Immunohistochemical Evaluation of Syndecan-1 Expression in the Liver of Small Ruminants with Natural Liver Fluke Infection

Year 2022, , 73 - 77, 15.05.2022

Nihat Yumuşak , Kürşat Filikci

https://doi.org/10.31196/huvfd.1076744

Cited By: 1

Abstract

This study was conducted to investigate the pathomorphology of damage in liver tissues of ruminants infected with liver flukes and the role of syndecan-1 protein in this damage. In the study, liver tissue samples from 62 ruminants with liver parasites were used. Histopathological examination of these tissues was performed using hematoxylin-eosin staining. An immunohistochemical staining procedure was performed through the streptavidin-biotin-peroxidase (ABC) method to determine the syndecan-1 protein. Upon the macroscopic examination, congestion, hemorrhage, necrosis, and fibrosis were observed in infected livers. Hemorrhage, inflammation, degeneration, necrosis, and hyperplasia of the bile ducts were observed in severe liver sections upon microscopic examination. Syndecan-1 protein immunohistochemically exhibited a strong immunopositive reaction in infected liver tissues. This study concluded that the release of syndecan-1 protein increased liver damage induced by liver flukes.

Keywords

Syndecan-1, liver, small ruminant, immunohistochemistry

Thanks

The author would like to thank Prof.Dr. Mehtap GUL ATLAS for contribute to parasitological procedures during the course of the study.

Karaciğer Kelebekleriyle Doğal Enfekte Küçük Ruminant Karaciğerlerinde Sindekan-1 Salınımının İmmunohistokimyasal Olarak Değerlendirilmesi

Abstract

Bu çalışmada karaciğer kelebekleri ile enfekte olan ruminnatlara ait karaciğer dokularında meydana gelen hasarın patolomorfolojisi ve bu hasarda sindekan-1 proteinin rolü araştırıldı. Çalışmada karaciğer paraziti tespit edilen 62 ruminnata ait karaciğer doku örneği kullanıldı. Elde edilen dokularda histopatolojik inceleme amacıyla hematoksilen eosin boyaması yapıldı. Sindekan-1 proteinin belilenmesi amacıyla streptavidin-biotin-peroksidaz (ABC) yöntemiyle immunohistokimyasal boyamalar yapıldı. Makroskobik incelmede enfekte karaciğerlerde konjesyon, kanama, nekroz ve fibrozis görüldü. Mikroskobik incelmede ise şiddetli karaciğer kesitlerinde kanama, inflamasyon, dejenerasyon, nekroz ve safra kanallarında hiperplazi gözlendi. İmmunohistokimyasal olarak enfekte karaciğer dokularında sindekan-1 proteinin şiddetli immunopozitif reaksiyon verdiği tespit edildi. Bu çalışma sonucunda karaciğer kelebeklerinin neden olduğu karaciğer hasarında sindekan-1 proteinin salınımının arttığı belirlenmiştir.

Keywords

Sindekan-1, karaciğer, küçük ruminant, immunohistokimya

References

• Al-khafaji MA, et al., 2020: A retrospective survey with post mortem examination of liver fukes and lung hydatidosis in livestock in Babil, Iraq. Plant Archives 20 (2), 4537-4543.
• Belina D, Demissie T, Ashenafi H, Tadesse A, 2015: Comparative pathological study of liver fluke infection in ruminants. Indian J Vet Pathol, 39 (2), 113-120.
• Boray JC, 2017: Liver fluke disease in sheep and cattle. Primefact 446. In: Hutchinson, G.W., Love, S. (Eds.), NSW Depart Prim Indust, 1-14.
• Dar JS, Tak IR, Ganai BA, Shahardar RA, Gazanfar K, 2018: Gross pathological and histopathological changes in the liver and bile duct of Sheep with acute and chronic fasciolosis. Int J Adv Res Sci Engg, 7(4), 2031-2044.
• Deger Y, et al., 2008: Lipid peroxidation and antioxidant potential of sheep liver infected naturally with distomatosis. Türkiye Parazitol Derg, 32 (1), 23-26.
• Hayashida K, Chen Y, Bartlett AH, Park PW, 2008: Syndecan-1 is an in vivo suppressor of Gram-positive toxic shock. J Biol Chem, 283, 19895-19903.
• Kaplan RM, 2001: Fasciola hepatica: a review of the economic impact in cattle and considerations for control. Vet Ther, 2, 40-50.
• Khan SA, Muhammad S, Khan MM, Khan MT, 2015: Study on the prevalence and gross pathology of liver fluke infestation in sheep in and around Quetta District Pakistan. Adv Anim Vet Sci, 3(3), 151-155.
• Kitila, DB, Megersa YC, 2014: Pathological and serum biochemical study of liver fluke infection in ruminants slaughtered at ELFORA Export Abattoir, Bishoftu, Ethiopia. Global J Med Res, 14, 6-20.
• Li J, Yuan T, Zhao X, Lv GY, Liu HQ, 2016. Protective effects of sevoflurane in hepatic ischemia-reperfusion injury. Int J Immunopathol Pharmacol, 29 (2), 300-307.
• Mas-Coma S, Valero MA, Bargues MD, 2009: Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Adv Parasitol, 69, 41-146.
• Matsumoto A, Ono M, Fujimoto Y, Gallo RL, Bernfield M, Kohgo Y, 1997: Reduced expression of syndecan-1 in human hepatocellular carcinoma with high metastatic potential. Int J Cancer, 74, 482-491.
• Mendes EA, Vasconcelos AC, Lima WDS, 2012: Histopathology of Fasciola hepatica infection in Merionesunguiculatus. Rev Patol Trop, 41 (1), 55-62.
• Metwaly HA, Al-Gayyar MM, Eletreby S, Ebrahim MA, El-Shishtawy MM, 2012: Relevance of serum levels of interleukin-6 and syndecan-1 in patients with hepatocellular carcinoma. Sci Pharm, 80, 179-188.
• Nam EJ, Hayashida K, Aquino RS, Couchman JR, Kozar RA, Liu J, Park PW, 2017: Syndecan‐1 limits the progression of liver injury and promotes liver repair in acetaminophen‐induced liver injury in mice. Hepatology, 66 (5), 1601-1615.
• Okoye IC, Egbu FMI, Ubachukwu PO, Obiezue NR, 2015: Liver histopathology in bovine Fascioliasis. Afr J Biotechnol, 14 (33), 2576-2582.
• Oyarzún-Ruiz, Pablo, et al., 2014: Histopathological findings of Fasciola hepatica infection in non-native European hare (Lepus europaeus) in Southern Chile. Rev Bras Parasitol Vet, 28, 145-150.
• Rahko T, 1969: The pathology of natural Fasciola hepatica infection in cattle. Pathol Vet, 6(3), 244-256.
• Regos E, Karászi K, Reszegi A, Kiss A, Schaff Z, Baghy K, Kovalszky I, 2020: Syndecan-1 in liver diseases. Pathol Oncol Res, 26 (2), 813-819.
• Roskams T, Rosenbaum J, De Vos RITA, David G, Desmet V, 1996: Heparan sulfate proteoglycan expression in chronic cholestatic human liver diseases. Hepatology, 24 (3), 524-532.
• Schweizer G, Braun U, Deplazes P, Torgerson PR, 2005: Estimating the financial losses due to bovine fasciolosis in Switzerland. Vet Rec, 157, 188-193.
• Slifko TR, Smith HV, Rose JB, 2000: Emerging parasite zoonoses associated with water and food. Int J Parasitol, 30, 1379-93.
• Talukder S, et al., 2010: Pathological investigation of liver fluke infection of slaughtered black Bengal goat in a selected area of Bangladesh. Bangladesh J Vet Med, 8 (1), 35-40.
• Xia J, Jiang S-c, Peng HJ, 2015: Association between Liver Fluke Infection and Hepatobiliary Pathological Changes: A Systematic Review and Meta-Analysis. PLoS ONE, 10 (7), e0132673.
• Zvibel I, Halfon P, Fishman S, Penaranda G, Leshno M, Or AB, Halpern Z, Oren R 2009: Syndecan 1 (CD138) serum levels: a novel biomarker in predicting liver fibrosis stage in patients with hepatitis C. Liver Int, 29, 208-212.