Deneysel Diyabetik Ratlarda Ardıç (Juniperus communis L.) Yağının Nefropati Üzerine Etkileri

Year 2018, , 192 - 199, 27.12.2018

Turan Yaman Ahmet Ufuk Kömüroğlu

https://doi.org/10.31196/huvfd.508981

Cited By: 2

Abstract

Hiperglisemi
aracılı oksidatif stres diyabetik nefropatinin (DN) patogenezinde önemli bir
rol oynar. Bu çalışmada, streptozotosin (STZ) ile indüklenmiş diyabetik
ratlarda ardıç (Juniper berry; JB) yağının renoprotektif etkisinin
araştırılması amaçlandı. Bu amaçla, 40 adet erkek Wistar albino rat rastgele seçilerek;
kontrol, diyabetes mellitus (DM), DM+akarboz, DM+ardıç yağı ve ardıç yağı olmak
üzere beş gruba ayrıldı. Deneysel diyabet, tek dozluk (55 mg/kg, periton içi
[i.p]) STZ enjeksiyonu ile oluşturuldu. DM+ardıç ve ardıç grubu ratların
yemlerine 50 ml/kg JB yağı katılarak verildi. 28 günlük deneme süresi sonunda
ratlar sakrifiye edilerek kan ve doku örnekleri alındı. Böbrek dokusunda
histopatolojik değişiklikler, immunohistokimyasal olarak caspase-3 (Kaspaz-3) ekspresyonu,
biyokimyasal olarak malondialdehit
(MDA)  ve glutasyon (GSH) konsantrasyonları ve
katalaz aktiviteleri çalışıldı. Serumda ise üre ve kreatinin düzeyleri incelendi. DM grubu
ratlarda, histopatolojik olarak tubulus epitel hücrelerinde dejenerasyon ve
nekroz, glomerular yapıda bozulma gözlemlenirken, immunohistokimyasal olarak
tubul epitel hücrelerinde caspase-3 immun reaktivitesi yüksek saptandı.
Biyokimyasal olarak DM grubu ratların böbrek dokusunda, MDA konsantrasyonu
kontrol grubuna göre anlamlı olarak yüksek ve GSH konsantrasyonu ve katalaz
aktivitesi anlamlı olarak düşük bulundu. Ayrıca DM grubunda serum üre ve kreatinin konsantrasyonları
kontrol grubundan anlamlı olarak yüksek bulundu. DM+ardıç grubu ratlarda ise,
ardıç tedavisi sonucu histopatolojik değişikliklerin düzeldiği, caspase-3 immun
reaktivitesinin azaldığı ve biyokimyasal parametrelerin iyileştiği tespit
edildi. Sonuç olarak, ardıç yağının denysel diyabetik ratlarda böbreği koruyucu
etkileri olduğu belirlendi.

Keywords

Ardıç yağı, Diyabetik nefropati, Caspase-3, Rat

Effects of Juniperus communis L. Oil on Nephropathy in Experimental Diabetic Rats

Abstract

Hyperglycaemia-mediated oxidative stress plays an
important role in the pathogenesis of diabetic nephropathy. The present study
investigated the renoprotective effect of Juniper (Juniper berry; JB) oil in
rats with streptozotocin (STZ)-induced diabetes. For this, 40
male Wistar albino rats were divided into five group, namely, control, diabetes
mellitus (DM), DM+acarbose, DM + JB oil and JB oil groups. Experimental
diabetes was established by injecting the rats with a single dose (55 mg/kg) of
STZ intraperitoneally. The rats in the DM+ardıç oil and ardıç oil groups
received 50 ml JB oil/kg diet. After 28 days, the rats were sacrificed and
their blood and tissue samples were obtained. Histopathological changes,
caspase-3 immunoexpression, malondialdehyde MDA and glutathione (GSH) concentration and catalase activities in the
kidney tissues of the rats were examined. Moreover, serum urea and creatinine
levels were examined. Histopathologically, the rats in the DM group showed
degeneration and necrosis of tubular epithelial cells and destruction of
glomerular structures. Immunohistochemically, these rats showed increased
caspase-3 immunoreactivity in the tubular epithelial cells. Biochemically, MDA
concentration was significantly higher in the kidney tissue of the rats in the
DM group than that of the rats in the control group. GSH concentration and
catalase activities were significantly lower in the kidney tissue of the rats
in the DM group than that of the rats in the control group. Moreover, serum
urea and creatinine levels were significantly higher in the rats in the DM
group than those in the control group. The rats in the DM + JB oil group showed
an improvement in STZ-induced histopathological changes. Moreover, these rats
showed decreased caspase-3 immunoexpression in the kidney tissue and improved
biochemical parameters. Thus, these results indicate that JB oil exerts
protective effects in the kidneys of diabetic rats.

Keywords

Juniper oil, Diabetic nephropathy, Caspase-3, Rat

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