Gebeliğin Farklı Dönemlerindeki Fare Plasentasının Histolojik ve Histokimyasal Özelliklerinin İncelenmesi

Year 2023, , 166 - 179, 22.12.2023

Seçil Koç , Şadiye Kum

https://doi.org/10.31196/huvfd.1371723

Abstract

Bu çalışmada gebeliğin farklı dönemlerine ait fare plasenta örneklerinin histolojik ve histokimyasal özelliklerinin araştırılması amaçlanmıştır. Bu amaçla gebeliğin dördüncü, onuncu ve on yedinci günlerindeki fare plasenta örnekleri toplanarak parafinde bloklandılar. 50μ ara ile 5μ kalınlığında seri
kesitler alındı. Kesitlere histolojik ve histokimyasal boyama yöntemleri uygulandı. Sonuç olarak gebeliğin
farklı dönemlerine ait fare plasentasının histolojik ve histokimyasal özellikleri belirlendi.

Keywords

Fare, plasenta, histoloji, histokimya

Ethical Statement

This study was performed with the permission of the Experimental Animals Local Ethics Committee in Aydın Adnan Menderes University with (ADU HADYEK) 64583101/2016/017 approval number.

Supporting Institution

This study was supported by Aydın Adnan Menderes University Scientific Research Fund with project number VTF-16004.

Project Number

VTF-16004

Investigation of Histological and Histochemical Features of Mouse Placenta in Different Periods of Pregnancy

Abstract

This study aimed to investigate the histological and histochemical properties of mouse placenta samples from different periods of pregnancy. For this purpose, mouse placenta samples were collected on the fourth, tenth, and seventeenth days of pregnancy and blocked in paraffin. Serial sections of 5μ thickness were taken at 50μ intervals. Histological and histochemical staining methods were applied to the sections. As a result, histological and histochemical characteristics of mouse placenta from different periods of pregnancy were determined.

Keywords

Histochemistry, Histology, Mouse, Placenta

Project Number

VTF-16004

References

• Adams AD, Guedj F, Bianchi DW, 2020: Placental development and function in trisomy 21 and mouse models of Down syndrome: Clues for studying mechanisms underlying atypical development. Placenta, 89(1), 58-66.
• Arvola M, 2001: Immunological aspects of maternal-fetal interactions in mice. Doctoral thesis, comprehensive summary. Acta Universitatis Upsaliensis, 13-33.
• Barreto RSN, Romagnolli P, Fratini F, Mess AM, Miglino MA, 2019: Mouse placental scaffolds: a three-dimensional environment model for recellularization. J Tissue Engin, 10, 1-11.
• Chavatte-Palmer P, Tarrade A, 2016: Placentation in different mammalian species. In Ann D'endocrin, 77(2), 67-74.
• Culling CFA, Allison RT, Bar WT, 1985: Cellular Pathology Techniques. Butterworth & Co. (Publishers) Ltd. 113,217,232,233,238.
• Deb KF, Paria BC, 2006: Methodologies to study implantation in mice. In: Soares MJ, Hunt MJ (Eds) Placenta and Trophoblast. New Jersey Springer, 470p, 9-34.
• Edwards AK, Janzen-Pang J, Peng A, Tayade C, Carniato A, Yamada AT, Patricia DA, Tse D, 2014: Microscopic Anatomy of the Pregnant Mouse Uterus Throughout Gestation. In: Croy BA, Yamada AT, DeMayo FJ, Adamson SL(eds.), The Guide to Investigation of Mouse Pregnancy, Academic Press, Boston, 43-69. Enders AC, Blankenship TN, 1999: Comparative placental structure. Advanced Drug Delivery Reviews, 38(1), 3-15.
• Erşahin A, Hocaoğlı M, Demirer S, Cengiz F, 2016: Kuersetin, streptozotosin uyarımlı diyabetik sıçanlarda metabolik devreleri ve plasenta morfolojisini iyileştirmektedir. Perin Derg, 24(3), 147-55.
• Flores LE, Hildebrandt TB, Kühl AA, Drews B, 2014: Early detection and staging of spontaneous embryo resorption by ultrasound biomicroscopy in murine pregnancy. Repr Biol Endocrin, 12(1), 38.
• Furukawa S, Hayashi S, Usuda K, Abe M, Hagio S, Ogawa I, 2011: Toxicological pathology in the rat placenta. J Toxicol Pathol, 24(2), 95-111.
• Furukawa S, Kuroda Y, Sugiyama A, 2014: A Comparison of the histological structure of the placenta in experimental animals. J Toxicol Pathol, 27(1), 11-18.
• Hafez S, 2017: Comparative Placental Anatomy: Divergent Structures Serving a Common Purpose. Progress in Molecul Biol Trans Sci, 145, 1-28.
• Harem İŞ, Alabay B, 2018: Ratlarda gebeliğin değişik dönemlerinde trofoblastların özellikleri üzerinde histokimyasal ve immunohistokimyasal çalışmalar. Harran Üniv Vet Fak Derg, (Özel Sayı), 1-11.
• Johnson MH, Everitt BJ, 2018: Coitus and fertilization. In: Essential reproduction. John Wiley & Sons, United Kingdom, 145-160.
• Koç S, Kum S, 2022: Investigation of IgG, vimentin, CD45 distribution, and density in mouse placenta at different periods of pregnancy. Turk J Vet Anim Sci, 46,505-516.
• McGeady TA, Quinn PJ, FitzPatrick ES, Ryan MT, Kilroy D, Lonergan P, 2017: Fertilization In: Veterinary Embryology, University of Minnesota, 400, 26-100.
• Mutluay D, 2019: Preimplantasyon sürecinde sıçan uterus dokusunda meydana gelen değişikliklerin ışık mikroskobik düzeyde incelenmesi. MAE Sağlık Bilimleri Enst. Derg, 3(2), 43-53.
• Özer A, 2007: Veteriner Embriyoloji.Nobel Yayın Dağıtım, Ankara, 27-41.
• Rai A, Cross JC, 2014: Development of the hemochorial maternal vascular spaces in the placenta through endothelial and vasculogenic mimicry. Develop Biol, 387(2), 131-41.
• Rossant J, Cross JC, 2001: Placental development: lessons from mouse mutants. Nature Rev Gen, 2(7), 538. Sinowatz F, 2009: Fertilization. In: Hyttel P, Sinowatz F, Vejlsted M, Betteridge K (eds), Essentials of domestic animal embryology. Elsevier Health Sciences, Canada, 56-68.
• Sur E, Öznurlu Y, Özaydin Y, Çelik İ, Aydın İ, Kadyralieva N, 2015: Gebe farelerde desidua bazalis dokusundaki PAS-pozitif uterus doğal katil hücrelerinin dağılımı. Kafkas Univ Vet Fak Derg, 21(3), 405-411.
• Tesser RB, Scherholz PLA, Nascimento L, Katz SG, 2010: Trophoblast glycogen cells differentiate early in the mouse ectoplacental cone: putative role during placentation. Histochem Cell Biol, 134, 83-92.
• Tewari V, Tewari A, Bhardwaj N, 2011: Histological and histochemical changes in placenta of diabetic pregnant females and its comparison with normal placenta. Asian Pacific J Trop Dis, 1(1), 1-4.
• Watson ED, Cross JC, 2005: Development of structures and transport functions in the mouse placenta. Physiol, 20, 180-93.
• Wu D, Xu J, Lei J, McLane M, Zijl PC, Burd I, 2018: Dynamic glucose enhanced MRI of the placenta in a mouse model of intrauterine inflammation. Placenta, 69, 86-91.
• Yu Q, Qiu Y, Wang X, Tang J, Liu Y, Mei L, Li M, Yang M, Tang L, Gao H, Zhang Z, Xu W, He Q, 2018: Efficient siRNA transfer to knock down a placenta-specific lncRNA using RGD-modified nano-liposome: A new preeclampsia-like mouse model. Inter J Pharm, 546(1-2), 115-24.
• Zhu H, Xu X, Shi X, Feng Y, Xiong Y, Nan Y, Zhang C, Gao L, Chen Y, Xu D, Wang H, 2019: Activation of autophagy inhibits cadmium-triggered apoptosis in human placental trophoblasts and mouse placenta. Environ Poll, 254(PtA), 112991.