The effect of negative effects of extremely low-frequency electromagnetic fields on salivary glands

Year 2018, Volume: 15 Issue: 2, 45 - 54, 14.08.2018

Mehmet Sinan Doğan , Abdülsamet Tanik , Mehmet Cihan Yavaş

Abstract

Background: In this study, using histopathological and immunohistochemical methods, we aimed to investigate whether Low-Frequency EMA has any harmful effects on salivary glands and whether Melatonin (MLT) and Ganoderma lucidum (GL) have protective effects against these harmful effects

Material and methods: A total of 56 Wistar albino rats were used in this study. The rats were divided into 7 equal groups and exposed to the EMA produced from high voltage 8 hours of each day. EMA + MLT (10 mg / kg / day) for groups 3 and 6, just EMA for groups 1 and 4, EMA + GL (20 mg / kg / day) for groups 2 and 5 were used. Group 7 was determined as a control group. Rats were administered GL via oral gavage and MLT via the intraperitoneal route. Half of the groups were sacrificed on day 26 and the rest of the groups were sacrificed on day 52. Histopathological and immunohistochemical analyses of the obtained specimens were performed.

Results: As a result of the histopathological analysis, some parameters such asintra and interlobular duct degeneration, inflammatory cell infiltration and blood vessel dilatation and haemorrhage were found statistically significant difference in the groups treated with EMA, (EMA+MLT) and (EMA + GL) compared to the control group on the 26th and 52nd at day (p<0.05). In immunohistochemical analyses, the positive expression of vascular endothelial growth factor (VEGF) and E-cadherin adhesion receptor (E-cadherin) was observed in all groups except for control group.

Conclusion: Exposure to EMA caused histopathological changes in the salivary glands of the rats. The use of MLT and GL in treatment may be protective against these harmful effects.

Keywords

Electromagnetic Field, Melatonin, Ganoderma lucidum, Salivary glands

Düşük frekanslı elektromanyetik alanın tükürük bezleri üzerindeki zararlı etkileri

Abstract

Amaç:Bu çalışmada düşük frekanslı EMA’nin tükürük
bezleri üzerinde herhangi bir zararlı etkisini ve Melatonin (MLT) ve Ganoderma
lucidum(GL)’un bu zararlı etkilere karşı koruyucu bir etkisinin olup olmadığını
histopatolojik ve immunohistokimyasal olarak incelenmesi amaçlanmıştır.

Materyal ve metod:Bu çalışmada düşük frekanslı EMA’nin tükürük
bezleri üzerinde herhangi bir zararlı etkisini ve Melatonin (MLT) ve Ganoderma
lucidum(GL)’un bu zararlı etkilere karşı koruyucu bir etkisinin olup olmadığını
histopatolojik ve immunohistokimyasal olarak incelenmesi amaçlanmıştır.

Bulgular:Yapılan histopatolojik
analiz sonucunda; 26. ve 52. günde ratlar gruplar arası karşılaştırılmasında EMA,
(EMA+MLT) ve (EMA + GL) uygulanan gruplarda intra ve interlobuler kanallarda
dejenerasyon, iltihabi hücre infiltrasyonu ve kan damarlarında dilatasyon ve
hemorajikontrol grubuna göre istatistiksel olarak anlamlı derecede fark
bulunmuştur (p˂0.05). immünohistokimyasal analizlerde ise, Vasküler Endotelyal
Büyüme Faktörü (VEGF) ve E-kadherin yapışma reseptörü(E-kadherin) ekspresyonu kontrol
grubu hariç diğer gruplarda pozitif ekspresyonu gözlemlenmiştir.

Sonuç:EMA'ye maruziyetin ratların tükürük bezlerinde
histopatolojik değişikliklere neden olduğu gözlemlenmiştir. Yapılan tedavilerde
MLT ve GL'nin kullanımı bu zararlı etkilere karşı koruyucu bir etkiye sahip
olabilir.

Keywords

Melatonin, Ganoderma lucidum, Elektromagnetik Alan, Tükürük bezleri

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