Koç Spermasının Dondurulmasında Bireysel Farklılıkların Etkisi

Year 2017, , 79 - 83, 30.06.2017

Çiğdem Çebi Şen , Recai Kulaksız Ali Daşkın Ergun Akçay

https://doi.org/10.31196/huvfd.325767

Abstract

Bu çalışma, koç spermasının dondurulmasında
bireysel farklılıkları ortaya koymak ve bu farklılık üzerine sulandırıcı etkisini
belirlemek amacıyla yapıldı. Ejakulatlar 5 Karayaka koçundan sezon içi
dönemde suni vajina yardımıyla haftada bir kez alındı. Sperma örnekleri bireysel olarak 4 farklı sperma sulandırıcısı (Yağsız süt tozu, Tris, Andromed, Bioxcell) ile
sulandırıldı. Sulandırılmış sperma
örneklerini içeren payetler +4 °C’de 2 saat süreyle ekilibre edildikten sonra sıvı azot
buharında (-120 °C) 15 dakika süreyle donduruldu ve sıvı azot (-196 °C) içinde saklandı. Tüm gruplardaki payetler 37 °C’de 30 saniye süreyle çözdürüldü. Her bir koça
ait çözdürülmüş sperma örneklerinde spermatozoa motilitesi (%), canlı spermatozoa
(%) ve anormal spermatozoa (%) yönünden değerlendirildi.
Yağsız süt tozu sulandırıcısı ile dondurulan
sperma örneklerinde çözüm sonu daha yüksek motilite elde edilirken, en düşük
motilite oranı Bioxcell sulandırıcısından elde edilmiştir. Sonuç olarak, sunulan çalışmada elde edilen in vitro sperma kalitesi
bulgularına göre yağsız süt tozu sulandırıcısının diğer sulandırıcılardan daha
üstün olduğu ve koçlar arası bireysel farkı azalttığı
bulunmuştur.

Keywords

Bireysellik, Koç, Sperma, Sulandırıcılar.about:blankr: Bireysellik, Sulandırıcılar

Response to Different Semen Extenders of Individual Ram Semen

Abstract

This
study was conducted to evaluate individual differences in freezing ram sperm
and to determine the extenders and individual sperm interaction. The ejaculates were collected from 5 Karayaka rams by artificial
vagina twice a week during the breeding season. The each ejaculate was split
into 4 equal aliquots and diluted with skimmed milk, Tris, Andromed and
Bioxcell extenders. Straws contained semen was equilibrated at +4 °C for 2 h and than frozen in vapor of (15 min at -120 °C) liquid nitrogen and stored in liquid nitrogen. Frozen semen was
thawed in a water bath at 37 °C for 30 seconds. In thawed semen, percentages of spermatozoa
motility, viability, and abnormal spermatozoa, for each ejaculate were
determined. The post-thaw sperm motility was significantly higher in skimmed
milk extender post-thaw, while lowest motility rate was obtained from Bioxcell
diluent. Consequently, it was found that skimmed milk extender was better than
the other extenders and decreased individual differences between rams.

Keywords

Extenders, Individuality, Ram, Semen

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