Yersinia ruckeri’ye Karşı Bakteriyofaj İzolasyonu ve Konak Aralığının Belirlenmesi

Year 2026, Volume: 15 Issue: 1, 11 - 19, 27.03.2026

Serhat Murat Alkan , Osman Yaşar Tel , Hakan Kalender

https://doi.org/10.31196/huvfd.1810078

https://izlik.org/JA23FS76KF

Abstract

Yersinia ruckeri'nin neden olduğu enterik kızıl ağız hastalığı, balıklarda önemli ekonomik kayıplara neden olan bakteriyel hastalıklardan biridir. Bakteriyel balık hastalıklarının tedavisinde antibiyotikler kullanılmaktadır. Ancak son yıllarda antibiyotik direncinin artması, alternatif tedavilerin geliştirilmesini zorunlu kılmıştır. Bakteriyofaj tedavisi, antibiyotiklere dirençli bakteriyel enfeksiyonların tedavisinde, antibiyotiklere karşı umut vadeden bir alternatif olarak giderek daha fazla önem kazanmaktadır. Bu çalışmanın amacı, Türkiye ve dünyanın birçok bölgesinde yaygın olarak görülen bir hastalık olan yersiniosise karşı faj izole etmek ve izole edilen fajın konak aralığını belirlemektir. Bu çalışmada, belediye atık sularından bakteriyofaj izolasyonu için zenginleştirme ve çift katmanlı agar yöntemleri kullanılmıştır. Belediye atık sularından bir bakteriyofaj izole edilmiştir. İzole edilen faja, konak genusu ve suşuna göre YP4M adı verilmiştir. Bu fajın konak aralığı, spot test kullanılarak belirlenmiştir. Fajların konakçı aralığını belirlemek için 18 Y. ruckeri izolatı, Lactococcus garvieae (ATCC), Aeromonas hydrophila (ATCC) ve Vibrio anguillarum (ATCC) suşları ile birlikte kullanıldı. YP4M, 18 Y. ruckeri izolatından 3, 4, 8, 9, 13, 14, 15 ve 17 numaralı bakterilere karşı litik aktivite gösterirken, Lactococcus garvieae (ATCC), Aeromonas hydrophila (ATCC) ve Vibrio anguillarum (ATCC) suşlarına karşı litik aktivite göstermedi. Fajların karakterizasyonunun ve faj kokteyllerinin hazırlanmasının, su ürünleri yetiştiriciliğinde önemli bir hastalık olan yersiniosisin kontrolüne önemli katkı sağlayacağı düşünülmektedir.

Keywords

Bakteriyofaj , Yersinia ruckeri , Yersiniosis , Antibiyotik Direnci , Faj Terapisi

Ethical Statement

"Yersinia ruckeri'ye Karşı Faj izolasyonu ve Karakterizasyonu" isimli çalışma 20.05.2016 tarih 106973 sayılı Hayvan Deneyleri Yerel Etik Kurulu Yönergesinin EVDE-HADYEK'in çalışma yöntemi madde 7'nin n) fıkrasına istinaden ETİK KURUL İZNİNE TABİ OLMADIĞINA dair oy birliği ile karar verilmiştir

Supporting Institution

Bu çalışma, Türkiye Cumhuriyeti Tarım ve Orman Bakanlığı, Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü tarafından finanse edilmiştir (TAGEM/HSGYAD/T1/23/A6/P5/6426).

Project Number

TAGEM/HSGYAD/T1/23/A6/P5/6426

Thanks

Bu çalışma, Türkiye Cumhuriyeti Tarım ve Orman Bakanlığı, Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü tarafından finanse edilmiştir

Isolation of Bacteriophage Against Yersinia ruckeri and Determination of Host Range

https://izlik.org/JA23FS76KF

Abstract

Enteric red mouth disease caused by Yersinia ruckeri is one of the bacterial diseases that cause significant economic losses in fish. Antibiotics are used in the treatment of bacterial fish diseases. However, the rise in antibiotic resistance in recent years has necessitated the development of alternative therapies. Bacteriophage therapy is gaining importance as a promising alternative to antibiotics in the treatment of antibioticresistant bacterial infections. The aim of this study was to isolate and characterize bacteriophages against local Yersinia ruckeri isolates obtained from rainbow trout farms in Türkiye, and to determine the host range of the isolated phage using the spot assay method. In this study, enrichment and double-layer agar methods were used to isolate bacteriophage from municipal wastewater. One bacteriophage was isolated from municipal wastewater. The isolated phage was named YP4M based on the host species and strain. The lytic activity of phage YP4M was determined by spot assay against 18 Y. ruckeri isolates, as well as Lactococcus garvieae ATCC 49156, Aeromonas hydrophila ATCC 7966 and Vibrio anguillarum ATCC 43305. YP4M exhibited lytic activity against bacteria numbered 3, 4, 8, 9, 13, 14, 15, and 17 from the 18 Y. ruckeri isolates, but did not exhibit lytic activity against Lactococcus garvieae ATCC 49156, Aeromonas hydrophila ATCC 7966 and Vibrio anguillarum ATCC 43305 strains. It is believed that the characterization of phages and the preparation of phage cocktails will significantly contribute to the control of yersiniosis, an important disease in aquaculture.

Keywords

Antibiotic Resistance , Bacteriophage , Phage Therapy , Yersinia ruckeri , Yersiniosis.

Ethical Statement

This study was conducted at the Elazığ Fisheries Research Institute Directorate. This study was approved by the Local Ethics Committee on Animal Experiments of the Elazığ Veterinary Control Institute (Decision No: 2023/09, dated August 3, 2023), confirming that ethical approval was not required for this research.

Supporting Institution

This study was funded by the General Directorate of Agricultural Research and Policies (TAGEM), Ministry of Agriculture and Forestry of the Republic of Türkiye (project no:TAGEM/HSGYAD/ T1/23/A6/P5/6426).

Project Number

TAGEM/HSGYAD/T1/23/A6/P5/6426

Thanks

This study was funded by the Republic of Turkey, Ministry of Agriculture and Forestry, General Directorate of Agricultural Research and Policies (TAGEM/HSGYAD/T1/23/A6/P5/6426).

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