Ameliorative Effects of Curcumin on Aflatoxin B1-Induced Nephrotoxicity in Wistar-Albino Rats

Year 2022, , 139 - 145, 30.12.2022

Durmuş Hatipoğlu , Ercan Keskin

https://doi.org/10.31196/huvfd.1093603

Cited By: 2

Abstract

Mycotoxins exposed through food can lead to organ dysfunction and even failure. The number of studies on renal failure induced by aflatoxin B1 (AFB1) is limited. The purpose of this trial was to examine the effect of AFB1 on the kidney and whether its possible deleterious effect could be prevented by curcumin, a traditionally used and economical antioxidant. 38 rats were divided into five groups, group I represented the control, while the others were named dimethylsulfoxide, curcumin, AFB1, and AFB1 + Curcumin, respectively. Plasma samples were taken from each group after 60 days. Then, BUN, uric acid, and creatinine levels were determined by the ELISA method. Data were analyzed statistically. Bodyweight gain at the end of the study was the least in the group administered AFB1. Also, BUN, uric acid, and creatinine levels were higher in this group than in the other groups. Concomitant administration of AFB1 and curcumin improved both body weight gain and BUN, uric acid, and creatinine levels. Therefore, curcumin can be considered as a low cost-high efficacy renal protective agent in the prevention of renal failure caused by mycotoxins, especially AFB1.

Keywords

Aflatoxin B1, Curcumin, Kidney injury, Rat

Supporting Institution

Selcuk University Scientific Research Projects Coordination Office

Project Number

18102035

Kurkuminin Aflatoksin B1 ile İndüklenen Wistar-Albino Ratlarda Nefrotoksisite Üzerindeki İyileştirici Etkileri

Abstract

Gıda yoluyla maruz kalınan mikotoksinler, organ fonksiyon bozukluğuna ve hatta yetmezliğe yol açabilir. Aflatoksin B1'in (AFB1) neden olduğu böbrek yetmezliği ile ilgili çalışmaların sayısı sınırlıdır. Bu araştırmanın amacı, AFB1'in böbrek üzerindeki etkisini ve olası zararlı etkisinin geleneksel olarak kullanılan ve ekonomik bir antioksidan olan kurkumin tarafından önlenip önlenemeyeceğini incelemekti. 38 rat beş gruba ayrıldı, grup I kontrolü temsil ederken diğerleri sırasıyla dimetilsülfoksit (D), kurkumin (Cur), AFB1 (AF) ve AFB1 + Kurkumin (AF+Cur) olarak adlandırıldı. Her gruptan 60 gün sonra plazma örnekleri alındı. Daha sonra ELISA yöntemi ile BUN, ürik asit ve kreatinin düzeyleri belirlendi. Veriler istatistiksel olarak analiz edildi. Çalışmanın sonunda vücut ağırlığı artışı en az AFB1 uygulanan gruptaydı. Ayrıca bu grupta BUN, ürik asit ve kreatinin seviyeleri diğer gruplara göre daha yüksekti. AFB1 ve kurkuminin birlikte uygulanması hem vücut ağırlığı artışını hem de BUN, ürik asit ve kreatinin düzeylerini iyileştirdi. Bu nedenle kurkumin, mikotoksinlerin, özellikle AFB1'in neden olduğu böbrek yetmezliğinin önlenmesinde düşük maliyetli, yüksek etkili bir böbrek koruyucu ajan olarak kabul edilebilir.

Keywords

Aflatoksin B1, Kurkumin, Böbrek Hasarı, Rat

Project Number

18102035

References

• Abdel-Daim MM, Abdeen, A, Jalouli, M, Abdelkader, A, Megahed, A, Alkahtane, A, Almeer, R, Alhoshani, NM, Al-Johani, NS, Alkahtani, S, Aleya, L 2021: Fucoidan supplementation modulates hepato-renal oxidative stress and DNA damage induced by aflatoxin B1 intoxication in rats. Sci Total Environ, 768,144781.
• Abdel-Hamid AA, Firgany, AE-DL 2015: Vitamin E supplementation ameliorates aflatoxin B1-induced nephrotoxicity in rats. Acta Histochem, 117,767-779.
• Ak T, Gülçin, İ 2008: Antioxidant and radical scavenging properties of curcumin. Chem Biol Interact, 174,27-37.
• Ali FA, Abdel-Maksoud, FM, Abd Elaziz, HO, Al-Brakati, A, Elmahallawy, EK 2021: Descriptive Histopathological and Ultrastructural Study of Hepatocellular Alterations Induced by Aflatoxin B1 in Rats. Animals, 11.
• Allegra A, Innao, V, Russo, S, Gerace, D, Alonci, A, Musolino, C 2017: Anticancer Activity of Curcumin and Its Analogues: Preclinical and Clinical Studies. Cancer Invest, 35,1-22.
• Andretta I, Kipper, M, Lehnen, CR, Lovatto, PA 2012: Meta-analysis of the relationship of mycotoxins with biochemical and hematological parameters in broilers. Poult Sci, 91,376-382.
• Asplin F, Carnaghan, R 1961: The toxicity of certain groundnut meals for poultry with special reference to their effect on ducklings and chickens. Vet Rec, 73,1215-1219.
• Ates MB, Ortatatli, M 2021: The effects of Nigella sativa seeds and thymoquinone on aflatoxin phase-2 detoxification through glutathione and glutathione-S-transferase alpha-3, and the relationship between aflatoxin B1-DNA adducts in broilers. Toxicon, 193,86-92.
• Ates MB, Ortatatli, M, Oguz, H, Ozdemir, O, Terzi, F, Ciftci, MK, Hatipoglu, F 2022: The ameliorative effects of Nigella sativa, thymoquinone, and bentonite against aflatoxicosis in broilers via AFAR and Nrf2 signalling pathways, and down-regulation of caspase-3. Br Poult Sci,1-8.
• Ateş MB, Ortatatlı, M 2020: Protective effect of nigella sativa and thymoquinone on relative liver weight increase caused by aflatoxin in broilers. Eurasian Journal of Veterinary Sciences, 36,107-114.
• Brase S, Glaser, F, Kramer, C, Lindner, S, Linsenmeier, A, Masters, K, Meister, A, Ruff, B, Zhong, S 2013: The chemistry of mycotoxins [Progress in the Chemistry of Organic Natural Products, Volume 97].
• Chen X, Horn, N, Cotter, PF, Applegate, TJ 2014: Growth, serum biochemistry, complement activity, and liver gene expression responses of Pekin ducklings to graded levels of cultured aflatoxin B1. Poult Sci, 93,2028-2036.
• Cimbalo A, Alonso-Garrido, M, Font, G, Manyes, L 2020: Toxicity of mycotoxins in vivo on vertebrate organisms: A review. Food Chem Toxicol, 137,111161.
• Dei Cas M, Ghidoni, R 2019: Dietary Curcumin: Correlation between Bioavailability and Health Potential. Nutrients, 11,2147.
• Dönmez N, Dönmez, H, Keskin, E, Kısadere, I 2012: Effects of aflatoxin on some haematological parameters and protective effectiveness of esterified glucomannan in Merino rams. The Scientific World Journal, 2012.
• Dönmez N, Keskin, E 2008: The effects of aflatoxin and glucomannan on some antioxidants and biochemical parameters in rabbits. Eurasian Journal of Veterinary Sciences, 23,31-35.
• El-Bahr SM 2015: Effect of Curcumin on Hepatic Antioxidant Enzymes Activities and Gene Expressions in Rats Intoxicated with Aflatoxin B1. Phytother Res, 29,134-140.
• El-Mahalaway AM 2015: Protective effect of curcumin against experimentally induced aflatoxicosis on the renal cortex of adult male albino rats: a histological and immunohisochemical study. Int J Clin Exp Pathol, 8,6019-6030.
• El-Rahman S, Al-Jameel, S 2014: Protection of curcumin and curcumin nanoparticles against cisplatin induced nephrotoxicity in male rats. Sch. Acad. J. Biosci, 2,214-223.
• El‐Mekkawy HI, Al‐Kahtani, MA, Shati, AA, Alshehri, MA, Al‐Doaiss, AA, Elmansi, AA, Ahmed, AE 2020: Black tea and curcumin synergistically mitigate the hepatotoxicity and nephropathic changes induced by chronic exposure to aflatoxin‐B1 in Sprague–Dawley rats. J Food Biochem, 44,e13346.
• Engin AB, Engin, A 2019: DNA damage checkpoint response to aflatoxin B1. Environ Toxicol Pharmacol, 65,90-96.
• Eraslan G, Sarıca, ZS, Bayram, LÇ, Tekeli, MY, Kanbur, M, Karabacak, M 2017: The effects of diosmin on aflatoxin-induced liver and kidney damage. Environmental Science and Pollution Research, 24,27931-27941.
• FAO 2013: Mycotoxins. Food safety and quality. Food and Agriculture Organization of the United Nations.
• Fung F, Clark, RF 2004: Health Effects of Mycotoxins: A Toxicological Overview. J Toxicol Clin Toxicol, 42,217-234.
• Garcia-Cohen E-C, Marin, J, Diez-Picazo, LD, Baena, AB, Salaices, M, Rodriguez-Martinez, MA 2000: Oxidative Stress Induced by Butyl Hydroperoxide Causes Vasoconstriction in the Aorta from Hypertensive and Aged Rats: Role of Cyclooxygenase-2 Isoform. J Pharmacol Exp Ther, 293,75-81.
• García-Niño WR, Pedraza-Chaverrí, J 2014: Protective effect of curcumin against heavy metals-induced liver damage. Food Chem Toxicol, 69,182-201.
• Gowda NKS, Ledoux, DR, Rottinghaus, GE, Bermudez, AJ, Chen, YC 2008: Efficacy of Turmeric (Curcuma longa), Containing a Known Level of Curcumin, and a Hydrated Sodium Calcium Aluminosilicate to Ameliorate the Adverse Effects of Aflatoxin in Broiler Chicks. Poult Sci, 87,1125-1130.
• Grosman ME, Elías, MM, Comin, EJ, Garay, EAR 1983: Alterations in renal function induced by aflatoxin B1 in the rat. Toxicol Appl Pharmacol, 69,319-325.
• Guo H-W, Chang, J, Wang, P, Yin, Q-Q, Liu, C-Q, Xu, X-X, Dang, X-W, Hu, X-F, Wang, Q-L 2021: Effects of compound probiotics and aflatoxin-degradation enzyme on alleviating aflatoxin-induced cytotoxicity in chicken embryo primary intestinal epithelium, liver and kidney cells. AMB Express, 11,35.
• Gupta SC, Patchva, S, Aggarwal, BB 2013: Therapeutic roles of curcumin: lessons learned from clinical trials. The AAPS journal, 15,195-218.
• Hosseini A, Hosseinzadeh, H 2018: Antidotal or protective effects of Curcuma longa (turmeric) and its active ingredient, curcumin, against natural and chemical toxicities: A review. Biomed Pharmacother, 99,411-421.
• Karabacak M, Eraslan, G, Kanbur, M, Sarıca, ZS 2015: Effects of Tarantula cubensis D6 on aflatoxin-induced injury in biochemical parameters in rats. Homeopathy, 104,205-210.
• Khan R, Ghazali, FM, Mahyudin, NA, Samsudin, NIP 2021: Aflatoxin Biosynthesis, Genetic Regulation, Toxicity, and Control Strategies: A Review. Journal of Fungi, 7,606. Khoursandi L, Ourazizadeh, M 2008: Protective effect of Curcuma longa extract on acetaminophen induced nephrotoxicity in mice.
• Kim KS, Lim, H-J, Lim, JS, Son, JY, Lee, J, Lee, BM, Chang, S-C, Kim, HS 2018: Curcumin ameliorates cadmium-induced nephrotoxicity in Sprague-Dawley rats. Food Chem Toxicol, 114,34-40.
• Kubena LF, Huff, WE, Harvey, RB, Yersin, AG, Elissalde, MH, Witzel, DA, Giroir, LE, Phillips, TD, Petersen, HD 1991: Effects of a Hydrated Sodium Calcium Aluminosilicate on Growing Turkey Poults During Aflatoxicosis1. Poult Sci, 70,1823-1830.
• Kumar N, Rana, R, Rana, DS, Gupta, A, Sachdeva, MP 2021: Donor-Derived Cell-Free DNA to Diagnose Graft Rejection Post-Transplant: Past, Present and Future. Transplantology, 2,348-361.
• McLean M, Dutton, MF 1995: Cellular interactions and metabolism of aflatoxin: An update. Pharmacol Ther, 65,163-192.
• Mollenhauer HH, Corrier, DE, Huff, WE, Kubena, LF, Harvey, RB, Droleskey, RE 1989: Ultrastructure of hepatic and renal lesions in chickens fed aflatoxin. Am J Vet Res, 50,771-777.
• Na L-X, Li, Y, Pan, H-Z, Zhou, X-L, Sun, D-J, Meng, M, Li, X-X, Sun, C-H 2013: Curcuminoids exert glucose-lowering effect in type 2 diabetes by decreasing serum free fatty acids: a double-blind, placebo-controlled trial. Mol Nutr Food Res, 57,1569-1577.
• Naaz F, Abdin, MZ, Javed, S 2014: Protective effect of esculin against prooxidant aflatoxin B1-induced nephrotoxicity in mice. Mycotoxin Research, 30,25-32.
• Nada S, Amra, H, Deabes, M, Omara, E 2010: Saccharomyces cerevisiae and probiotic bacteria potentially inhibit aflatoxins production in vitro and in vivo studies. Int. J. Toxicol, 8,32.
• Nasrollahzadeh A, Mokhtari, S, Khomeiri, M, Saris, P 2022: Mycotoxin detoxification of food by lactic acid bacteria. International Journal of Food Contamination, 9,1.
• Oguz H 2011: A review from experimental trials on detoxification of aflatoxin in poultry feed. Eurasian Journal of Veterinary Sciences, 27,1-12.
• Oguz H, Kurtoglu, V 2000: Effect of clinoptilolite on performance of broiler chickens during experimental aflatoxicosis. Br Poult Sci, 41,512-517.
• Ortatatli M, Oğuz, H, Hati̇poğlu, F, Karaman, M 2005: Evaluation of pathological changes in broilers during chronic aflatoxin (50 and 100 ppb) and clinoptilolite exposure. Res Vet Sci, 78,61-68.
• Parvathy KS, Negi, PS, Srinivas, P 2009: Antioxidant, antimutagenic and antibacterial activities of curcumin-β-diglucoside. Food Chem, 115,265-271.
• Quezada T, Cuellar, H, Jaramillo-Juarez, F, Valdivia, A, Reyes, J 2000: Effects of aflatoxin B1 on the liver and kidney of broiler chickens during development. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 125,265-272.
• Rai M, Varma, A, 2010. Mycotoxins in food, feed and bioweapons. Springer.
• Saini SS, Kaur, A 2012: Aflatoxin B1: Toxicity, characteristics and analysis: Mini review. Global Advanced Research Journal of Chemistry and Material Science, 1,063-070.
• Sasikumar B, George, KJ, Saji, K, Zachariah, TJ 2005: Two new high yielding, high curcumin, turmeric (Curcuma longa L.) varieties-'IISR Kedaram'and'IISR Alleppey Supreme'. Journal of Spices and Aromatic Crops, 14,71-74.
• Sharma V, Sharma, C, Sharma, S 2011: Influence of Curcuma Longa and Curcumin on blood profile in mice subjected to aflatoxin B1. International Journal of Pharmaceutical Sciences and Research, 2,1740.
• Sun W, Wang, S, Zhao, W, Wu, C, Guo, S, Gao, H, Tao, H, Lu, J, Wang, Y, Chen, X 2017: Chemical constituents and biological research on plants in the genus Curcuma. Crit Rev Food Sci Nutr, 57,1451-1523.
• Tang L, Guan, H, Ding, X, Wang, J-S 2007: Modulation of aflatoxin toxicity and biomarkers by lycopene in F344 rats. Toxicol Appl Pharmacol, 219,10-17.
• Ugur S, Ulu, R, Dogukan, A, Gurel, A, Yigit, IP, Gozel, N, Aygen, B, Ilhan, N 2015: The renoprotective effect of curcumin in cisplatin-induced nephrotoxicity. Ren Fail, 37,332-336.
• Valdivia AG, Martínez, A, Damián, FJ, Quezada, T, Ortíz, R, Martínez, C, Llamas, J, Rodríguez, ML, Yamamoto, L, Jaramillo, F, Loarca-Piña, MG, Reyes, JL 2001: Efficacy of N-Acetylcysteine to Reduce the Effects of Aflatoxin B1 Intoxication in Broiler Chickens1. Poult Sci, 80,727-734.
• Wangikar PB, Dwivedi, P, Sinha, N, Sharma, AK, Telang, AG 2005: Teratogenic effects in rabbits of simultaneous exposure to ochratoxin A and aflatoxin B1 with special reference to microscopic effects. Toxicology, 215,37-47.
• Yavuz O, Özdemir, Ö, Ortatatli, M, Atalay, B, Hatipoglu, F, Terzi, F 2017: The Preventive Effects of Different Doses of Glucomannan on Experimental Aflatoxicosis in Japanese Quails. Revista Brasileira de Ciência Avícola, 19,409-416.
• Yu K, Zhang, J, Cao, Z, Ji, Q, Han, Y, Song, M, Shao, B, Li, Y 2018: Lycopene attenuates AFB1-induced renal injury with the activation of the Nrf2 antioxidant signaling pathway in mice. Food Funct, 9,6427-6434.