Simental İnek Sütü ve Süt Hücrelerinde Katalaz, İndirgenmiş Glutatyon, Malondialdehit, Vitamin C ve Total Protein Düzeyleri Arasındaki İlişkilerin Araştırılması

Year 2022, , 78 - 83, 15.05.2022

Filiz Kazak , Pinar Peker

https://doi.org/10.31196/huvfd.1084733

Abstract

Bu çalışmada, Simmental inek sütü ve süt hücrelerinin bazı antioksidan parametreleri, katalaz (CAT) aktiviteleri, indirgenmiş glutatyon (GSH), malondialdehit (MDA), vitamin C (Vit C) ve total protein düzeylerinin belirlenmesi ve bu parametreler arasındaki korelasyonların ortaya çıkarılması amaçlanmıştır. Özel bir çiftlikten klinik olarak sağlıklı 28 inekten toplanan süt örnekleri (her biri 15 mL), California Mastitis Testi (CMT) ile test edilmiş ve CMT negatif örnekler çalışmaya dahil edilmiştir. Kısaca, süt hücreleri santrifüjleme ile 15 mL sütten izole edildi ve ardından sonikasyona tabi tutuldu. Süt ve süt hücresi CAT aktiviteleri, GSH, MDA, Vit C ve TP seviyeleri spektrofotometrik yöntemlerle belirlendi. TP seviyeleri 1 mL sütün süt hücresinde 0.043 ± 0.008 mg ve sütte 34.28 ± 0.656 mg/mL belirlendi. GSH seviyeleri süt hücrelerinde 21.19 ± 1.834 nmol/mg protein, sütte 25.78 ± 3.054 nmol/mL ölçüldü. CAT aktiviteleri süt hücrelerinde 0.13 ± 0.017 U/mg protein ve sütte 2.391 ± 0.277 U/mL bulundu. Sütte MDA seviyeleri 2.27 ± 0.180 nmol/mL, Vit C seviyeleri sütte 68.89 ± 4.226 μg/mL belirlendi. Korelasyonlar açısından: Süt hücresi GSH ve süt GSH seviyeleri, süt hücresi TP düzeyleri ile negatif korelasyon gösterdi (p<0.01). Süt hücresi GSH seviyeleri, süt GSH seviyeleri ile pozitif korelasyon gösterdi (p<0.05). Süt Vit C seviyeleri ile süt TP seviyeleri arasında pozitif korelasyon vardı (p<0.01). Zayıf olmasına rağmen süt CAT aktiviteleri ile süt Vit C düzeyleri arasında pozitif korelasyon vardı (p=0.05). Sonuç olarak, sunulan çalışmada Simmental inek sütü ve süt hücrelerinin bazı biyokimyasal parametreleri (CAT, GSH, MDA, VitC ve TP) değerlendirildi ve tartışıldı. Süt hücrelerinin antioksidan kapasitelerinin artırılabilmesi sayesinde meme sağlığının olumlu etkileneceği düşünülmektedir.

Keywords

CAT, MDA, rGSH, Simmental milk cell, Vit C

Investigating Relationships between Catalase, Reduced Glutathione, Malondialdehyde, Vitamin C, and Total Protein Levels in Simmental Cow’s Milk and Milk Cells

Abstract

In this study, to evaluate some antioxidant parameters of Simmental dairy cow’s milk and milk cells, the catalase (CAT), reduced glutathione (GSH), malondialdehyde (MDA), vitamin C (Vit C), and total protein (TP) were determined, and correlations between these parameters were revealed. The milk samples, collected from 28 clinically healthy cows from a private farm, were tested by CMT. Furthermore, CMT negative samples were included in the study. Briefly, milk cells were isolated from 15 mL of milk by centrifugation, and then they were sonicated. Milk and milk cell CAT activities, GSH, MDA, Vit C, and TP levels were determined by spectrophotometric methods. TP levels were 0.043 ± 0.008 mg in milk cell of 1 mL milk and 34.28 ± 0.656 mg/mL in milk. GSH levels were 21.19 ± 1.834 nmol/mg protein in milk cells and 25.78 ± 3.054 nmol/mL in milk. CAT activities were 0.13 ± 0.017 U/mg protein in milk cells and 2.391 ± 0.277 U/mL in milk. MDA levels were 2.27 ± 0.180 nmol/mL and Vit C levels were 68.89 ± 4.226 μg/mL in milk. As regards correlations: Milk cell GSH and milk GSH levels were negatively correlated with milk cell TP levels (p<0.01). Milk cell GSH levels were positively correlated with milk GSH levels (p<0.05). Milk Vit C levels were positively correlated with milk TP levels (p<0.01). Although it was weak, there was a positive correlation between milk CAT activities and milk Vit C levels (p=0.05). In conclusion, some biochemical parameters (CAT, GSH, MDA, Vit C, and TP) of Simmental cow’s milk and milk cells were evaluated and discussed in the present study. It is thought that udder health will be positively affected by increasing the antioxidant capacity of milk cells.

Keywords

CAT, GSH, MDA, Vit C, Simmental milk cell

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