Deneysel Akut Pankreatit Oluşturulan Ratlarda Lipit Profil Üzerine Timokinonun Etkileri

Year 2022, , 58 - 65, 15.05.2022

Sevim Kanaç Deniz Uluışık , Ercan Keskin

https://doi.org/10.31196/huvfd.1066860

Abstract

Çalışmada serulein ile akut pankreatit oluşturulan ratlarda timokinonun lipit profil üzerine olası etkilerinin belirlenmesi amaçlanmıştır. Çalışma süresi olan 9 gün boyunca kontrol grubuna (K) herhangi bir uygulama yapılmadı. Timokinon grubundaki (TQ) hayvanlara 9 gün süre ile günde 20 mg/kg timokinon intraperitoneal olarak verilirken, akut pankreatit grubundaki (AP) hayvanlarda araştırmanın 7. gününde 50 μg/kg ve 2 saat sonra 25 μg/kg seruleinin intraperitoneal olarak uygulanmasıyla akut pankreatit oluşturuldu. Akut pankreatit+timokinon grubunda ise (AP+TQ) günde 20 mg/kg olmak üzere 9 gün süre ile intraperitoneal olarak timokinon verilen hayvanlarda araştırmanın 7. gününde timokinon uygulamasından 2 saat sonra 50 μg/kg ve bundan 2 saat sonra 25 μg/kg seruleinin intraperitoneal olarak uygulanmasıyla akut pankreatit oluşturuldu. Bütün hayvanlardan alınan kan örneklerinde Lipaz, Amilaz, Trigliserit, Total Kolesterol, Düşük dansiteli lipoprotein (LDL-kolesterol) ve Yüksek dansiteli lipoprotein (HDL-kolesterol) düzeyleri belirlendi. Deneysel akut pankreatit oluşturulan ratlarda amilaz ve lipaz düzeyleri kontrol grubuna göre anlamlı olarak yüksek olduğu belirlendi (p<0.05). Akut pankreatit oluşturulan grupta yüksek olan bu enzim düzeylerinin timokinon uygulanan akut pankreatitli ratlarda önemli oranda baskılandığı belirlendi (p<0.05). Çalışmada serulein ile oluşturulan deneysel akut pankreatite bağlı olarak plazma trigliserit ve total kolesterol düzeylerinin kontrol grubuna göre önemli oranda yüksek olduğu belirlendi (p<0.05). Akut pankreatitli ratlarda LDL-kolesterol düzeyi kontrol grubuna göre anlamlı oranda yüksek bulunurken (p<0.05), HDL-kolesterol düzeyi ise anlamlı oranda düşüktü (p<0.05). Timokinon uygulanan akut pankreatitli ratların total kolesterol ve LDL-kolesterol düzeylerinin akut pankreatitli gruba göre anlamlı oranda düşük olduğu belirlendi (p<0.05). Sonuç olarak, plazma lipit parametreleri ve bazı enzim düzeylerindeki farklılıklar dikkate alındığında timokinonun akut pankreatit üzerine olumlu etkileri olduğu kanaatine varıldı.

Keywords

Serulein, Timokinon, Lipit Profil, Rat

Supporting Institution

Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

19202065

Thanks

Bu araştırma Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından 19202065 proje numarası ile desteklenmiştir.

Effects of Thymoquinone on Lipid Profile in Rats with Experimentally Acute Pancreatitis

Abstract

This study aimed to determine the possible effects of thymoquinone on lipid profile in rats with cerulein-induced acute pancreatitis. No application was made to the control group (K) during the study period of nine days. The thymoquinone group (TQ) animals were administered 20 mg/kg of thymoquinone daily for nine days. In the acute pancreatitis group (AP), acute pancreatitis was induced by intraperitoneal cerulein administration of 50 μg/kg and 2 hours later, 25 μg/kg on the seventh day of the study. Animals in the acute pancreatitis+thymoquinone group (AP+TQ) were intraperitoneally administered 20 mg/kg of thymoquinone daily for nine days. On the seventh day of the study, acute pancreatitis was induced by intraperitoneal administration of 50 μg/kg and 2 hours later 25 μg/kg of cerulein after 2 hours from thymoquinone administration. In blood samples taken from all animals, Lipase, Amylase, Triglyceride, Total Cholesterol, low-density lipoprotein (LDL-cholesterol), and high-density lipoprotein (HDL-cholesterol) levels were determined. The amylase and lipase levels of rats with acute experimental pancreatitis were significantly higher than the control group (p<0.05). It was determined that these enzyme levels, which were high in the acute pancreatitis group, were significantly suppressed in rats with acute pancreatitis treated with thymoquinone (p<0.05). The increase in plasma triglyceride and total cholesterol levels due to acute experimental pancreatitis induced with cerulein was significant compared to the control group (p<0.05). LDL-cholesterol level of rats with acute pancreatitis was found to be significantly higher than the control group (p<0.05), while HDL-cholesterol level was significantly lower (p<0.05). The total cholesterol and LDL-cholesterol levels of rats with acute pancreatitis treated with thymoquinone were significantly lower than those of the acute pancreatitis group (p<0.05). In conclusion, in light of differences in plasma lipid parameters and some enzyme levels, it was considered that thymoquinone has positive effects on acute pancreatitis.

Keywords

Cerulein, Thymoquinone, Lipid Profile, Rats

Project Number

19202065

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