Brusellozda Brucellacapt (Immunocapture-agglutination) ve Rose Bengal testlerinin değerlendirilmesi

Year 2015, Volume: 12 Issue: 2, 179 - 184, 30.08.2015

Erkan Yula , Turkan Toka Özer Vicdan Köksaldı Motor Erhan Yengil Melek İnci Hüseyin Aksoy Çetin Kılınç

Abstract

Amaç: Bruselloz, Gram negatif bir bakteri olan brusella türlerinin neden olduğu son derece bulaşıcı bir
zoonotik hastalıktır. İnsan brusellozunun tanısında çok çeşitli serolojik testler kullanılmaktadır. Brusellozun
serolojik tanısında sıklıkla tercih edilen yöntem olan tüp aglütinasyon testi, blokan antikor varlığını tespit
edemediğinden yalancı negatif sonuçlara yol açabilmektedir. Bu çalışmada farklı iki test olan Rose Bengal
testi (RBT) ve immuncapture- aglütinasyon (Brucellacapt) testi sonuçlarının analiz edilmesi ve bu testlerin
kullanılabilirliğinin değerlendirilmesi amaçlanmıştır.
Materyal Metod: Çalışmaya, Ağustos 2009 - Ağustos 2011 tarihleri arasında Kızıltepe Devlet Hastanesi
mikrobiyoloji laboratuarına çeşitli polikliniklerden gelen 220 hastadan alınan serum örneği dahil edilmiştir.
Her bir hasta serumunda hem RBT (ADR Diagnostics, Medico Chemistry) hem de Brucellacapt (ADR
Diagnostics, Medico Chemistry) testleri çalışılmıştır. Her iki test de üretici firmanın talimatları
doğrultusunda gerçekleştirilmiştir. Brucellacapt testinde 1/160 serum titresi eşik değer olarak kabul
edilmiştir. Çalışma grubuna klinik bulgulara dayanarak bruselloz şüphesi olan 220 hasta dahil edilmiştir.
Bulgular: Serum örneklerin serolojik olarak incelenmesi ile her iki test 146 (%66,4) hastada negatif
bulunurken, hem RBT hem de Brucellacapt testinin pozitif olduğu hasta sayısı ise 59 (%26,8) olarak
bulunmuştur. Ek olarak sadece RBT testinin pozitif olduğu hasta sayısı 9 (%4,1), sadece Brucellacapt
testinin pozitif olduğu hasta sayısı ise 6 (%2,7) olarak tespit edilmiştir. Toplamda RBT 68 hastada (%30,9),
Brucellacapt testi ise 65 hastada (%29,5) pozitif olarak bulunmuştur. Sonuç: Bölgemizde brusellozun endemik bir problem olarak devam ettiği tespit edilmiştir.
RBT, bruselloz tanısında, tecrübeli eleman gerektirmeyen, yorumlanması kolay, maliyeti düşük ve
teknik olarak da kolay uygulanabilir bir testdir. Brucellacapt testi de kolay ve pratik bir test olup bruselloz
tanısında tamamlayıcı bir test olarak kullanılabilir

Keywords

Bruselloz, Rose Bengal, Aglütinasyon

Assesment of Brucellacapt (Immunocapture-agglutination) and Rose Bengal tests in human brucellosis

Abstract

OBJECTIVE: Brucellosis is extremely infectious zoonosis caused by the Gram-negative bacteria of the
genus Brucella. Several serological tests have been used for the diagnosis of human brucellosis. Although
standard tube agglutination test is the frequently preferred method for the serodiagnosis of brucellosis, it may
cause false negative results due to its incompetence in detecting the blocking antibodies. In this study,
analysis of the results obtained with two different tests which are Rose Bengal test (RBT) and
immunocapture- agglutination test (Brucellacapt) for detection of Brucella-specific antibodies in an area of
endemicity and evaluation of usefulness of these tests were aimed.
METHODS: Atotal of 220 sera from different groups of patients from various polyclinics were included the
study between August 2009 and August 2011 dates. Rose Bengal test (ADR Diagnostics, MedicoChemistry)
and Brucellacapt (ADR Diagnostics, Medico- Chemistry) were performed for each serum
sample. Both of the tests were performed according to manufacturer's recommendations. The threshold
serum titre was accepted as 1/160 in Brucellacapt test. In this study, 220 patients with suspected brucellosis
based on clinical findings were included.
RESULTS: As a result of Serological examination of the clinical samples both of the RBT
and Brucellacapt tests were negative in 146 (66.4%) patients. Both of the RBT and Brucellacapt tests were
positive in 59 (26.8%) patients. In addition, only RBTwas positive in 9 (4.1%) patients and only Brucellacapt
test was positive in 6 (2.7%) patients. Totally, RBT was positive in 68 patients (30.9%) and Brucellacapt test
was positive in 65 patients (29.5%).
CONCLUSION: Brucellosis continues to be an endemic problem in our region. RBTis a very useful test for
the diagnosis of human brucellosis and hasn't got technical difficulties since they didn't require skilled
personnel and high-cost material and easy to interpret. Brucellacapt is also simple and practical test and it is
suitable complementary test for diagnosis of brucellosis.

Keywords

Brucellosis, Rose Bengal, Agglutination

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