Kangal Irkı Köpeklerde Fenol Kırmızısı Pamuk İpliği Testi (FKPT) Kullanılarak Fizyolojik Aköz Gözyaşı Üretim Miktarının Belirlenmesi

Abstract

Prekorneal gözyaşı filmi (PGF), lakrimal fonksiyonel ünitenin önemli parçalarından biridir. Evcil hayvanlarda aköz gözyaşı üretim miktarının ölçümünde Schirmer gözyaşı testi (SGT) ile fenol kırmızısı pamuk ipliği testi (FKPT) kullanılmaktadır. SGT’ye alternatif olarak geliştirilen FKPT’nin minimal invaziv olması, uygulama süresinin kısalığı ve daha az refleks gözyaşı stimülasyonuna neden olması gibi avantajları bulunmasına rağmen SGT’ye oranla daha az tercih edildiği bilinmektedir. Sunulan çalışmada bölgemizde yaygın bir şekilde yetiştirilen Kangal ırkı köpeklerde FKPT ile daha önce rapor edilmeyen fizyolojik aköz gözyaşı üretim miktarının belirlenmesi amaçlandı. Çalışmanın materyalini, 14’ü 1 yaş altı (7 Erkek, 7 Dişi, n=14, 28 göz), 14’ü ise 1 yaş üstü (7 Erkek, 7 Dişi, n=14, 28 göz) olmak üzere eşit sayıda 2 gruba (I. grup, II. grup) ayrılan toplam 28 adet Kangal ırkı sağlıklı köpek oluşturdu. Deneklerin her iki gözünün aköz gözyaşı üretim miktarları, lateral kantuslarına 15 saniye süreyle yerleştirilen fenol kırmızısı pamuk iplikleriyle ölçüldü. Ölçümler sonunda her iki grubun sağ ve sol gözlerinden alınan aköz gözyaşı üretim miktarı ortalamaları arasındaki fark istatistiki açıdan önemsiz bulundu (P>0.05). Ölçüm ortalaması 27.55±0.25 mm/15 sn. olarak kaydedilen I. grup ile ölçüm ortalaması 27.07±0.31 mm/15 sn. olarak saptanan II. grup arasındaki fark da önemsiz bulundu (P>0.05). Yaş ve göz yönü gibi değişkenler dikkate alınmaksızın tüm köpeklerin (n=28, 56 göz) fizyolojik aköz gözyaşı üretim miktarı ortalaması ise 27.31±0.20 mm/15 sn. olarak kaydedildi. Bu çıktılar, FKPT’nin Kangal ırkı köpeklerde aköz gözyaşı üretim miktarının ölçümünde güvenli ve hızlı sonuçlar verdiğini göstermektedir. Elde edilen verilerin bu alanda çalışan araştırmacılar ile klinisyenler tarafından referans olarak alınabileceği düşünülmektedir.

Keywords

• Prekorneal gözyaşı filmi,Fizyolojik aköz gözyaşı üretim miktarı,Fenol kırmızısı pamuk ipliği testi,Kangal ırkı köpek

Determination of the Physiological Aqueous Tear Production Rate by Using Phenol Red Thread Test (PRTT) in Kangal Breed Dogs

Abstract

Precorneal tear film (PTF) is an important part of the lacrimal functional unit. Schirmer tear test (STT) and phenol red thread test (PRTT) are used for the measurement of aqueous tear production rate in domestic animals. Although PRTT which has been developed as an alternative to STT, has advantages such as minimally invasive, shorter application time and less reflex tear stimulation, it is less preferred than STT. In the present study, it was aimed to determine the physiological aqueous tear production rate that has not been previously reported with PRTT in Kangal breed dogs, grown commonly in our region. The material of the study was consisted of a total of 28 Kangal breed healthy dogs, allocated to two equal groups (group I and group II), 14 lower (7 male, 7 female, n = 14, 28 eyes) and 14 higher (7 male, 7 female, n = 14, 28 eyes) than 1 years old. The aqueous tear production rate of both eyes of the subjects was measured with phenol red thread, placed in the lateral cantus for 15 seconds. The average of physiological aqueous tear production rate of the all dogs, regardless of age and eye side, was recorded as 27.31±0.20 mm/15 sec. The difference between the average of the aqueous tear production rate taken from the right and left eyes of both groups was not statistically significant (P>0.05). The difference between the mean values of group I (27.55±0.25 mm/15 sec.) and group II (27.07±0.31 mm/15 sec.) was also insignificant (P>0.05). These results show that PRTT provides safe and rapid results in the measurement of aqueous tear production rate in Kangal breed dogs. It is thought that the obtained data can be taken as reference by researchers working in this field and clinicians.

Keywords

• Precorneal tear film,Physiological aqueous tear production rate,Phenol red thread test,Kangal breed dog

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