Histological Typing and Morphological Characterization of Canine Seminomas

Year 2023, , 180 - 189, 22.12.2023

Gözde Yücel Tenekeci , Arda Selin Tunç

https://doi.org/10.31196/huvfd.1361082

Abstract

The aim of this study was to evaluate seminomas based on cell morphology and histological structures and classify them into subtypes. Thirty canine seminomas were stained with hematoxylin-eosin (HE) and Periodic Acid Schiff (PAS). PAS-positive cases were classified as Classical seminoma (CS) (n:5), while PAS-negative cases were categorized as Spermatocytic seminoma (SS) (n:23). Cases exhibiting both positive and negative staining were evaluated as Spermatocytic/Classical seminoma (n:2). Carcinoma in situ were observed in the cases with Classical seminoma (n:3). The cell morphology and mitotic indices of each case were given in detail. Canine seminomas were classified into three subtypes: intratubular (n:12), diffuse (n:1), and intratubular/diffuse (n:10). As a result, it was thought that SS and CS types could be observed together, and it was observed that the intratubular/diffuse subtype is a third type characterized by its transformation from intratubular to diffuse form.

Keywords

Canine seminoma, histopathology, classical seminoma, spermatocytic seminoma

Köpek seminomlarının histolojik tiplendirilmesi ve morfolojik karakterizasyonu

Abstract

Çalışmanın amacını, seminomları hücre morfolojisi ve histolojik yapılarına göre değerlendirerek alt tiplerine ayırmak oluşturdu. Otuz adet seminom hematoksilen-eozin (HE) ve Periyodik Asit Schiff (PAS) ile boyandı. PAS pozitif vakalar Klasik seminom (CS) (n:5), PAS negatif vakalar ise Spermatositik seminom (SS) (n:23) olarak sınıflandırıldı. Hem pozitif hem de negatif boyanma gösteren vakalar Spermatositik/Klasik seminom (n:2) olarak değerlendirildi. Klasik seminom vakalarında Carcinoma in situ gözlendi (n:3). Her bir vakaya ait hücre morfolojisi ve mitotik indeksleri ayrıntılı olarak verildi. Köpek seminomları üç alt tipe ayrıldı: intratübüler (n:12), diffüz (n:1) ve intratübüler/diffüz (n:10). Sonuç olarak, SS ve CS tiplerinin bir arada görülebileceği düşünüldü ve intratübülerden diffüz forma dönüşümüyle karakterize edilen üçüncü bir tipin intratübüler/diffüz alt türü olduğu gözlendi.

Keywords

Köpek seminomları, histopatoloji, klasik seminom, spermatositik seminom

References

• Agnew DW, MacLachlan NJ, 2017: Tumors of the genital systems. In Meuten DJ, editor. Tumors in Domestic Animals, 5th ed. Wiley, p 689-722.
• Akin I, Avci H, Gulaydin A, Belge A, Yaygingul R, 2013: Bilateral malignant seminoma in two dogs. Kafkas Univ Vet Fak Derg, 19 (Suppl-A): A233-A236.
• Bush JM, Gardiner DW, Palmer JS, Rajpert‐De Meyts E, Veeramachaneni DNR, 2011: Testicular germ cell tumours in dogs are predominantly of spermatocytic seminoma type and are frequently associated with somatic cell tumours. Int J Androl, 34(4pt2), e288-e295.
• Grieco V, Riccardi E, Greppi GF, Teruzzi F, Iermano V, Finazzi M, 2008: Canine testicular tumours: a study on 232 dogs. J Comp Path, 138 (2-3), 86-89.
• Grieco V, Riccardi E, Rondena M, Ciampi V, Finazzi M, 2007: Classical and spermatocytic seminoma in the dog: histochemical and immunohistochemical findings. J Comp Pathol, 137 (1), 41- 46.
• Hernández-Jardón N, Rojas-Castañeda JC, Landero-Huerta D, Reyes-Cruz E, Reynoso-Robles R, Juárez-Mosqueda MDL, Medrano A, Reyes-Delgado F, Vigueras-Villaseñor RM, 2022: Cryptorchidism: The dog as a study model. Front Vet Sci, 9, 935307.
• Kennedy PC, Cullen JM, Edwards JF, Goldschmidt MH, Larsen S, Munson L. 133 and Nielsen S, 1998: Histological Classification of genital tumour in domestic animals, 134.
• Kristensen P, Andersen A, Irgens LM, Bye AS, Vagstad N, 1996: Testicular cancer and parental use of fertilizers in agriculture. Cancer Epidemiol Biomarkers Prev, 5, 3-9.
• Lamb JC, Mark TA, Gladen BC, Allen JW, Moore JA, 1981: Male fertility, sister chromatid exchange, and germ cell toxicity following exposure to mixtures of chlorinated phenoxy acids containing 2, 3, 7, 8‐tetrachlorodibenzo‐p‐dioxin. J Toxicol Environ Health. A Current Issues, 8(5-6), 825-834.
• Maiolino P, Restucci B, Papparella S, Paciello O, De Vico G, 2004: Correlation of nuclear morphometric features with animal and human World Health Organization International Histological Classifications of canine spontaneous seminomas. Vet Pathol, 41(6), 608-611.
• Mostofy FK, Sesterhenn IA, 1998: Histological typing of testis tumours. In: World Health Organization International Histological Classification of Tumours, 2nd ed., World Health Organization, Geneva.
• Nasciment HH, Santos AD, Prant AL, Lamego EC, Tondo LA, Flores MM, Fighera RA, Kommers GD, 2020: Testicular tumors in 190 dogs: Clinical, macroscopic and histopathological aspects. Pesquisa Vet Bras, 40, 525-535.
• Nielsen S, 1998: Histological classifications of tumors of the genital system of domestic animals. In: World Health Organization International Histological Classification of Tumors of Domestic Animals, Vol. IV, Armed Forces Institute of Pathology, Washington D.C., p. 17e18.
• Özsoy ŞY, Kutsal O, 2007: Bir köpekte malign seminom. Ankara Üniv Vet Fak Derg, 54 (1), 55-59.
• Skakkebaek N, Berthelsen J, Giwercman, A, Müller J, 1987: Carcinoma‐in‐situ of the testis: possible origin from gonocytes and precursor of all types of germ cell tumours except spermatocytoma. Int J Androl, 10 (1), 19-28.
• Yumuşak N, Çalışkan M, Kutsal O, 2014: Bilaterally diffuse malignant seminoma in a dog. Ankara Üniv Vet Fak Derg, 61 (2), 151-152.