Ratlarda Gebeliğin Değişik Dönemlerinde Trofoblastların Özellikleri Üzerinde Histokimyasal ve İmmunohistokimyasal Çalışmalar

Year 2018, , 1 - 11, 18.12.2018

İsmail Şah Harem Belma Alabay

https://doi.org/10.31196/huvfd.501388

Cited By: 1

Abstract

Bu araştırmada, ratlarda
gebeliğin değişik dönemlerinde plasentada bulunan trofoblastlar ve plasentaya
ait diğer yapıların (desidua hücreleri, dev hücreler, glikojen hücreleri)
histokimyasal ve immunohistokimyasal olarak incelenmesi amaçlandı. Materyal
olarak gebeliğin değişik dönemlerinde bulunan, erişkin ve sağlıklı 20 adet rat
kullanıldı. Histokimyasal olarak yapılan incelemeler sonucunda, gebeliğin ilk
yarımında sitotrofoblastlar ve dev hücrelerinde kuvvetli PAS (+),
sinsisyotrofoblastlarda ise kuvvetli AB (+) reaksiyon gözlendi. Gebeliğin
ikinci yarımında görülen glikojen hücrelerinde mor renkli granüller halinde
PAS/AB (+) boyanma gözlenirken, küçük bazofilik hücreler gittikçe artan
miktarda AB (+) reaksiyon gösterdiler. Gebeliğin ilerleyen dönemlerinde
vakuollü yapı gösteren desidua hücrelerinde de artan miktarda PAS (+) reaksiyon
saptandı. İmmunohistokimyasal incelemelerde, trofoblast ve dev hücrelerinde
implantasyonun olmasından gebeliğin sonuna kadar, hormonal aktivite oldukça
fazla görüldü. Östrogen ve progesteron hormonlarına ait reseptörler hücrelerin
sitoplazma ve çekirdeklerinde yoğun olarak bulunuyordu. Gebeliğin sonuna doğru
progesteron reseptörlerinde azalmanın olduğu, fakat gebeliğin her döneminde iki
hormona ait reseptörlerde, reaksiyon şiddetinin fazla olduğu dikkati çekti.

Keywords

Histokimya, İmmunohistokimya, Plasenta, Rat, Trofoblast

Histochemical and Immunohistochemical Investigation on Trophoblasts Characteristic in Different Stages of Pregnancy in Rats

Abstract

In this study, it was aimed to examine trophoblast cells and other
placental structures (decidua cells, giant cell, glycogen cell) histochemically
and immunohistochemically in various periods of gestation in rats. Twenty
adult, healty and pregnant rats were used as materials. By histochemical
examinations it was observed that there were significant AB (+) reactions in
syncytiotrophoblasts, PAS (+) reactions in giant cells and cytotrophoblasts in
the first half of gestation. In the second half of gestation, glycogen cells were
stained with PAS/AB (+) in purple coloured granules and small basophilic cells
showed gradually increasing AB (+) reactions. In the advanced state of
gestations, decidual cells having vacuoles showed increasing PAS (+) reactions.
In immunohistochemical examinations, hormonal activities increased significantly
in trophoblasts and giant cells during the period staining from the
implantation to the end of gestation. Oestrogen and progesterone receptors were
found in high density in the cytoplasm and nucleus of the cells. Progesterone
receptors decreased slightly towards the end of pregnancy, however, reactions
were high in receptors belonging to both hormones in every period of pregnancy.

Keywords

Histochemistry, Immunohistochemistry Placenta, Rat, Trophoblast

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