Investigation of The Ameliorative Effects of Propolis Against Skin Damage in Experimental Diabetes.

Year 2021, Volume: 18 Issue: 2, 322 - 328, 27.08.2021

Sibel Türedi

https://doi.org/10.35440/hutfd.982441

Abstract

Background: The purpose of this study was to investigate the possible positive effects of propolis against the damage caused by experimental Diabetes Mellitus on the dermal tissue with histopathological parameters.
Methods: Twenty-four male Wistar Albino rats aged 10-12 weeks were randomly assigned into four groups as; Control, Propolis 100mg, DM and DM+Propolis (100mg). Experimental diabetes was induced with a single dose of 60 mg/kg streptozotocin (intraperitoneally) dissolved in 0.1 Molar (Ph: 4.5) citrate buffer. At the end of the experiment period (Day 28), all groups were sacrificed and histopathological evaluation was performed on the dermal tissues by Hematoxylin&Eosin and Masson Trichrome staining.
Results: In histopathological evaluation; normal morphological structure were observed in the skin samples of the control and Propolis 100mg groups. Thinning of the multilayered epithelium, degeneration, separation and decrease in the connective tissue in the dermis layer was observed in DM group, but these findings were significantly improved in the DM+Propolis 100 mg group.
Conclusions: The present study showed that the biological properties of Propolis 100mg dose may prevent skin damage caused by diabetes at the morphological level in dermal tissue.
Key words: Skin, Diabetes Mellitus, Propolis, Histopathology, Rat

Keywords

Skin, Diabetes Mellitus, Propolis, Histopathology, Rat

Deneysel Diyabette Deri Hasarına Karşı Propolisin İyileştirici Etkilerinin Araştırılması

Abstract

Özet
Amaç: Bu çalışmada deneysel Diabetes Mellitus’un dermal doku üzerinde oluşturduğu hasara karşı propolisin muhtemel olumlu etkilerini histopatolojik parametreler ile araştırmaktır.
Materyal ve Metod: Çalışmamızda 24 adet 10-12 haftalık Wistar Albino türü erkek sıçanlar Kontrol, Propolis 100mg, DM ve DM+Propolis (100mg) olarak rastgele 4 gruba ayrıldı. 0.1 Molar (Ph: 4,5) sitrat tamponunda çözülen tek doz 60 mg/kg Streptozotosin (inroperitoneal) ile deneysel diyabet oluşturuldu. Deney süresinin bitiminde (28. Gün) tüm gruplar sakrifiye edildi ve elde edilen dermal dokularda Hematoksilen&Eozin ve Masson Trikrom boyamaları ile histopatolojik değerlendirme yapıldı.
Bulgular: Yapılan histopatolojik değerlendirmede; kontrol ve Propolis 100mg gruplarına ait deri örneklerinin normal morfolojik yapıda olduğu izlendi. DM grubunda çok tabakalı epitelde incelme, dejenerasyon, dermis tabakasında yer alan bağ dokusunda ayrılma ve azalma gözlenirken DM+Propolis 100 mg grubunda ise bu bulgularda belirgin şekilde bir iyileşme izlendi.
Sonuç: Bu çalışma, Propolis 100mg dozunun biyolojik özelliklerin diyabetin dermal dokuda meydana getirdiği cilt hasarını morfolojik düzeyde önleyebileceğini göstermiştir.
Anahtar kelimeler: Deri, Diabetes Mellitus, Propolis, Histopatoloji, Rat

Keywords

Deri, Diabetes Mellitus, Propolis, Histopatoloji, Rat

References

• 1- Su HC, Hung LM, Chen JK. Resveratrol, a red wine antioxidant, possesses an insulin-like effect in streptozotocin-induced diabetic rats. Am J Physiol Endocrinol Metab. 2006; 290(6):1339-46.
• 2- Tunali S, Yanardag R. Protective effect of vanadyl sulfate on skin injury in streptozotocin-induced diabetic rats. Hum Exp Toxicol. 2013, 32(11):1206-12.
• 3- Suh Y, Moon J, Yoon JY, Kim SW, Choi YS. Effects of Initiation Time of Glycemic Control on Skin Collagen Recovery in Streptozotocin-Induced Diabetic Rats. Dermatology. 2018, 234(3-4):148-56.
• 4- Tosi B, Donini A, Rmagnoli C, Bruni A. Antimicrobial Activity and Chemical Extracts of Propolis Prepared with Different Solvents. Phytotherapy Research. 1996, 10(4):335-36.
• 5- Scheller S, Wilczok T, Imielski S, Krol W, Gabrys J, Shani J. Free radical scavenging by ethanol extract of propolis. Int J Radiat Biol. 1990; 57(3): 461-65.
• 6- Rezzani R, Giugno L, Buffoli B, Bonomini F, Bianchi R. The protective effect of caffeic acid phenethyl ester against cyclosporine A induced cardiotoxicity in rats. Toxicology. 2005; 212(2-3):155-64.
• 7- Mollaoglu H, Gokcimen A, Ozguner F, Oktem F, Koyu A, Kocak A, Demirin H, Gokalp O, Cicek E. Caffeic acid phenethyl ester prevents cadmium-induced cardiac impairment in rat. Toxicology. 2006;227(1-2):15-20.
• 8- Okutan H, Ozcelik N, Yilmaz HR, Uz E. Effects of caffeic acid phenethyl ester on lipid peroxidation and antioxidant enzymes in diabetic rat heart. Clin Biochem. 2005;38(2):191-6.
• 9- Tohamy AA, Abdella EM, Ahmed RR, Ahmed YK. Assessment of anti-mutagenic, anti-histopathologic and antioxidant capacities of Egyptian bee pollen andpropolis extracts. Cytotechnology. 2014;66(2):283-97.
• 10- Akyol S, Ugurcu V, Altuntas A, Hasgul R, Cakmak O, Akyol O. Caffeic acid phenethyl ester as a protective agent against nephrotoxicity and/or oxidative kidney damage: adetailed systematic review. ScientificWorldJournal. 2014;2014 Jun 3. doi: 10.1155/2014/561971. [Epub ahead of print].
• 11- Murata K, Yatsunami K, Fukuda E, Onodera S, Mizukami O, Hoshino G, Kamei T. Antihyperglycemic effects of propolis mixed with mulberry leaf extract on patients with type 2 diabetes. Altern Ther Health Med. 2004;10(3):78-9.
• 12- Askari VR, Rahimi VB, Zamani P, Fereydouni N, Rahmanian-Devin P, Sahebkar AH, Rakhshandeh H. Evaluation of the effects of Iranian propolis on the severity of post operational-induced peritoneal adhesion in rats. Biomed Pharmacother. 2018;99:346-353.
• 13- Ates O, Cayli SR, Yucel N, Altinoz E, Kocak A, Durak MA, Turkoz Y, Yologlu S. Central nervous system protection by resveratrol in streptozotocin-induced diabetic rats. J Clin Neurosci. 2007;14(3):256-60.
• 14- Demirseren DD, Emre S, Akoglu G, Arpaci D, Arman A, Metin A, Çakır B. Relationship between skin diseases and extracutaneous complications of diabetes mellitus: clinical analysis of 750 patients. Am J Clin Dermatol. 2014;15: 65–70.
• 15- Chen X, Lin W, Lu S, Xie T, Ge K, Shi Y, Zou J, Liu Z, Liao W. Mechanistic study of endogenous skin lesions in diabetic rats. Exp Dermatol. 2010;19(12):1088-95.
• 16- Greig A V, James S E, McGrouther D A, Terenghi G, Burnstock G. Purinergic receptor expression in the regeneration epidermis in a rat model of normal and delayed wound healing. Exp Dermatol. 2003;12: 860–71.
• 17- Wang C M, Lincoln J, Cook J E, Becker D L. Abnormal connexin expression underlies delayed wound healing in diabetic skin. Diabetes 2007;56: 2809– 817.
• 18- Rajkumar L, Srinivasan N, Balasubramanian K, Govindarajulu P: Increased degradation of dermal collagen in diabetic rats. Indian J Exp Biol. 1991;29: 1081–083.
• 19- Argyropoulos AJ, Robichaud P, Balimunkwe RM, Fisher GJ, Hammerberg C,Yan Yan Y, Quan T. Alterations of Dermal Connective Tissue Collagen in Diabetes: Molecular Basis of Aged-Appearing Skin. PLoS One.2016 Apr 22;11(4):e0153806. doi: 10.1371/journal.pone.0153806.
• 20- Wess TJ. Collagen fibril form and function. Adv Protein Chem. 2005;70: 341–74.
• 21- Reiser KM. Nonenzymatic glycation of collagen in aging and diabetes. Proc Soc Exp Biol Med. 1998 218: 23–37.
• 22- Okano Y, Masaki H, Sakurai H: Dysfunction of dermal fibroblasts induced by advanced glycation end-products (AGEs) and the contribution of a nonspecific interaction with cell membrane and AGEs. J Dermatol Sci. 2002;29:171–80.
• 23- Nna VU, Bakar ABA, Ahmad A,Eleazu CO, Mohamed M. Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin-Induced Diabetic Rats: Combined Protective Effects of Malaysian Propolis and Metformin. Antioxidants (Basel). 2019; 8(10):465.
• 24- Burdock GA. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem. Toxicol. 1998;36(4):347–63.
• 25- Çilenk KT, Öztürk İ, Sönmez MF. 2016. Ameliorative effect of propolis on the cadmium-induced reproductive toxicity in male albino rats. Exp Mol Pathol. 101:207–213.
• 26- Yuluğ E, Türedi S, Yıldırım Ö, Yenilmez E, Aliyazıcıoğlu Y, Demir S, Özer-Yaman S, Menteşe A. Biochemical and morphological evaluation of the effects of propolis on cisplatin induced kidney damage in rats. Biotech Histochem. 2019 ;94(3):204-13.
• 27- Usman UZ, Bakar ABAB, Mohamed M. Propolis improves pregnancy outcomes and placental oxidative stress status in streptozotocin-induced diabetic rats BMC Complement Altern Med. 2018 Dec 5;18(1):324. doi: 10.1186/s12906-018-2391-6.
• 28- Bazmandegan G, Boroushaki MT, Shamsizadeh A, Ayoobi F, Hakimizadeh E, Allahtavakoli M. Brown Oxidative Medicine and Cellular Longevity propolis attenuates cerebral ischemia-induced oxidative damage via affecting antioxidant enzyme system in mice. Biomedicine &Pharmacotherapy. 2017 Jan;85:503-510. doi: 10.1016/j.biopha.2016.11.057.
• 29- Afsharpour F, Javadi M, Hashemipour S, Koushan Y, Haghighian HK. Propolis supplementation improves glycemic and antioxidant status in patients with type 2 diabetes: A randomized, double-blind, placebo-controlled study. Complement Ther Med. 2019 Apr;43:283-288. doi: 10.1016/j.ctim.2019.03.001.