Ratlarda Parasetamol ile Oluşturulan Hepatotoksisite Üzerine Taraxacum officinale Etanol Ekstraktının Etkisi

Year 2017, , 11 - 18, 30.06.2017

Esra Aktaş Şenocak Betül Apaydın Yıldırım

https://doi.org/10.31196/huvfd.325672

Cited By: 1

Abstract

Ratlarda parasetamol ile
oluşturulan hepatotoksisite üzerine Taraxacum
officinale etanol ekstraktının kan ve karaciğer dokusundaki bazı
biyokimyasal parametrelere ve karaciğer dokusunun histopatolojisi üzerine etkileri
araştırıldı. Bu çalışmada, 5 aylık yaşta 36 adet Sprague Dawley rat kullanıldı.
Sprague Dawley ratlar rastgele her grupta 6 rat olacak şekilde 6 gruba ayrıldı.
Parasetamol (PARA) verilecek gruplardaki hayvanlar 24 saat aç bırakılıp
ekstrakt verildikten 1 saat sonra 2 g/kg oral olarak (p.o.) PARA verildi.
1.Grup (Kontrol Grubu), %5’lik DMSO intraperitoneal (i.p.), 2.Grup (TOE1
Grubu), 200 mg/kg/gün/i.p. Taraxacum
officinale ekstraktı (TOE) %5’lik DMSO’da çözülerek i.p, 3.Grup (TOE2
Grubu), 250 mg/kg/gün/i.p. Taraxacum
officinale ekstraktı, 4.Grup (PARA Grubu), 2 g/kg/p.o. Parasetamol, 5.Grup
(PTOE1 Grubu), Parasetamol 2 g/kg/gün/p.o. + TOE 200 mg/kg/gün/i.p, 6.Grup
(PTOE2 Grubu), Parasetamol 2 g/kg/gün/p.o. + TOE 250 mg/kg/gün/i.p 8 gün
boyunca uygulandı. Çalışma sonunda ratlardan alınan kan ve karaciğer dokusundan
biyokimyasal ve histopatolojik analizler yapıldı. Parasetamol grubunda plazma AST, ALT, ALP, MDA, CAT ve nitrit
düzeyleri; karaciğer MDA, nitrit ve nitrat düzeyleri kontrol grubuna göre
artarken (P<0.001); plazma GSH (P<0.001), SOD (P<0.001) ve GPx
(P<0.05) düzeyleri; karaciğer GSH, CAT ve SOD (P<0.001) düzeyleri anlamlı
olarak azaldı. Parasetamolün ratlarda oluşturduğu hepatotoksisiteyi Taraxacum officinale bitkisinin toprak
üstü aksamlarından yapılan etanol ekstraktının 200 mg/kg uygulanan miktarının
hepatotoksisiteyi anlamlı olarak azalttığı ve lipid peroksidasyonu önlediği,
TOE 250 mg/kg uygulanan hayvanlarda toksik etki yaptığı tespit edildi. Elde
edilen ekstraktın hepatotoksisite üzerine ve plazma ve karaciğer biyokimyasal
parametreleri üzerine olumlu etkilerinin olduğu saptansa bile karaciğerde
oluşan hasarı düzeltmek için zamana gereksinim duyulduğu kanaatine varıldı.

Keywords

Antioksidan, Ekstrakt, Lipid peroksidasyonu, Parasetamol, Taraxacum officinale

The Effect of Taraxacum officinale Ethanol Extract on Paracetamol Induced Hepatotoxicity in Rats

Abstract

In
this study was investigated on the effects on the some biochemical and histopathological
parameters in plasma and liver tissue of Taraxacum
officinale ethanol extract of
paracetamol induced hepatotoxicity in rats.
In this study was utilized 36 Sprague
Dawley male rats, aged 5 months. Rats were divided 6 groups of 6 rats
each, randomly. Animals of paracetamol administered were fasted 24 hour and 2
g/kg peros (p.o) Paracetamol was given after the 1 hour extract was given.
1.Group Control  5% DMSO intraperitoneal (i.p), 2. Group TOE1 200 mg/kg/day/i.p. Taraxacum officinale extract (TOE) was
dissolved in  5% DMSO in distilled water,
i.p., 3. Group TOE2 250 mg/kg/day/i.p. Taraxacum
officinale extract, 4. Group PARA 2 g/kg/p.o. Paracetamol, 5. Group PTOE1;
Paracetamol 2 g/kg/day/p.o.+ TOE 200 mg/kg/day/i.p, 6. Group PTOE2 Paracetamol
2 g/kg/day/p.o.+ TOE 250 mg/kg/day/i.p were administered for 8 day. At the end
of the study biochemical and histopatological analyses were made from sample of
blood and liver tissue. Plasma
AST, ALT, ALP, MDA, CAT and Nitrite levels; liver tissue Nitrite and Nitrate
levels were increased (P<0.001); plasma GSH (P<0.001), SOD (P<0.001)
and GPx levels (P<0.05); liver tissue GSH, CAT, and SOD levels (P<0.001) were decreased significantly in paracetamol group
compared with control group. Paracetamol in rats formed
hepatotoxicity Taraxacum officinale Wig.
aerial part made ​​from ethanol as extract is used TOE 100 mg/kg of the amount
of hepatotoxicity significantly decreased and prevented lipid peroxidation.
Toxic effect was determined in animals administered TOE 250 mg/kg. Even if the obtained
extract had positive effects on hepatotoxicity and biochemical parameters in
liver and plasma, it was concluded that it was needed to for repair the damage
of the liver.

Keywords

Antioxidant, Extract, Lipid peroxidation, Paracetamol, Taraxacum officinale

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