Evaluation of the Effects of Topical Vitamin D on Osseointegration of Titanium Implants: An Experimental Study

Year 2021, , 176 - 180, 27.08.2021

Alihan Bozoglan , Serkan Dündar

https://doi.org/10.35440/hutfd.903312

Abstract

Background: The aim of this study was to evaluate the effects of topical vitamin D to on osseointegration when applied to the surface of titanium implants.
Material and Metods: Twenty male Spraque-Dawley rats were used in this study in a 4-week experimental period. 4 rats died during the experiments. The rats were divided into 2 groups: a test group and control group. Sixteen sandblasted and acid-etched implants were integrated surgically in the metaphyseal part of rat femurs after patella dissection. Prior to integration, the implants, which were 3.2 mm in diameter and 8 mm in length, were submerged in a 10% vitamin D solution in the test group. No treatment was applied in the control group. After the 4-week experimental period, the rats were sacrificed, and the implants were removed with surrounding bone tissue. Specimens were fixed for 7 days in a 10% formaldehyde solution. Bone implant contact (BIC) ratios were determined after using non-decalcified histological methods.
Results: In the experiment, 2 samples were removed because they were not suitable. No statistically significant differences were found for BIC ratios between the test (n = 7) and control group (n = 7) implants (P> 0.05).
Conclusion: It was concluded, within the limitations of this animal study, that topical vitamin D application on implant surfaces does not affect BIC ratios.

Keywords

osseointegration, bone implant contact, topical vitamin-d, rat femoral bone

Thanks

All of the experimental and surgical protocols in our study were carried out in the Experimental Research Center of Fırat University. In addition, ethical approval permission was given by Fırat University Animal Experiments Local Ethics Committee. The animals used in the study were provided by Fırat University Experimental Research Center. The authors wish to thanks (Implants Dental Implant System, AGS Medical, Istanbul, Turkey) for manufacturing and providing the titanium implants. The information presented in this study was presented as an oral presentation at the Turkish Dental Association 24th International Congress of Dentistry between 27-30 September 2018.

Topikal D Vitamininin Titanyum İmplantların Osseointegrasyonu Üzerine Etkilerinin Değerlendirilmesi: Deneysel Bir Çalışma

Abstract

Amaç: Bu çalışmanın amacı, titanyum implantların yüzeyine uygulandığında topikal D vitamininin osseointegrasyon üzerindeki etkilerini değerlendirmektir.
Materyal ve metod: Bu çalışmada 4 haftalık bir deney periyodunda 20 erkek Spraque-Dawley sıçanı kullanıldı. 4 rat deneyler sırasında öldü. Daha sonra ratlar 2 gruba ayrıldı: Test grubu (n=8) ve kontrol grubu (n=8). Patella diseksiyonu sonrası sıçan femurlarının metafiz kısmına 16 tane kumlanmış ve asitle pürüzlendirilmiş implant cerrahi olarak entegre edildi. Entegrasyon öncesinde 3,2 mm çapında ve 8 mm uzunluğunda olan implantlar, test grubunda % 10'luk bir D vitamini çözeltisine daldırıldı. Kontrol grubuna herhangi bir tedavi uygulanmadı. 4 haftalık deney süresinden sonra, sıçanlar öldürüldü ve implantlar çevreleyen kemik dokusuyla birlikte çıkarıldı. Örnekler % 10 formaldehit solüsyonunda 7 gün fikse edildi. Kemik-implant kaynaşması (BIC) oranları, dekalsifiye edilmemiş histolojik yöntemler kullanılarak belirlendi.
Bulgular: Deneyde 2 örnek uygun olmadığı için çıkartıldı.Test (n=7) ve kontrol grubu (n=7) implantları arasında BIC oranları için istatistiksel olarak önemli farklılıklar tespit edilmedi (P> 0.05).
Sonuç: Bu hayvan çalışmasının sınırları dahilinde, implant yüzeylerine topikal D vitamini uygulamasının BIC oranlarını etkilemediği sonucuna varıldı.

Keywords

osseointegrasyon, kemik-implant kaynaşması, topikal vitami-d, sıçan femoral kemiği

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