Efficiency of Mini VIDAS and Culture Technique for Detection of Campylobacter Spp. In Minced Beef

Volume: 4 Number: 2 June 1, 2015
  • Serap Kılıç Altun
  • Semra Gürbüz
  • Sevil Erdenliğ Gürbilek
EN TR

Efficiency of Mini VIDAS and Culture Technique for Detection of Campylobacter Spp. In Minced Beef

Abstract

This study was performed to evaluate the efficiency of the mini VIDAS automated immunoassay system and conventional culture technique to detect Campylobacter species from minced beef enriched in Bolton and Preston broths. A total of 92 minced beef samples were collected from different local markets in Erzurum province in Turkey. Minced beef samples were tested for Campylobacter spp. by the mini VIDAS (bioMérieux, France) in parallel with the culture technique using supplemented agar plates following the enrichment at 42oC for 48 h. Positive results were verified by conventional culture technique with quality control strains (ATCC 33559, ATCC 33560) on selective solid media. Out of 92 minced beef samples, 11 (11.95%) and 10 (10.86%) were positive by the mini VIDAS and culture technique respectively. The sensitivity of mini VIDAS was higher, and provided more accurate results than the conventional culture technique. In addition, both broths can be used efficiently for the enrichment

Keywords

Details

Primary Language

English

Subjects

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Journal Section

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Authors

Serap Kılıç Altun This is me

Semra Gürbüz This is me

Sevil Erdenliğ Gürbilek This is me

Publication Date

June 1, 2015

Submission Date

June 1, 2015

Acceptance Date

-

Published in Issue

Year 2015 Volume: 4 Number: 2

APA
Kılıç Altun, S., Gürbüz, S., & Erdenliğ Gürbilek, S. (2015). Efficiency of Mini VIDAS and Culture Technique for Detection of Campylobacter Spp. In Minced Beef. Harran University Journal of the Faculty of Veterinary Medicine, 4(2), 68-72. https://izlik.org/JA27ZE83LE
AMA
1.Kılıç Altun S, Gürbüz S, Erdenliğ Gürbilek S. Efficiency of Mini VIDAS and Culture Technique for Detection of Campylobacter Spp. In Minced Beef. Harran Univ Vet Fak Derg. 2015;4(2):68-72. https://izlik.org/JA27ZE83LE
Chicago
Kılıç Altun, Serap, Semra Gürbüz, and Sevil Erdenliğ Gürbilek. 2015. “Efficiency of Mini VIDAS and Culture Technique for Detection of Campylobacter Spp. In Minced Beef”. Harran University Journal of the Faculty of Veterinary Medicine 4 (2): 68-72. https://izlik.org/JA27ZE83LE.
EndNote
Kılıç Altun S, Gürbüz S, Erdenliğ Gürbilek S (June 1, 2015) Efficiency of Mini VIDAS and Culture Technique for Detection of Campylobacter Spp. In Minced Beef. Harran University Journal of the Faculty of Veterinary Medicine 4 2 68–72.
IEEE
[1]S. Kılıç Altun, S. Gürbüz, and S. Erdenliğ Gürbilek, “Efficiency of Mini VIDAS and Culture Technique for Detection of Campylobacter Spp. In Minced Beef”, Harran Univ Vet Fak Derg, vol. 4, no. 2, pp. 68–72, June 2015, [Online]. Available: https://izlik.org/JA27ZE83LE
ISNAD
Kılıç Altun, Serap - Gürbüz, Semra - Erdenliğ Gürbilek, Sevil. “Efficiency of Mini VIDAS and Culture Technique for Detection of Campylobacter Spp. In Minced Beef”. Harran University Journal of the Faculty of Veterinary Medicine 4/2 (June 1, 2015): 68-72. https://izlik.org/JA27ZE83LE.
JAMA
1.Kılıç Altun S, Gürbüz S, Erdenliğ Gürbilek S. Efficiency of Mini VIDAS and Culture Technique for Detection of Campylobacter Spp. In Minced Beef. Harran Univ Vet Fak Derg. 2015;4:68–72.
MLA
Kılıç Altun, Serap, et al. “Efficiency of Mini VIDAS and Culture Technique for Detection of Campylobacter Spp. In Minced Beef”. Harran University Journal of the Faculty of Veterinary Medicine, vol. 4, no. 2, June 2015, pp. 68-72, https://izlik.org/JA27ZE83LE.
Vancouver
1.Serap Kılıç Altun, Semra Gürbüz, Sevil Erdenliğ Gürbilek. Efficiency of Mini VIDAS and Culture Technique for Detection of Campylobacter Spp. In Minced Beef. Harran Univ Vet Fak Derg [Internet]. 2015 Jun. 1;4(2):68-72. Available from: https://izlik.org/JA27ZE83LE