Ratlarda Gebeliğin Değişik Dönemlerinde Trofoblastların Özellikleri Üzerinde Histokimyasal ve İmmunohistokimyasal Çalışmalar

Abstract

Bu araştırmada, ratlarda gebeliğin değişik dönemlerinde plasentada bulunan trofoblastlar ve plasentaya ait diğer yapıların (desidua hücreleri, dev hücreler, glikojen hücreleri) histokimyasal ve immunohistokimyasal olarak incelenmesi amaçlandı. Materyal olarak gebeliğin değişik dönemlerinde bulunan, erişkin ve sağlıklı 20 adet rat kullanıldı. Histokimyasal olarak yapılan incelemeler sonucunda, gebeliğin ilk yarımında sitotrofoblastlar ve dev hücrelerinde kuvvetli PAS (+), sinsisyotrofoblastlarda ise kuvvetli AB (+) reaksiyon gözlendi. Gebeliğin ikinci yarımında görülen glikojen hücrelerinde mor renkli granüller halinde PAS/AB (+) boyanma gözlenirken, küçük bazofilik hücreler gittikçe artan miktarda AB (+) reaksiyon gösterdiler. Gebeliğin ilerleyen dönemlerinde vakuollü yapı gösteren desidua hücrelerinde de artan miktarda PAS (+) reaksiyon saptandı. İmmunohistokimyasal incelemelerde, trofoblast ve dev hücrelerinde implantasyonun olmasından gebeliğin sonuna kadar, hormonal aktivite oldukça fazla görüldü. Östrogen ve progesteron hormonlarına ait reseptörler hücrelerin sitoplazma ve çekirdeklerinde yoğun olarak bulunuyordu. Gebeliğin sonuna doğru progesteron reseptörlerinde azalmanın olduğu, fakat gebeliğin her döneminde iki hormona ait reseptörlerde, reaksiyon şiddetinin fazla olduğu dikkati çekti.

Keywords

• Histokimya,İmmunohistokimya,Plasenta,Rat,Trofoblast

Histochemical and Immunohistochemical Investigation on Trophoblasts Characteristic in Different Stages of Pregnancy in Rats

Abstract

In this study, it was aimed to examine trophoblast cells and other placental structures (decidua cells, giant cell, glycogen cell) histochemically and immunohistochemically in various periods of gestation in rats. Twenty adult, healty and pregnant rats were used as materials. By histochemical examinations it was observed that there were significant AB (+) reactions in syncytiotrophoblasts, PAS (+) reactions in giant cells and cytotrophoblasts in the first half of gestation. In the second half of gestation, glycogen cells were stained with PAS/AB (+) in purple coloured granules and small basophilic cells showed gradually increasing AB (+) reactions. In the advanced state of gestations, decidual cells having vacuoles showed increasing PAS (+) reactions. In immunohistochemical examinations, hormonal activities increased significantly in trophoblasts and giant cells during the period staining from the implantation to the end of gestation. Oestrogen and progesterone receptors were found in high density in the cytoplasm and nucleus of the cells. Progesterone receptors decreased slightly towards the end of pregnancy, however, reactions were high in receptors belonging to both hormones in every period of pregnancy.

Keywords

• Histochemistry,Immunohistochemistry Placenta,Rat,Trophoblast

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