Kistik Ekinokokkozlu Koyun Karaciğerlerinde Sindekan-1’in İmmünohistokimyasal Değişiklikleri

Abstract

Karaciğer kistik ekinokokkozisi Echinococcus granulosus’un larval formları tarafından oluşturulur. Dünya çapında insanlarda ve farklı hayvan türlerinde sağlık problemlerine neden olur. Kistik ekinokokkozisin patofizyolojisi hakkında hala sınırlı bilgi mevcuttur. Sindekan-1 (Sdc1) karaciğer hücre yüzey proteoglikanıdır. Farklı karaciğer hastalıklarının patofizyolojisinde önemli rolleri vardır. Bu çalışmada kistik ekinokokkozisli koyun karaciğerlerinde Sdc1 salınımındaki değişiklerin immünohistokimyasal olarak belirlenmesi amaçlanmıştır. Toplam 51 hidatid kistli karaciğer dokusu ve 10 sağlıklı karaciğer dokusu incelendi. Histopatolojik incelemeler amacıyla doku örnekleri hematoksilen-eosin (HE) ile boyandı. Aynı karaciğer dokularında Sdc1 immünohistokimyasal olarak belirlendi. Enfekte karaciğer dokularında başlıca şiddetli yangısal reaksiyon, konjesyon ve kist duvarları çevresindeki hepatositlerde yaygın dejenerasyon ve nekrozlar görüldü. Kist duvarları çevresindeki hepatositlerde şiddetli Sdc1 immünpozitif reaksiyon belirlendi. Kontrol grubuna ait karaciğer dokuları ise hafif immünopozitif reaksiyon gösterdi. Bu çalışmada ekinokokkozisli koyun karaciğer dokularında Sdc1 salınımın belirgin şekilde arttığı tespit edildi.

Keywords

• ekinokokkozis
• karaciğer
• koyun
• sindekan-1

Immunohistochemical Alterations of Syndecan-1 in Sheep Liver with Cystic Echinococcosis

Abstract

Cystic echinococcosis is formed by the larval forms of Echinococcus granulosus, causes health problems in humans and different species of animals worldwide. There is still limited information about the pathophysiological response to cystic echinococcosis in the liver. Syndecan-1 (Sdc1) is a cell surface proteoglycan of the liver. It has a crucial role in the pathophysiology of various liver diseases. This study aimed to investigate the immunohistochemical expression of Sdc1 in the liver with cystic echinococcosis in sheep. A total number of 51 liver tissue samples with cystic echinococcosis and ten healthy livers were examined. The tissue samples were stained with hematoxylin-eosin (HE) for histopathological examinations. Sdc1 was determined in the same liver tissues by immunohistochemistry. Infected liver tissues mainly showed severe inflammatory reactions, congestion, diffuse degeneration, and necrosis of hepatocytes around the cysts. The results indicated severe Sdc1-positive staining in hepatocytes around the cysts. Liver tissues of the control group showed relatively mild immunopositive reactions. This study determined that the Sdc1 significantly increased in the sheep livers with cystic echinococcosis infection.

Keywords

• echinococcosis
• liver
• sheep
• syndecan-1

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