Teke Spermasının +4 ⁰C’de Payet, Ependorf ve Falkon Tüplerinde Saklanmasının Spermatolojik Parametreler Üzerine Etkisi

Abstract

Bu çalışmanın amacı, Honamlı teke spermasının kısa süreli saklanmasında farklı büyüklükteki payet ve deney tüplerinin spermatolojik parametrelere etkisini araştırmaktır. Araştırmada beş baş Honamlı tekesi kullanıldı. Tekelerden haftada iki kez suni vagina yardımıyla üreme sezonu içinde sperma alındı. Her bir tekeden alınan nativ ejakülatlar birleştirilerek tris yumurta sarısı sulandırıcısı ile sulandırıldı ve payet (0.25; 0.5 ml), Ependorf tüpü (0.5; 1.5 ml), Falkon tüpü (15; 50 ml) olarak 6 farklı grup oluşturuldu ve +4 ⁰C’de saklandı. Gruplar oluşturulduktan sonra 24 saat aralıklarla spermatolojik parametreler 96. saate kadar sperma motilitesi (%), morfolojik bütünlük (%), membran bütünlüğü (HOS test, %) yönünden incelendi. Morfolojik bütünlük yönünden gruplar arasında istatistiki olarak farklılık bulunmazken (P>0.05); 96. saatin sonunda en yüksek motilite (%57.50±2.50) ve membran bütünlüğü (%56.00±3.73) 0.5 ml’lik payet grubunda tespit edildi (P<0.05). Sonuç olarak  +4 ⁰C’deki saklama koşullarında motilite ve membran bütünlüğü açısından en iyi sonuç 0.5 ml’lik payet grubunda tespit edildi.

Keywords

• Teke sperması,Kısa süreli saklama,Payet,Ependorf tüpü,Falkon tüpü

Effect of Straw, Eppendorf and Falcon Tubes on Buck Semen Spermatological Parmeters at +4 ⁰C Storage

Abstract

The purpose of this study was to investigate the effect of different sized straws and test tubes on spermatological parameters during the short-term storage of the Honamli buck semen. In this study, five Honamli bucks were used. Semen was taken twice a week with artificial vagina during the breeding season. The native ejaculates collected from each individual were mixed and diluted with tris egg yolk diluent. The diluted sperm samples were filled into straws (0.25; 0.5 ml), Eppendorf tubes (0.5; 1.5 ml) and Falcon tubes (15; 50 ml) so as to form 6 different groups. stored at +4 ⁰C. Sperm motility (%), morphologic integrity (%), membrane integrity (HOS test, %) were examined at 24 hour intervals until 96. hour. Although there were no statistically significant differences between the groups on morphological integrity (P>0.05); the highest motility (57.50±2.50%) and membrane integrity (56.00±3.73%) were detected in 0.50 ml straw group (P<0.05). As a result, in terms of motility and membrane integrity the best results were determined in 0.5 ml straw group under storage conditions at +4 ⁰C.

Keywords

• Buck semen,Liquid storage,Straw,Eppendorf tube,Falcon tube

References

1. Alçay S, Gökçe E, Toker MB, Önder NT, Üstüner B, Uzabacı E, Gül Z, Çavuş S, 2016: Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa. Criyobiology, 72, 269-273.
2. Anderson J, 2001: The Semen of Animal and Its Use for Atificial Insemination. 1st ed., Green World Publishers, Lucknow.
3. Ansari MS, Rakha BA, Andrabi SMH, Akhter S, 2011: Effect of straw size and thawing time on quality of cryopreserved buffalo (Bubalus bubalis) semen. Reproductive biology, 11, 49-54.
4. Avdatek F, Yeni D, Gündoğan M, 2018. Merinos koçlarda spermaya katılan antioksidanların kısa süreli saklama sırasında spermatolojik parametreler ve DNA hasarı üzerine etkileri. Kocatepe Vet J, 11, 126-133.
5. Berger B, Fischerleitner F, 1992: On deep freezing of boar semen: investigations on the effects of different straw volumes, methods of freezing and thawing extenders. Reprod Dom Anim, 27, 266–270.
6. Birler S, Pabuccuoğlu S, Atalla H, Alkan S, Özdaş ÖB, Bacınoğlu S, Cirit Ü, Zavar İ, Sönmez MEC, İleri İK, 2002: Transfer of in vitro produced sheep embryos. Türk J Vet Anim Sci, 26, 1421-1426.
7. Buranaamnuay K, Tummaruk P, Singlor J, Rodriguez-Martinez H, Techakumphu M, 2009: Effects of straw volume and Equex-STM® on boar semen quality after cryopreservation. Reprod Dom Anim. 44, 69-73.
8. Christensen JM, Tiersch TR, 1997: Cryopreservation of channel cat fish spermatozoa: Effect of cryoprotectant, straw size, and formulation of extender. Thenogenology, 47, 639-645.

9. Eriksson BM, Rodriguez-Martinez H, 2000: Effect of freezing and thawing rates on the post-thaw viability of boar spermatozoa frozen in FlatPacks and Maxi-straws. Anim Reprod Sci, 63, 205–220.
10. Jamieson GM, 1991: Fish Evolution and Systematics: Evidence From Spermatozoa. Cambridge University Press, New York.
11. Johnson MS, Senger PL, Allen CH, Hancock DD, Alexander BM, Sasser RG, 1995: Fertility of bull semen pacaged in 0.25 and 0.5 mililiters French Straws. Journal of Animal Science, 73, 1914-1919.
12. Joshi A, Bag S, Mittal JP 2000: Freezability of ram spermatozoa packaged in mini and medium size straw. Indian J Anim Prod, 32, 25–26.
13. Kulaksız R, 2009: Farklı antioksidanlar eklenmiş sulandırıcılarla dondurulmuş Saanen teke spermasının in vitro değerlendirilmesi. Doktora tezi, Ankara Üniversitesi Sağlık Bilimleri Enstitüsü, Ankara.
14. Kulaksız R, Daşkın A, 2009: Farklı antioksidanlarla dondurulan Saanen teke spermasının in vitro ve in vivo değerlendirilmesi. Ankara Üniv Vet Fak Derg, 56, 201-205.
15. Lahnsteiner F, 1997: Methanol as cryoprotectant and the suitability of 1.2 ml and 5 ml straws for cryopreservation of semen from salmonid fishes. Aquaculture Research, 28, 471-479.
16. Magnus PK, Anand LF, 2010: Effect of air space in storage vials on motility of spermatozoa in chilled buck semen. Veterinary World, 3, 422-423.
17. Maxwell WMC, Watson PF, 1996: Recent progress in the preservation of ram semen. Anim Reprod Sci, 42, 55–65.
18. Paulenz H, Söderquist L, Andoy T, Nordstoga A, Gulbrandsen B, Berg KA, 2004: Fertility results after different thawing procedures for ram semen frozen in minitubes and mini straws. Theriogenology, 61, 1719-1727.
19. Sadhan B, Anil J, Naqvi SMK, Mittal JP, 2004: Effect of post-thaw incubation on sperm kinematics and acrosomal integrity of ram spermatozoa cryopreserved in medium-sized French straws. Theriogenology, 62, 415–424.
20. Schafer S, Holzmann A, 2000: The use of transmigration and spermac stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci, 59, 201–211.
21. Senger PL, Mitchell JR, Almquist JO, 1983: Influence of cooling rates and extenders upon post-thaw viability of bovine spermatozoa packaged in 0.25- and 0.5-ml French straws. J Anim Sci, 56, 1261-1268.
22. Simmet C, 1993: Kältephysikalische aspekte der gefrierkonservierung von ebersperma in ihrer auswirkung auf samenqualität und befruchtungsrate. Thesis, Hannover Veterinary College, Hannover.
23. Verna A, Kanwar KC, 1999: Effect of vitamin E on human sperm motility and lipid peroxidation in vitro. Asian J.Androl, 1, 151-154.
24. Weitze KF, Rath D, Baron G, 1987: Deep freezing of boar semen in plastic straws (in German). Deustche Tierärtzliche Wochenschrift, 94, 485-488.