Effect of N Acethylcystein and Methylprednisolone on Acute Pancratitis and Lung Complications in Experimental Acute Pancratitis Model

Abstract

Background: This study aims to determine N-acetyl cysteine (NAC) and methylprednisolone effects on the lung and pancreas in an experimental acute pancreatitis model.
Materials and Methods: A total of 64 male Wistar albino rats weighing 175-240 g were included in the study. Four groups of an equal number of rats (n=16) were formed. For the control group, 80 mcg/kg saline was injected into group 1. 80 mcg/kg cerulein was used to induce pancreatitis in groups 2, 3, and 4. Group 3 received NAC (1,000 mg/kg) and group 4 received methylprednisolone (5 mg/kg). Decapitation was applied to half of the groups after 7 hours (short term) and the other half after 24 hours (long term). Blood amylase and lipase values were examined. Edema in the pancreatic tissue was evaluated using the Schönberg scor-ing. Lung tissue was evaluated using scoring ranging from 0 to 3.
Results: Pancreatitis was successfully induced in all subjects in groups 2, 3, and 4. Amylase and lipase values of group 3 and group 4 were lower than group 2 in the short term (p <0.005). For short-term results, sub-jects given methylprednisolone and NAC exhibited better histopathological status in both pancreas and lungs (p = 0.001; p = 0.019, respectively). Only the pancreatic Schönberg score was significantly lower in the methylprednisolone treatment group (p = 0.0001).
Conclusions: Our study revealed that NAC and methylprednisolone have positive effects in the short term both biochemically and histopathologically in the treatment of acute pancreatitis and related respiratory complications.

Key Words: 

Keywords

• Acute pancreatit
• N-acetyl cysteine
• Methylprednisolone
• Acute pancreatit, N-acetyl cysteine, Methylprednisolone

Deneysel Akut Pankreatit Modelinde N Asetilsistein İle Metilprednisolon’un Akut Pankreatit ve Akciğer Komplikasyonları Üzerine Etkisi

Abstract

Amaç: Bu çalışma, deneysel akut pankreatitte N-asetil sisteinin (NAC) ve metilprednizolonun, akciğer ve pankreas üzerindeki etkilerini belirlemeyi amaçlamaktadır. Gereç ve Yöntemler: Çalışmaya 175-240 gr ağırlığında toplam 64 adet erkek Wistar albino sıçan dahil edildi. Eşit sayıda sıçan (n = 16) olan dört grup oluşturuldu. Kontrol grubu için grup 1'e 80 mcg/kg salin enjekte edildi. Grup 2,3 ve 4'te pankreatiti indüklemek için 80 mcg/kg serulein kullanıldı. Grup 3'e NAC (1,000 mg/kg) ve Grup 4'e metilprednizolon (5 mg/kg) verildi. Grupların yarısına 7 saat sonra (kısa dönem), diğer yarısına 24 saat sonra (uzun dönem) dekapitasyon uygulandı. Kan amilaz ve lipaz değerleri incelendi. Pankreas dokusundaki ödem, Schönberg skorlaması kullanılarak değerlendirildi. Akciğer dokusu, 0 ila 3 arasında değişen puanlama kullanılarak değerlendirildi. Bulgular: Grup 2, 3 ve 4'te tüm deneklerde pankreatit başarıyla indüklendi. Grup 3 ve grup 4'ün amilaz ve lipaz değerleri grup 2'ye göre kısa dönemde daha düşüktü (p <0,005). Kısa dönem sonuçlar için, metilprednizolon ve NAC verilen denekler hem pankreasta hem de akciğerlerde daha iyi histopatolojik durum sergiledi (sırasıyla p = 0.001; p = 0.019). Uzun dönem sonuçlar için, metilprednizolon tedavi grupunda sadece pankreas Schönberg skoru anlamlı olarak daha düşüktü (p = 0.0001). Sonuç: Çalışmamız, akut pankreatit ve buna bağlı solunumsal komplikasyonların tedavisinde NAC ve metilprednizolon hem biyokimyasal hem de histopatolojik olarak kısa vadede olumlu etkileri olduğu ortaya koymuştur.

Keywords

• acute pancreatit
• N-acetyl cystein
• metylprednisolon

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