Karaciğer Kelebekleriyle Doğal Enfekte Küçük Ruminant Karaciğerlerinde Sindekan-1 Salınımının İmmunohistokimyasal Olarak Değerlendirilmesi

Abstract

Bu çalışmada karaciğer kelebekleri ile enfekte olan ruminnatlara ait karaciğer dokularında meydana gelen hasarın patolomorfolojisi ve bu hasarda sindekan-1 proteinin rolü araştırıldı. Çalışmada karaciğer paraziti tespit edilen 62 ruminnata ait karaciğer doku örneği kullanıldı. Elde edilen dokularda histopatolojik inceleme amacıyla hematoksilen eosin boyaması yapıldı. Sindekan-1 proteinin belilenmesi amacıyla streptavidin-biotin-peroksidaz (ABC) yöntemiyle immunohistokimyasal boyamalar yapıldı. Makroskobik incelmede enfekte karaciğerlerde konjesyon, kanama, nekroz ve fibrozis görüldü. Mikroskobik incelmede ise şiddetli karaciğer kesitlerinde kanama, inflamasyon, dejenerasyon, nekroz ve safra kanallarında hiperplazi gözlendi. İmmunohistokimyasal olarak enfekte karaciğer dokularında sindekan-1 proteinin şiddetli immunopozitif reaksiyon verdiği tespit edildi. Bu çalışma sonucunda karaciğer kelebeklerinin neden olduğu karaciğer hasarında sindekan-1 proteinin salınımının arttığı belirlenmiştir.

Keywords

• Sindekan-1
• karaciğer
• küçük ruminant
• immunohistokimya

Immunohistochemical Evaluation of Syndecan-1 Expression in the Liver of Small Ruminants with Natural Liver Fluke Infection

Abstract

This study was conducted to investigate the pathomorphology of damage in liver tissues of ruminants infected with liver flukes and the role of syndecan-1 protein in this damage. In the study, liver tissue samples from 62 ruminants with liver parasites were used. Histopathological examination of these tissues was performed using hematoxylin-eosin staining. An immunohistochemical staining procedure was performed through the streptavidin-biotin-peroxidase (ABC) method to determine the syndecan-1 protein. Upon the macroscopic examination, congestion, hemorrhage, necrosis, and fibrosis were observed in infected livers. Hemorrhage, inflammation, degeneration, necrosis, and hyperplasia of the bile ducts were observed in severe liver sections upon microscopic examination. Syndecan-1 protein immunohistochemically exhibited a strong immunopositive reaction in infected liver tissues. This study concluded that the release of syndecan-1 protein increased liver damage induced by liver flukes.

Keywords

• Syndecan-1
• liver
• small ruminant
• immunohistochemistry

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