Synergistic Effect of Aerobic Exercise Intensity and Creatine Monohydrate Supplementation on Energy Metabolism Biomarkers in Mice Cardiac Ventricular Muscle Tissue

Hakim Çelik; Abdullah Taşkın

doi:10.35440/hutfd.1573754

Research Article

Aerobik Egzersiz Yoğunluğunun ve Kreatin Monohidrat Takviyesinin Fare Kardiyak Ventrikül Kas Dokusunda Enerji Metabolizması Biyobelirteçleri Üzerindeki Sinerjitik Etkisi

Year 2024, Volume: 21 Issue: 3, 469 - 475

Hakim Çelik , Abdullah Taşkın

https://doi.org/10.35440/hutfd.1573754

Abstract

Amaç: Kreatin monohidrat (CrM) takviyesinin ve farklı yoğunluktaki aerobik egzersizin farelerin kardiyak ventriküler kas dokusundaki enerji metabolizmasının temel biyobelirteçleri üzerindeki kombine etkile-rini araştırmaktır.
Materyal ve Metod: Kırk iki erkek BALB/c faresi rastgele altı gruba ayrıldı (grup başına n=7): kontrol (C), CrM takviyesi (C+CrM), düşük yoğunluklu egzersiz (LIE), CrM takviyesiyle LIE (LIE+CrM), yüksek yoğunluklu egzersiz (HIE) ve CrM takviyesiyle HIE (HIE+CrM). Sekiz hafta boyunca, egzersiz grupları haftada beş gün koşu bandında egzersiz uygulanırken, CrM grupları %4 CrM ile zenginleştirilmiş bir diyet aldı. Anahtar biyobelirteçler; miyosit güçlendirici faktör 2A (MEF2A), monokarboksilat taşıyıcı 1 (MCT1), pirüvat dehi-drogenaz (PDH), peroksisom proliferatör aktive reseptör gama koaktivatör 1-alfa (PGC-1α) ve mitokondri-yal transkripsiyon faktörü A (TFAM) ELISA testleri kullanılarak kardiyak ventriküler kas dokusunda kantifize edildi.
Bulgular: LIE+CrM grubu, C+CrM grubuna kıyasla MEF2A'da önemli bir artış gösterdi (p<0,05). PDH seviye-leri, hem LIE+CrM hem de HIE+CrM gruplarında, C ve C+CrM gruplarına kıyasla önemli ölçüde daha yüksekti (p<0,05). PGC-1α seviyeleri, LIE+CrM grubunda en yüksekti ve istatistiksel anlamlılığa yaklaşıyor-du (p=0,051). TFAM ekspresyonu, LIE+CrM grubunda, LIE, HIE ve C+CrM gruplarına kıyasla önemli ölçüde yüksekti (p<0,05). MCT1 seviyeleri, LIE+CrM grubunda anlamlı olmayan bir artış eğilimi gösterdi.
Sonuç: Düşük yoğunluklu aerobik egzersizle birleştirilen CrM takviyesi, kardiyak ventriküler kas dokusun-da mitokondriyal biyogenez ve enerji metabolizmasıyla ilişkili temel biyobelirteçleri önemli ölçüde artırır. Bu bulgular, kardiyak enerji metabolizmasını optimize edebilecek ve kardiyovasküler sağlığı iyileş-tirebilecek sinerjik bir etkiye işaret ediyor.

Keywords

Kreatin monohidrat, Aerobik egzersiz yoğunluğu, Kardiyak enerji metabolizması, Mitokondriyal biyogenez

Project Number

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