in genotype and allele frequencies in both ICAM-1 E469K and E selectin
S128R polymorphisms compared to control subjects.
Conclusions: The realtionship between ICAM-1 and E-selectin polymorphisms and non-diabetic MetS was
investigated firstly examined in this study. The data of this study suggest that ICAM-1 E469K and E-selectın
S128R polymorphisms are not in susceptibility to non-diabetic MetS in the Turkish population

Keywords

Metabolic syndrome , ICAM-1 , E-Selectin , gene polymorphism , Polymerase Chain Reaction

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