Kemik Defekti Oluşturulan Ratlarda Chitosan Acetate’ın Erken Kemik İyileşmesi Üzerine Etkisi

Year 2018, Volume: 15 Issue: 1, 18 - 24, 22.04.2018

Ebru Güler Mustafa Burak Sayhan , Ömer Salt , Mert Çiftdemir Nuray Can

Abstract

Amaç:Kırık iyileşmesi, oldukça karmaşık patofizyolojik olayları içermesi ve sağaltımında multidisipliner yaklaşımlara gerek olmasından dolayı araştırmacıların üzerinde birçok çalışma yaptıkları bir alandır. Kırık iyileşmesini olumlu etkileyen ajanların bir kısmı klinikte kullanım alanı bulmuştur. Kemik iyileşmesinde kullanılan ideal materyalin kemik onarımı üzerine etkili, uygulanması kolay nitelikleri bulunmalıdır. Çalışmamızda kemik defekti oluşturulan ratlarda Chitosan Acetate Bandage’nin kemik iyileşmesi üzerine etkisini inceledik.

Materyal ve Metod:Tibiasında kemik defekti oluşturulan ve her biri on rat içeren iki çalışma grubu oluşturuldu. Birinci grupta; defekt serum fizyolojik ile irrige edildi ve 7. günde kemik segmenti alındı. İkinci grupta; defekte Chitosan Acetate Bandage emdirildi ve 7. günde kemik segmenti alındı. Örnekler osteoblastik aktivite, inflamatuar yanıt ve fibrozis skorları yönünden histopatolojik olarak değerlendirildi. İstatiksel analizler için ki kare testi kullanıldı. Tüm istatistikler için anlamlılık sınırı p<0.05 kabul edildi.

Bulgular:Yedinci gün değerlendirilen osteoblastik aktivite (Evre 4), inflamatuar yanıt (Evre 4) skorları Chitosan uygulanan grupta normal salin uygulanan gruba göre istatistiksel olarak anlamlı yüksek tespit edilirken, fibrozis skorları (Evre 3, 4) yönünden gruplar arasında istatistiksel olarak anlamlı farklılık gözlenmedi.

Sonuç: Kemik defekti oluşturulan ratlarda Chitosan Acetate Bandage’nin kemik iyileşmesi üzerine histopatolojik değerlendirmeler göz önüne alındığında olumlu olabileceği sonucuna varılmıştır. 

Keywords

Chitosan Acetate, Kemik iyileşmesi, Travma

Effects of Chitosan Acetate on Early Bone Healing in The Rats That Create Bone Defect

Abstract

Aim: Bone healing is a complex process which
includes pathophisiologic events that need a multidisciplinary aproach. Because
of that most authors have studies on this process. The agents which effect the
bone healing positively, have a clinic usage. The best material to use for bone
healing have to be effected on bone repair, and easy to use. In our study, we
aimed to investigate the effects of Chitosan Acetate Bandage on bone healing.

Materials and Methods:Two separate study groups
were formed each one including ten rats that crate defects on their tibias. In
the first group, the defect had been irrigated by serum phisiologic and the
bone biopsies had taken form bone on the seventh day. In the second group,
Chitosan Acetate Bandage had applied to the defect and the bone biopsies had
taken form bone on the seventh day. The samples evaluated histopathologically
for osteoblastic activite, inflamatory response and fibrosis scores. Ki kare
test was employed to statistically analysis of the data and the significance
line accepted as p<0.05.

Results:Osteoblastic activite (Stage 4) and
inflamatory response (Stage 4) were statistically significant high in Chitosan
group according to normal salin group. However there were no statistically significant
results for fibrosis scores (Stage 3, 4) between two groups.

Conclusion:We determined positive effects of Chitosan
Acetate on rats, that have bone defects by histopathologic evaluations.

Keywords

Chitosan Acetate, Bone healing, Trauma

References

• 1. Keskinoğlu P, İnan F. Analysis of Trauma Cases Admitted To A State Hospital Emergency Department. Gazi Medical Journal 2014; 25: 1-4.
• 2. Pekdemir M, Cete Y, Eray O, Atilla R, Cevik A.A, Topuzoglu A. Determination of the epidemiological characteristics of the trauma patients. Ulusal Travma Dergisi 2000;64:250-4.
• 3. Oryan A, Alidadi S, Moshiri A, Maffulli N. Bone regenerative medicine: classic options, novel strategies, and future directions. J Orthop Surg Res 2014; 9(1):18.
• 4. Toğral G, Arıkan M, Korkusuz P, Hesar R.H, Ekşioğlu M.F. Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur. Joint Diseases and Related Surgery 2015;26(3):137-44.
• 5. Histing T, Marciniak K, Scheuer C, Garcia P, Holstein J.H, Klein M, Matthys R, Pohlemann T, Menger M.D. Sildenafil accelerates fracture healing in mice. J Orthop Res 2011;29:867-73.
• 6. Kumar M.N.V.R. A review of chitin and chitosan applications. React Funct Polym 2000;46:1-27.
• 7. Mucha M, Michalak I, Balcerzak J, Tylman M. Chitosan scaffolds, films and microgranules for medical application-preparation and drug release studies. Polimery 2012;57(10):714-21.
• 8. No H.K, Park N.Y, Lee S.H, Meyers P.S. Antibacterial activity of chitosans and chitosan oligomers with different molecular weights. Int J Food Microbiol 2002;74(1-2):65-72.
• 9. Gerasimenko D.V, Avdienko I.D, Bannikova G.E, Zueva O.Y, Varlamov V.P. Antibacterial effects of water-soluble low-molecular-weight chitosans on different microorganisms. Appl Biochem Microb 2004;40:253-7.
• 10. Dai T, Tegos G.P, Burkatovskaya M, Castano A.P, Hamblin M.R. Chitosan acetate bandage as a topical antimicrobial dressing for infected burns. Antimicrob Agents Chemother 2009;53(2):393-400.
• 11.Ardakani F.E, Navabazam A, Fatehi F, Ardakani M.D, Khadem S, Rouhi G. Histologic evaluation of chitosan as an accelerator of bone regeneration in microdrilled rat tibias. Dent Res J 2012;9(6):694-9.
• 12. Spin-Neto R, de Freitas R.M, Pavone C, Cardoso M.B, Campana-Filho S.P, Marcantonio R.A, Marcantonio E.Histological evaluation of chitosan based biomaterials used for the correction of critical size defects in rat’s calvaria. J Biomed Mater Res A 2010;93:107 4.
• 13. Bostan K, Aldemir T, Aydın A. Kitosan ve antimikrobiyal aktivitesi. Türk Mikrobiyol Cem Derg 2007;37(2):118-27.
• 14. Ross MH, Wojciech P. Bone. In: Histology a test and atlas, with correlated cell and molecular biology. 6th ed. Philadelphia: Lippincott Williams & Wilkins; 2011:218-52.
• 15.Marsell R, Einhorn T.A. The biology of fracture healing. Injury 2011;42(6):551-5.
• 16.Mountziaris P.M, Mikos A.G. Modulation of the inflammatory response for enhanced bone tissue regeneration. Tissue Engineering Part B-Reviews 2008; 14(2):179–86.
• 17. Gerstenfeld L.C, Cullinane D.M, Barnes G.L, Graves D.T, Einhorn T.A. Fracture healing as a post-natal developmental process: molecular, spatial, and temporal aspects of its regulation. Journal of Cellular Biochemistry 2003; 88(5):873–84.
• 18. Sevimli R, Üzel M. Rat tibia diyafiz kırıklarında deksketoprofen trometamolün kırık iyileşmesi üzerine etkisi. M.Acta Orthop Traumatol Turc 2013;47(6):423-9.
• 19. Shin S.Y, Park H.N, Kim K.H, Lee M.H, Choi Y.S, Park Y.J, Lee Y.M, Ku Y, Rhyu I.C, Han S.B, Lee S.J, Chung CP. Biological evaluation of chitosan nanofiber membrane for guided bone regeneration. J Periodontol 2005;76:1778 84.
• 20. Li H, Ge Y, Zhang P, Wu L, Chen S. The effect of layer by layer chitosan hyaluronic acid coating on graft to bone healing of a poly (ethylene terephthalate) artificial ligament. J Biomater Sci Polym Ed 2012;23:425-38.
• 21.Usami Y, Okamoto Y, Takayama T, Shigemasa Y, Minami, S. Chitin and chitosan stimulate canine polymorphonuclear cells to release leukotriene B4 and prostaglandin E2. J Biomed Mat Res 1998;42:517-22.