Immunohistochemical Alterations of Syndecan-1 in Sheep Liver with Cystic Echinococcosis

Year 2023, , 141 - 145, 22.12.2023

Nihat Yumuşak

https://doi.org/10.31196/huvfd.1310945

Abstract

Cystic echinococcosis is formed by the larval forms of Echinococcus granulosus, causes health problems in humans and different species of animals worldwide. There is still limited information about the pathophysiological response to cystic echinococcosis in the liver. Syndecan-1 (Sdc1) is a cell surface proteoglycan of the liver. It has a crucial role in the pathophysiology of various liver diseases. This study aimed to investigate the immunohistochemical expression of Sdc1 in the liver with cystic echinococcosis in sheep. A total number of 51 liver tissue samples with cystic echinococcosis and ten healthy livers were examined. The tissue samples were stained with hematoxylin-eosin (HE) for histopathological examinations. Sdc1 was determined in the same liver tissues by immunohistochemistry. Infected liver tissues mainly showed severe inflammatory reactions, congestion, diffuse degeneration, and necrosis of hepatocytes around the cysts. The results indicated severe Sdc1-positive staining in hepatocytes around the cysts. Liver tissues of the control group showed relatively mild immunopositive reactions. This study determined that the Sdc1 significantly increased in the sheep livers with cystic echinococcosis infection.

Keywords

echinococcosis, liver, sheep, syndecan-1

Thanks

The author would like to thank Prof.Dr. Mehtap GUL ALTAS for contribute to parasitological procedures during the course of the study.

Kistik Ekinokokkozlu Koyun Karaciğerlerinde Sindekan-1’in İmmünohistokimyasal Değişiklikleri

Abstract

Karaciğer kistik ekinokokkozisi Echinococcus granulosus’un larval formları tarafından oluşturulur. Dünya çapında insanlarda ve farklı hayvan türlerinde sağlık problemlerine neden olur. Kistik ekinokokkozisin patofizyolojisi hakkında hala sınırlı bilgi mevcuttur. Sindekan-1 (Sdc1) karaciğer hücre yüzey proteoglikanıdır. Farklı karaciğer hastalıklarının patofizyolojisinde önemli rolleri vardır. Bu çalışmada kistik ekinokokkozisli koyun karaciğerlerinde Sdc1 salınımındaki değişiklerin immünohistokimyasal olarak belirlenmesi amaçlanmıştır. Toplam 51 hidatid kistli karaciğer dokusu ve 10 sağlıklı karaciğer dokusu incelendi. Histopatolojik incelemeler amacıyla doku örnekleri hematoksilen-eosin (HE) ile boyandı. Aynı karaciğer dokularında Sdc1 immünohistokimyasal olarak belirlendi. Enfekte karaciğer dokularında başlıca şiddetli yangısal reaksiyon, konjesyon ve kist duvarları çevresindeki hepatositlerde yaygın dejenerasyon ve nekrozlar görüldü. Kist duvarları çevresindeki hepatositlerde şiddetli Sdc1 immünpozitif reaksiyon belirlendi. Kontrol grubuna ait karaciğer dokuları ise hafif immünopozitif reaksiyon gösterdi. Bu çalışmada ekinokokkozisli koyun karaciğer dokularında Sdc1 salınımın belirgin şekilde arttığı tespit edildi.

Keywords

ekinokokkozis, karaciğer, koyun, sindekan-1

References

• Afratis NA, Nikitovic D, Multhaupt HA, Theocharis AD, Couchman JR, Karamanos NK, 2017. Syndecans key regulators of cell signaling and biological functions, FEBSJ, 284, 27–41.
• Al Malki J, Ahmed N, 2022: Epidemiological and histomorphic studies in sheep infected with hydatid cyst in Taif area. Saudi J Biol Sci, 29, 886-893.
• Aquino RS, Hayashida A, Park PW, 2020: Host syndecan-1 promotes listeriosis by inhibiting intravascular neutrophil extracellular traps. PLoS Pathogens, 16 (5), e1008497.
• Beiting DP, Park PW, Appleton JA, 2006: Synthesis of syndecan-1 by skeletal muscle cells is an early response to infection with Trichinella spiralis but is not essential for nurse cell development. Infect Immun, 74 (3), 1941-1943.
• Bhanot P, Nussenzweig V, 2002: Plasmodium yoelii sporozoites infect Syndecan-1 deficient mice. Mol Biochem Parasitol, 2 (123), 143-144.
• Budke CM, 2006: Global socioeconomic impact of cystic echinococcosis. Emerg Infect Dis, 12, 296–303.
• Cabrera PA, Haran G, Benavidez V et al., 1995: Transmission dynamics of Echinococcus granulosus, Taenia hydatigena and Taenia ovis in sheep in Uruguay. Int J Parasitol, 25, 807–813.
• Couchman JR, 2021: Syndecan-1 (CD138), carcinomas and EMT. Int J Mol Sci, 22, 4227.
• Craig PS, McManus DP, Lightowlers MW et al., 2007: Prevention and control of cystic echinococcosis. Lancet Infect Dis, 7, 385-394.
• Derda M, Wandurska-Nowak E, Hadas E, 2004: Changes in the level of antioxidants in the blood from mice infected with Trichinella spiralis. Parasitol Res, 93, 207–210.
• Eckert J, Deplazes P, 2004: Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev, 17, 107-135.
• Etewa S, Sarhan M, Moawad H, Mohammad S, Samir M, Kandil A, Mostafa E, 2021: Behavior and neuropsychiatric changes in experimental chronic toxoplasmosis: Histopathological and immunohistochemical studies. Parasitologists United Journal, 14 (2), 183-192.
• Gerazounis M, Athanassiadi K, Metaxas E, Athanassiou M, Kalantzi N, 2002: Bronchobiliary fistulae due to echinococcosis. Eur J Cardiothorac Surg, 22 (2), 306-308.
• Gopal S, Arokiasamy S, Pataki C, Whiteford JR, Couchman JR, 2021: Syndecan receptors: pericellular regulators in development and inflammatory disease. Open Biol, 11, 200377.
• Heidarpour M, Mohri M, Borji H, Moghdass E, 2012: Oxidative stress and trace elements in camel (Camelus dromedarius) with liver cystic echinococcosis. Vet Parasitol, 187, 459–463.
• Ibrahim SEA, Gameel AA, 2014: Pathological, histochemical and Immunohistochemical studies of lungs and livers of cattle and sheep infected with hydatid disease. In: Proceedings of 5th annual conference-agricultural and veterinary research–February 2014, Khartoum, Sudan, 2, 1–17.
• Jacobs W, Bogers J, Van Marck E, 1999: Distinct B-cell populations are present in hepatic and intestinal Schistosoma mansoni granulomas. Acta Gastroenterol Belg, 62 (2):178-81.
• Kebede N, Mitiku A, Tilabun G, 2009: Hydatidosis of slaughtered animals in Bahir Dar abattoir, northwestern Ethiopia. Trop Anim Health Prod, 41, 43-50.
• Khadidja H, Achour Y, Houcin B, Vasile C, 2014: Histological Appearance of Echinococcus Granulosus in the Camel Species in Algeria. Bulletin UASVM Vet Med, 71, 79–84
• Kılıç E, Yazar S, Başkol G, Artiş T, Ersayit D, 2010: Antioxidant and nitric oxide status in patients diagnosed with Echinococcus granulosus. Afr J Microbiol Res, 4, 2439- 2443.
• Kim CW, Goldberger OA, Gallo RL, Bernfield M, 1994: Members of the syndecan family of heparan sulfate proteoglycans are expressed in distinct cell-, tissue-, and development-specific patterns, Mol Biol Cell, 5, 797–805.
• Matsumoto A, Ono M, Fujimoto Y, Gallo RL, Bernfield M, Kohgo Y, 1997: Reduced expression of syndecan-1 in human hepatocellular carcinoma with high metastatic potential. Int J Cancer, 74, 482-491.
• McManus DP, Zhang W, Li J, Bartley PB, 2003: Echinococcosis. Lancet, 362, 1295–1304.
• Mert H, Yaşar S, Mert N, 2019: Investigation of TAS and TOS levels in sheep with hydatid cyst. EurasianJHS, 2 (4), 164-168.
• Nam EJ, Hayashida K, Aquino RS, Couchman JR, Kozar RA, Liu J, Park PW, 2017: Syndecan‐1 limits the progression of liver injury and promotes liver repair in acetaminophen‐induced liver injury in mice. Hepatology, 66, 1601-1615
• Palaiologou M, Delladetsima I, Tiniakos D, 2014: CD138 (syndecan-1) expression in health and disease, Histol Histopathol, 29, 177-189.
• Regos E, Karászi K, Reszegi A, Kiss A, Schaff Z, Baghy K, Kovalszky I, 2020: Syndecan-1 in liver diseases. Pathol Oncol Res, 26, 813-819.
• Reszegi A, Tátrai P, Regős E, Kovalszky I, Baghy K, 2022: Syndecan-1 in liver pathophysiology. Am J Physiol Cell Physiol, 323, 289-294.
• Rogan MT, Craig PS, 1997: Immunology of Echinococcus granulosus infections. Acta Tropica, 67, 7-17.
• Roskams T, Rosenbaum J, De Vos R, David G, Desmet V, 1996: Heparan sulfate proteoglycan expression in chronic cholestatic human liver diseases. Hepatology, 24, 524-532.
• Saleh MA, 2008: Circulating oxidative stress status in desert sheep naturally infected with Fasciola hepatica. Vet Parasitol, 154, 262–269.
• Serefettin M, Merih G, Tulay C, Astarcioglu H, 2003: The Pathology of Echinococcosis and the Current Echinococcosis Problem in Western Turkey (A Report of Pathologic Features in 80 Cases). Turk J Med Sci, 33, 369-374.
• Shoulah SA, Gaballa MM, Marawan MA, Saqr SA, Abdelhady A, Alzahrani HA, Wakid M, Al-Jabr O, Selim A, 2023: Pathological Findings and Oxidative Stress Status Associated with Hydatidosis in Dromedary Camels. Vet Sci, 10, 74.
• Singh BB, Sharma R, Sharma JK, Mahajan V, Gill JPS, 2014: Histopathological changes associated with E. Granulosus echinococcosis in food producing animals in Punjab (India). J Parasit Dis, 40, 1–3
• Teng YHF, Aquino RS, Park PW, 2012: Molecular functions of syndecan-1 in disease. Matrix Biol, 31, 3-16.
• Widdicombe J, Basáñez MG, Entezami M, Jackson D, Larrieu E, Prada JM, 2022: The economic evaluation of Cystic echinococcosis control strategies focused on zoonotic hosts: A scoping review. PLoS Negl Trop Dis, 16 (7), e0010568.
• Yilmaz Y, Eren F, Colak Y, Senates E, Celikel CA, Imeryuz N, 2012: “Hepatic expression and serum levels of syndecan 1 (CD138) in patients with nonalcoholic fatty liver disease”. Scand J Gastroenterol, 47 (12), 1488-1493.
• Yumusak N, Filikci K, 2022: Immunohistochemical Evaluation of Syndecan-1 Expression in the Liver of Small Ruminants with Natural Liver Fluke Infection. Harran Univ Vet Fak Derg, 11, 73-77.
• Zvibel I, Halfon P, Fishman S, Penaranda G, Leshno M, Or AB, Halpern Z, Oren R, 2009: Syndecan 1 (CD138) serum levels: a novel biomarker in predicting liver fibrosis stage in patients with hepatitis C. Liver Int, 29, 208-212.