Deneysel Diyabetik Ratlarda Ardıç (Juniperus communis L.) Yağının Nefropati Üzerine Etkileri

Abstract

Hiperglisemi aracılı oksidatif stres diyabetik nefropatinin (DN) patogenezinde önemli bir rol oynar. Bu çalışmada, streptozotosin (STZ) ile indüklenmiş diyabetik ratlarda ardıç (Juniper berry; JB) yağının renoprotektif etkisinin araştırılması amaçlandı. Bu amaçla, 40 adet erkek Wistar albino rat rastgele seçilerek; kontrol, diyabetes mellitus (DM), DM+akarboz, DM+ardıç yağı ve ardıç yağı olmak üzere beş gruba ayrıldı. Deneysel diyabet, tek dozluk (55 mg/kg, periton içi [i.p]) STZ enjeksiyonu ile oluşturuldu. DM+ardıç ve ardıç grubu ratların yemlerine 50 ml/kg JB yağı katılarak verildi. 28 günlük deneme süresi sonunda ratlar sakrifiye edilerek kan ve doku örnekleri alındı. Böbrek dokusunda histopatolojik değişiklikler, immunohistokimyasal olarak caspase-3 (Kaspaz-3) ekspresyonu, biyokimyasal olarak malondialdehit (MDA)  ve glutasyon (GSH) konsantrasyonları ve katalaz aktiviteleri çalışıldı. Serumda ise üre ve kreatinin düzeyleri incelendi. DM grubu ratlarda, histopatolojik olarak tubulus epitel hücrelerinde dejenerasyon ve nekroz, glomerular yapıda bozulma gözlemlenirken, immunohistokimyasal olarak tubul epitel hücrelerinde caspase-3 immun reaktivitesi yüksek saptandı. Biyokimyasal olarak DM grubu ratların böbrek dokusunda, MDA konsantrasyonu kontrol grubuna göre anlamlı olarak yüksek ve GSH konsantrasyonu ve katalaz aktivitesi anlamlı olarak düşük bulundu. Ayrıca DM grubunda serum üre ve kreatinin konsantrasyonları kontrol grubundan anlamlı olarak yüksek bulundu. DM+ardıç grubu ratlarda ise, ardıç tedavisi sonucu histopatolojik değişikliklerin düzeldiği, caspase-3 immun reaktivitesinin azaldığı ve biyokimyasal parametrelerin iyileştiği tespit edildi. Sonuç olarak, ardıç yağının denysel diyabetik ratlarda böbreği koruyucu etkileri olduğu belirlendi.

Keywords

• Ardıç yağı,Diyabetik nefropati,Caspase-3,Rat

Effects of Juniperus communis L. Oil on Nephropathy in Experimental Diabetic Rats

Abstract

Hyperglycaemia-mediated oxidative stress plays an important role in the pathogenesis of diabetic nephropathy. The present study investigated the renoprotective effect of Juniper (Juniper berry; JB) oil in rats with streptozotocin (STZ)-induced diabetes. For this, 40 male Wistar albino rats were divided into five group, namely, control, diabetes mellitus (DM), DM+acarbose, DM + JB oil and JB oil groups. Experimental diabetes was established by injecting the rats with a single dose (55 mg/kg) of STZ intraperitoneally. The rats in the DM+ardıç oil and ardıç oil groups received 50 ml JB oil/kg diet. After 28 days, the rats were sacrificed and their blood and tissue samples were obtained. Histopathological changes, caspase-3 immunoexpression, malondialdehyde MDA and glutathione (GSH) concentration and catalase activities in the kidney tissues of the rats were examined. Moreover, serum urea and creatinine levels were examined. Histopathologically, the rats in the DM group showed degeneration and necrosis of tubular epithelial cells and destruction of glomerular structures. Immunohistochemically, these rats showed increased caspase-3 immunoreactivity in the tubular epithelial cells. Biochemically, MDA concentration was significantly higher in the kidney tissue of the rats in the DM group than that of the rats in the control group. GSH concentration and catalase activities were significantly lower in the kidney tissue of the rats in the DM group than that of the rats in the control group. Moreover, serum urea and creatinine levels were significantly higher in the rats in the DM group than those in the control group. The rats in the DM + JB oil group showed an improvement in STZ-induced histopathological changes. Moreover, these rats showed decreased caspase-3 immunoexpression in the kidney tissue and improved biochemical parameters. Thus, these results indicate that JB oil exerts protective effects in the kidneys of diabetic rats.

Keywords

• Juniper oil,Diabetic nephropathy,Caspase-3,Rat

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