Türkiye’de Yetiştirilen Et Irkı Kültür Sığırlarında Leptin, Ghrelin ve İnsülin Benzeri Büyüme Faktörü -1 (IGF-1) Gen Polimorfizmlerinin Belirlenmesi

Year 2019, , 108 - 115, 01.07.2019

Aydın Daş Tekin Şahin Ömer Akbulut A. Şükrü Bengü Faruk Bozkaya

https://doi.org/10.31196/huvfd.592643

Cited By: 2

Abstract

Bu
çalışma, Türkiye’de yetiştirilen et ırkı kültür sığırlarında leptin, ghrelin ve
insülin benzeri büyüme faktörü -1 (IGF-1) geni polimorfizmlerinin belirlenmesi,
polimorfizmler yönünden genotip ve allel sıklıklarının tespit edilmesi amacıyla
yapılmıştır. Çalışmanın materyalini Şanlıurfa Harranova Besi ve Tarım
İşletmesi’nde bulunan ve fenotipik değerlendirmeyle seçilmiş Hereford (n=112),
Angus (n=145), Şarole (n=54), 36 Siyah Hereford (n=36), Brahman (n=24) ve
Limousin (n=34) ırkı toplam 405 baş erkek hayvan oluşturmuştur. Hayvanların et
örneklerinden DNA izole edildikten sonra leptin, ghrelin ve IGF-1 gen
polimorfizmleri PCR-RFLP yöntemiyle belirlenmiştir. Sonuç olarak incelenen besi
sığırı sürüsünde IGF-1/SnaBI
polimorfizmi yönünden BB, AB ve AA genotiplerinin her üçü de gözlemlenmiştir.
IGF-1 lokusunda sıklığı en yüksek genotip AB, en düşük genotip ise AA şeklinde
bulunmuş olup B allelinin sıklığı (0.600) A alleline göre (0.400) yüksek olduğu
gözlenmiştir. Leptin/PstI polimorfizmi
yönünden bakıldığında da yine olası üç genotip gözlenmiştir. Bu lokusta sıklığı
en yüksek genotip CT, en düşük genotip ise TT olarak bulunmuş, C allelinin
sıklığı 0.571 bulunurken T allelinin sıklığı 0.429 olarak tespit edilmiştir.
Ghrelin/BfaI polimorfizmi yönünden
ise incelenen materyalde AA ve AG genotipleri gözlenirken GG genotipi
gözlenmemiştir. Buna bağlı olarak A allelinin sıklığı yüksek bulunurken
(0.938), G allel sıklığı ise oldukça düşük (0.062) bulunmuştur. Sonuç olarak
incelenen besi sığırı populasyonlarının IGF-1/SnaBI ve Ghrelin/BfaI
polimorfizmleri yönünden Hardy-Weinberg dengesinde, Leptin/PstI polimorfizmi yönünden ise dengede olmadığı belirlenmiştir.

Keywords

Genetik polimorfizm, Besi sığırları, Leptin, Ghrelin, IGF-1

Supporting Institution

Bingöl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

BAP-697-176-2014

Thanks

Bu çalışma Bingöl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir (Proje No: BAP-697-176-2014).

Determination of Genetic Polymorphisms of Leptin, Ghreline and Insulin Like Growth Factor-1 (IGF-1) Genes in Beef Cattle Raised in Turkey

Abstract

This
study was performed to determine polymorphisms in leptin, ghrelin and insulin
like growth factor-1 (IGF-1) genes and to identify genotype and alleles
frequencies in beef cattles raised in Sanliurfa province of Turkey. A total of
405 male beef cattles, which were raised in Harranova Live stock and
Agricultural Enterprise in Şanlıurfa and selected by phenotypic evaluation,
were included in to the study. The animal material consisted of Hereford
(n=112), Angus (n=145), Charolais (n=54), Black Hereford (n=36), Brahman
(n=24)  and Limousin (n=34) cattles.
After DNA isolation from meat samples, leptin, ghrelin and insulin like growth
factor-1 (IGF-1) gene polymorphisms were determined by using PCR-RFLP method.
All three possible genotypes of BB, AB and AA were observed in IGF-1/SnaBI gene
AB and AA genotypes had the highest and the lowest frequencies, respectively.
The frequency of B allele (0.600) was higher than that of A allele
(0.400).  With respect to PstI polymorphism
at the leptin gene, three possible genotypes were also observed. In this locus;
genotypes having the highest and the lowest frequency were CT and TT
respectively and the frequency of C allele was 0.571 while frequency of T was
0.429. At the BfaI polymorphic site of Ghrelin gene in AA and AG
genotypes were observed while GG genotype was not observed. Accordingly, the
frequency of the A allele was found to be high (0.938) and the frequency of the
G allele was found to be quite low (0.062). As a result, it was determined that
the examined beef cattle populations were in the Hardy-Weinberg equilibrium in
terms for IGF-1 / SnaBI and Ghrelin / BfaI polymorphisms but not
for Leptin/PstI polymorphism. 

Keywords

Genetic polymorphisms, Beef cattle, Leptin, Ghreline, IGF-1

Project Number

BAP-697-176-2014

References

• Akyüz B, Arslan K, Bayram D, Işcan KM, 2013: Allelic frequency of kappa-casein, growth hormone and prolactin gene in Holstein, Brown Swiss and Simmental cattle breeds in Turkey. Kafkas Universitesi Veteriner Fakultesi Dergisi, 19(3), 439-444.
• Anton I, Kovács K, Holló G, Farkas V, Lehel L, Hajda Z, Zsolnai A, 2011: Effect of leptin, DGAT1 and TG gene polymorphisms on the intramuscular fat of Angus cattle in Hungary. Livestock Science, 135, 300-303.
• Buchanan FC, Fitzsimmons CJ, Van Kessel AG, Thue TD, Winkelman-Sim DC, Schmutz SM, 2002: Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. Genetics Selection Evolution, 34(1), 105-116.
• Cheong HS, Yoon D, Kim LH, Park BL, Chung ER, Lee HJ, Shin HD, 2006: Leptin polymorphisms associated with carcass traits of meat in Korean cattle. Asian Australasian Journal of Animal Sciences, 19(11), 1529.
• Colinet FG, Portetelle D, Renaville R, 2009: Short Communication: Molecular characterization of the bovine GHRL gene. Archiv Tierzucht, 52 (1), 79-84.
• Curi RA, De Oliveira HN, Silveira AC, Lopes CR, 2005: Association between IGF-I, IGF-IR and GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livestock Production Science, 94(3), 159-167.
• Curi RA, Krauskopf MM, Hadlich JC, Fortes MRS, Vankan DM, Silva JAI, Mota MDSD, 2012: Candidate SNPs for carcass and meat traits in Nelore animals and in their crosses with Bos taurus. Pesquisa Agropecuária Brasileira, 47(2), 294-301.
• Daughaday WH, Rotwein P, 1989: Insulin-like growth factors I and II peptide, messenger ribonucleic acid and gene structures, serum, and tissue concentrations. Endocrine Reviews, 10(1), 68-91.
• De la Rosa Reyna XF, Montoya HM, Castrellón VV, Rincón AMS, Bracamonte MP, Vera WA, 2010: Polymorphisms in the IGF1 gene and their effect on growth traits in Mexican beef cattle. Genet Mol Res, 9(2), 875-883.
• Eken M, 2010: Güney Anadolu Kırmızısı ve Doğu Anadolu Kırmızısı ırkı sığırlarda büyüme hormonu salgılatıcı hormonu ve reseptörü genlerinin polimorfizmlerinin belirlenmesi. Doktora tezi, İÜ Sağlık Bilimleri Enstitüsü, İstanbul.
• Ge W, Davis ME, Hines HC, Irvin KM, Simmen RC, 2001: Association of a genetic marker with blood serum insulin-like growth factor-I concentration and growth traits in Angus cattle. Journal of Animal Science, 79(7), 1757-1762.
• Gil FMM, de Camargo GMF, de Souza FP, Cardoso DF, Fonseca PDS, Zetouni L, Tonhati H, 2013: Polymorphisms in the ghrelin gene and their associations with milk yield and quality in water buffaloes. Journal of Dairy Science, 96(5), 3326-3331.
• Gurses M, Yuce H, 2012: Determination of kappa casein gene polymorphisms and their effects on milk composition in some native cattle breeds of Turkey. Journal of Animal and Veterinary Advances, 11(7), 1023-1027.
• Gürses M, 2010: Bazı kültür ve yerli sığır ırklarında leptin geni polimorfizmlerinin belirlenmesi ve süt verimi ile bileşimi üzerine etkileri. Doktora tezi, FÜ Sağlık Bilimleri Enstitüsü, Elazığ.
• Jeanmas A, Sopannarath P, Tumwasorn S, Loongyai W, 2014: Association of SNP marker in the IGF1 gene with carcass traits in crossbred cattle among Thai Native, Brahman and Charolais. Kasetsart J. (Nat. Sci.), 48, 605- 610.
• Johnston DJ, Herd R, Reverter A, Oddy VH, 2001: Heritability of IGF-I in beef cattle and its association with growth and carcass traits. In Proc. Association for the Advancement of Animal Breeding and Genetics, 14, 163-166.
• Kaplan S, 2010: Yerli anadolu mandalarında ve esmer sığırlarda PCR-RFLP yöntemi ile prolaktin geni polimorfizminin belirlenmesi. Yüksek Lisans Tezi, SÜ Fen Bilimleri Enstitüsü, Konya.
• Kappes SM, Keele JW, Stone RT, McGraw RA, Sonstegard TS, Smith TP, Lopez-Corrales NL, Beattie CW, 1997: A second-generation linkage map of the bovine genome. Genome Research, 7(3), 235-249.
• Kononoff PJ, Deobald HM, Stewart EL, Laycock AD, Marquess FLS, 2005: The effect of a leptin single nucleotide polymorphism on quality grade, yield grade, and carcass weight of beef cattle. Journal of Animal Science, 83(4), 927-932.
• Kowalewska-Łuczak I, Szembek M, Kulig H, 2012: Ghrelin gene polymorphism in dairy cattle. Journal of Central European Agriculture, 12(4), 737-744.
• Lusk JL, 2007: Association of single nucleotide polymorphisms in the leptin gene with body weight and backfat growth curve parameters for beef cattle. Journal of Animal Science, 85(8), 1865-1872.
• Melucci LM, Panarace M, Feula P, Villarreal EL, Grigioni G, Carduza F, Corva PM, 2012: Genetic and management factors affecting beef quality in grazing Hereford steers. Meat Science, 92(4), 768-774.
• Nkrumah JD, Li C, Basarab JB, Guercio S, Meng Y, Murdoch B, Moore SS, 2004: Association of a single nucleotide polymorphism in the bovine leptin gene with feed intake, feed efficiency, growth, feeding behavior, carcass quality and body composition. Canadian Journal of Animal Science, 84(2), 211-219.
• Nobari K, Ghazanfari S, Nassiry MR, Tahmoorespur M, Jorjani E, 2010: Relationship between leptin gene polymorphism with economical traits in Iranian Sistani and Brown Swiss Cows. Journal of Animal and Veterinary Advances, 9(22), 2807-2810.
• Öner Y, Pullu M, Akin O, Elmaci C, 2011: Bursa bölgesinde yetiştirilen İsviçre Esmeri ve Siyah Alaca ırkı sığırlarda beta laktoglobulin (β-lg) ve büyüme hormonu (bGH) gen polimorfizmlerinin HaeIII ve MspI restriksiyon enzimleri kullanılarak incelenmesi. Kafkas Universitesi Veteriner Fakultesi Dergisi, 17(3), 371-376.
• Pomp D, Zou T, Clutter AC, Barendse W, 1997: Rapid communication: mapping of leptin to bovine chromosome 4 by linkage analysis of a PCR-based polymorphism, Journal of Animal Science, 75(5), 1427.
• Rogberg-Muñoz A, Cantet RJ, Fernández ME, Lirón JP, Prando A, Birchmeier AN, Giovambattista G, 2013: Longitudinal analysis of the effects of IGF1-SnaBI genotypes on the growth curve of Angus bull calves. Livestock Science, 154(1), 55-59.
• Schenkel FS, Miller SP, Ye X, Moore SS, Nkrumah JD, Li C, Williams JL, 2005: Association of single nucleotide polymorphisms in the leptin gene with carcass and meat quality traits of beef cattle. Journal of Animal Science, 83(9), 2009-2020.
• Sherman EL, Nkrumah JD, Murdoch BM, Li C, Wang Z, Fu A, Moore SS, 2008: Polymorphisms and haplotypes in the bovine NPY, GHR, GHRL, IGF2, UCP2, and UCP3 genes and their associations with measures of growth, performance, feed efficiency and carcass merit in beef cattle. Journal of Animal Science, 86(1), 1-16.
• Siadkowska E, Zwierzchowski L, Oprzadek J, Strzalkowska N, Bagnicka E, Krzyzewski J, 2006: Effect of polymorphism in IGF-1 gene on production traits in Polish Holstein-Friesian cattle. Animal Science Papers and Reports, 24(3), 225-37.
• Taniguchi Y, Itoh T, Yamada T, Sasaki Y, 2002: Genomic structure and promoter analysis of the bovine leptin gene. IUBMB Life, 53(2), 131-135.
• Trujillo AI, Peñagaricano F, Grignola MP, Nicolini P, Casal A, Espasandín AC, Chilibroste P, 2012: Using high resolution melting analysis to identify variation of NPY, LEP and IGF-1 genes in Angus cattle. Livestock Science, 146(2), 193-198.
• Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM, 1994: Positional cloning of the mouse obese gene and its human homologue. Nature, 372, 425-432.