Investigation of Ivermectin Susceptibility in Kangal and Akbaş Dogs via MDR1 Gene Mutation

Year 2024, , 1 - 7, 25.06.2024

Emine Baydan , Bengi Cınar Kul , Mustafa Yenal Akkurt , Ebru Yıldırım , Emre Arslanbaş , Farah Gönül Aydın

https://doi.org/10.31196/huvfd.1348411

Abstract

This research aimed to investigate the sensitivity to the drug (ivermectin) in Kangal and Akbaş breed dogs, which are dog breeds native to Turkey, via the MDR1 gene mutation.
For the research, blood, hair and intraoral swap samples were taken from 30 Kangal and 20 Akbaş breed dogs (male-female mixed, adult) with ethical permission and approval forms. Kangal dog samples were taken from the dogs bred in the farms in Sivas center and Kangal village, and Akbaş dog samples were taken from the dogs bred in the farms in the Sivrihisar center, Ankara and Eskişehir regions. The samples taken were evaluated based on polymerase chain reaction (PCR) from the wool and subsequent two-way dideoxy chain termination reaction in the presence of 4 base farm deletions (c.296-299delAGAT) in the 4th exon of the ABCB1 gene that encodes the P-glycoprotein (P-gp) drug transporter protein.
According to the obtained DNA sequence results, the deletion of “AGAT” was not determined in any of the individuals screened. The results of this study, which is preliminary research, showed that Kangal and Akbaş breed dogs are safe in terms of sensitivity to drugs that are set to be absorbed and excreted by the P-gp pump, especially ivermectin. However, it would be useful to repeat the analysis of both breeds with more examples.

Keywords

Akbaş, ivermectin, Kangal, MDR1 mutation, susceptibility

Ethical Statement

This study was approved by the Ankara University Animal Experiments Local Ethics Committee (10.02.2016, 2016-3-21 Number Ethics Committee Decision). In addition, the authors declared that Research and Publication Ethical rules were followed.

Supporting Institution

This work has been supported by Ankara University Scientific Research Projects Coordination Unit under grant number 17B0239002.

Project Number

Ankara University Scientific Research Projects Coordination Unit, grant number 17B0239002.

Thanks

This work has been supported by Ankara University Scientific Research Projects Coordination Unit under grant number 17B0239002.

Kangal ve Akbaş Irkı Köpeklerde MDR1 Gen Mutasyonu Üzerinden İvermektine Duyarlılığın Araştırılması

Abstract

Bu araştırmada, Türkiye’ye özgü iki farklı ırk köpekte (Kangal ve Akbaş) ivermektine duyarlılığın gen mutasyonu (MDR1) üzerinden belirlenmesi amaçlanmıştır.
Araştırma için, etik izni ve onam formu bulunan 30 adet Kangal ve 20 adet Akbaş ırkı köpekten (erkek dişi karışık, erişkin) kan, kıl ve ağız içi svap örneği alınmıştır. Kangal köpek örnekleri Sivas merkez ve Kangal köyünde yetiştirilen çiftliklerdeki köpeklerden, Akbaş köpek örnekleri ise, Sivrihisar merkez, Ankara ve Eskişehir bölgesindeki çiftliklerde yetiştirilen köpeklerden alınmıştır. Alınan örnekler P-glycoprotein (P-gp) drug transporter proteinini kodlayan ABCB1 (MDR1) geninin 4. ekzonunda 4 baz çiftlik delesyonun (c.296-299delAGAT) varlığı yününden Polimeraz Zincir Reaksiyonu (PZR) ve takiben çift yönlü dideoksi zincir sonlanma reaksiyonuna göre değerlendirilmiştir.
Elde edilen DNA dizi sonuçlarına göre “AGAT” delesyonu taranan hiçbir köpekte belirlenmemiştir. Bir ön araştırma niteliğinde olan bu çalışmanın sonuçları, Kangal ve Akbaş ırkı köpeklerin başta ivermektin olmak üzere P-gp pompası ile emilme ve atılmaları ayarlanan ilaçlara karşı duyarlılık yönünden güvende olduklarını göstermiştir. Ancak, her iki ırka ait analizlerin daha çok örnekle tekrarlanması yararlı olacaktır.

Keywords

Akbaş, duyarlılık, ivermektin, Kangal, MDR1 mutasyonu

Project Number

Ankara University Scientific Research Projects Coordination Unit, grant number 17B0239002.

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