Ratlarda Sisplatin Kaynaklı Nefrotoksisite Üzerine Naringeninin Koruyucu Etkisinin İncelenmesi

Year 2018, , 119 - 125, 04.07.2018

İsmail Koyuncu

https://doi.org/10.31196/huvfd.470988

Abstract

Naringenin,
insan sağlığı üzerinde biyoaktif bir etkiye sahip olup, greyfurtta baskın
bulunan doğal bir flavonondur. Bu çalışmada ratlarda sisplatin ile oluşturulan
nefrotoksisite üzerine naringenin’in böbrek dokusundaki bazı biyokimyasal
parametreler üzerine etkileri araştırıldı. Bu çalışmada, 35 adet 2 aylık wistar
albino ratlar kullanıldı. Ratlar rastgele her grupta 7 rat olacak şekilde 5
gruba ayrıldı. 1.grup (Kontrol) %1’lik DMSO i.p, 2.grup  (Cis), tek doz sisplatin., 7 mg/kg / i.p,
3.grup (NG₂₀) naringenin, 20 mg/kg/10 gün /i.p, 4.grup, (Cis+NG₂₀
) tekdoz sisplatin 7 mg/kg/ i.p + 20 mg/kg/10 gün./i.p naringenin, 5.grup
(Cis+NG₄₀) tek doz sisplatin 7 mg/kg/ i.p + 40 mg/kg/10 gün./i.p
naringenin  on gün boyunca uygulandı.
Çalışma sonunda ratlardan alınan böbrek dokusundan biyokimyasal analizler
yapıldı. Sisplatin grubunda böbrek TOS, OSI, MDA, AOPP, 8-OHdG ve NRF-2
düzeyleri kontrol grubuna göre artarken (P<0.05), böbrek TAS ve GSH
(P<0.05) düzeyleri anlamlı olarak azaldı. Sisplatinin ratlarda oluşturduğu
nefrotoksisiteyi, naringenin’in anlamlı olarak azalttığından dolayı, sisplatin'e
bağlı nefrotoksisitenin naringenin ile kontrol edilebileceği sonucuna
varılmıştır.

Keywords

Naringenin, DNA hasarı, NRF-2, TAS, TOS

Investigation of the Protective Effect of Naringenin on the Cisplatin-Induced Nephrotoxicity in Rats

Abstract

Naringenin
which have a bioactive effect on human health is a natural flavonone predominantly
found in grapefruit. In this study, the effects
of naringenin on some biochemical parameters in renal tissue in
cisplatin-induced nephrotoxicity case were investigated in rats. In this study 35 Wistar albino rats, aged 2
months were used. Rats were randomly divided into 5 groups each consisted of 7
rats,; 1.group (control) 1 % DMSO i.p, 2.group 
(Cis), one dose cisplatin., 7 mg/kg/ i.p, 3.group (NG20) naringenin, 20
mg/kg/ day /i.p, 4.group, (Cis+NG₂₀ ) one dose cisplatin 7 mg/kg/
i.p + 20 mg/kg/day./i.p naringenin, 5.group (Cis+NG₄₀) one dose cisplatin
7 mg/kg/ i.p + 40 mg/kg/day./i.p naringenin were administered for ten days. At
the end of the study biochemical analyses were made on samples of kidney tissues.
Kidney tissue levels of TOS, OSI, MDA, AOPP, 8-OHdG ve NRF-2 were increased
(P<0.05) and TAS and GSH levels (P<0.05) were decreased significantly in
cisplatin group (2. group) compared with control group. As naringenin
significantly reduced nephrotoxicity in cisplatin-induced rats and nephrotoxicity
could be controlled with naringenin.

Keywords

Naringenin, DNA damage, NRF-2, TAS, TOS

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