The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines
Abstract
This study aims to assess the pharmacodynamics of aucubin, the active ingredient in plantain (Plantago), on in vitro rat bladder and trachea tissues, and explore its cell protective effects on primary lung and kidney cell lines. The study was carried out via repeated applications of acetylcholine, atropine, verapamil and oxybutynin, alongside Ca++ in a calcium-free environment, on urinary bladder tissue, and repeated applications of acetylcholine, atropine, carbachol and mecamylamine on trachea tissue. At the same time, cell viability and catalase and superoxide dismutase activity was measured on primary cell lines. The results indicate that aucubin has a relaxant effect on urinary bladder and trachea tissues. It is conceived that aucubin acts as a cholinergic antagonist through different subreceptors (muscarinic-3 receptors). The results also indicate that aucubin has a dose-dependent effect on primary lung and kidney cell lines, with increased cell viability, reduced cytotoxicity and antioxidant effects. In conclusion, it is suggested that plantain, which is used in folk medicine, and its active ingredient aucubin might have beneficial effects on bronchoconstriction and other respiratory conditions, as well as on pulmonary and renal diseases, urinary incontinence, etc.
Supporting Institution
Scientific Research Project Fund of Sivas Cumhuriyet University
Project Number
V-055 (CÜBAP, Sivas, Turkey)
Thanks
This work is supported by the Scientific Research Project Fund of Sivas Cumhuriyet University under the project number V-055 (CÜBAP, Sivas, Turkey).
References
- Akan H, Bakır Sade Y, 2015: Kâhta (Adıyaman) Merkezi ve Narince Köyü’nün etnobotanik açıdan araştırılması. BEÜ Fen Bilim Derg, 4, 219-248.
- Atta AH, Mouneir SM, 2005: Evaluation of some medicinal plant extracts for antidiarrhoeal activity. Phytother Res, 19, 481-485.
- Baydan E, Kartal M, Yurdakök B, Erdem SA, İnce S, Ekici H, Alp H, 2014: Contractile effects of Eryngium kotschyi Boiss. on rat isolated ileum and detrussor muscle. Kafkas Univ Vet Fak Derg, 20, 779-785.
- Çelebi F, Doğan A, 2000: Bazı yerel anestezik ilaçların (lidokain, bupivakain, prilokain) ratlarda mesane kasları üzerine in vitro etkisi. Kafkas Üniv Vet Fak Derg, 6, 47-53.
- Deniz L, Serteser A, Kargıoğlu M, 2010: Uşak Üniversitesi ve yakın çevresindeki bazı bitkilerin mahalli adları ve etnobotanik özellikleri. AKÜ Fen Bilim Derg, 01, 57-72.
- Estrada-Soto S, Sánchez-Recillas A, Navarrete-Vázquez G, Castillo-Espaňa P, Villalobos-Molina R, Ibarra-Barajas M, 2012: Relaxant effects of Artemisia ludoviciana on isolated rat smooth muscle tissues. J Ethnopharmacol, 139, 513-518.
- Ezer N, Avcı K, 2004: Çerkeş (Çankırı) yöresinde kullanılan halk ilaçları. Hacet Üniv Eczacı Fakült Derg, 24, 67-80.
- Fleer H, Verspohl EJ, 2007: Antispasmodic activity of an extract from Plantago lanceolata L. and some isolated compounds. Phytomedicine, 14, 409-415.
- Freshney RI, 2010: Primary Culture. In “Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications”, Ed; Freshney RI, John Wiley&Sons, Inc., New Jersey, USA.
- Hung JY, Yang CJ, Tsai YM, Huang HW, Huang MS, 2008: Antiproliferative activity of aucubin is through cell cycle arrest and apoptosis in human non-small cell lung cancer A549 cells. Clin Exp Pharmacol Physiol, 35, 995-1001.
- Kim YM, Sim UC, Shin Y, Kwon YK, 2014: Aucubin promotes neurite outgrowth in neural stem cells and axonal regeneration in sciatic nerves. Exp Neurobiol, 23, 238-245.
- Kim MB, Kim C, Chung WS, Cho JH, Nam D, Kim SH, Ahn KS, 2015: The hydrolysed products of iridoid glycosides can enhance imatinib mesylate-induced apoptosis in human myeloid leukaemia cells. Phytother Res, 29, 434-443.
- Noureini SK, Esmaili H, 2014: Multiple mechanisms of cell death induced by chelidonine in MCF-7 breast cancer cell line. Chem Biol Interact, 223, 141-149.
- Rakotondramasy VC, Mouriès C, Cachet X, Neghra A, Mourabet ME, Tillequin F, Koch M, Deguin B, 2010: A novel series of cytotoxic iridoid glucosides derived from aucubin: Design, synthesis and structure-activity relationships. Eur J Med Chem, 45, 2314-2320.
- Rønsted N, Göbel E, Franzyk H, Jensen SR, Olsen CE, 2000: Chemotaxonomy of Plantago. Iridoid glucosides and caffeoyl phenylethanoid glycosides. Phytochemistry, 55, 337-348.
- Samuelsen AB, 2000: The traditional uses, chemical constituents and biological activities of Plantago major L. A review. J Ethnopharmacol, 71, 1-21.
- Vasconcelos TB, Ribeiro-Filho HV, Lucetti LT, Magalhães PJC, 2016: β-citronellol, an alcoholic monoterpene with inhibitory properties on the contractility of rat trachea. Braz J Med Biol Res, 49, 1-9.
- Xu W, Deng Z, Guo H, Ling P, 2012: A rapid and sensitive determination of aucubin in rat plasma by liquid chromatography-tandem mass spectrometry and its pharmacokinetic application. Biomed Chromatogr, 26, 1066-1070.
- Xue HY, Niu DY, Gao GZ, Lin QY, Jin LJ, Xu YP, 2011: Aucubin modulates Bcl-2 family proteins expression and inhibits caspases cascade in H2O2-induced PC12 cells. Mol Biol Rep, 38, 3561-3567.
- Xue HY, Gao GZ, Lin QY, Jin LJ, Xu YP, 2012: Protective effects of aucubin on H2O2‐induced apoptosis in PC12 cells. Phytother Res, 26, 369-374.
- Yıldırım E, 2005: Tavşan trakeası üzerine levamizolun etkisinin tek başına ve triklorfonla birlikte araştırılması. Ankara Üniv Vet Fak Derg, 52, 23-28.
- Yıldırım A, Ekim T, 2003: Orta Anadolu Bölgesi yabancı ot florası. Bitki Koruma Bül, 43, 1-98.
Abstract
Bu çalışmada, sinir otu (Plantago) etkin maddesi aucubinin in vitro rat idrar kesesi ve trakea dokusunda farmakodinamik ve primer akciğer ve böbrek hücre hatları üzerine hücre koruyucu etkilerinin incelenmesi amaçlandı. Çalışma, idrar kesesi dokusuna tekrarlı olarak asetilkolin, atropin, verapamil ve oksibutinin ile kalsiyumsuz ortamda Ca++ verilerek, trakea dokusuna ise tekrarlı asetilkolin, atropin, karbakol ve mekamilamin uygulamalarıyla yapıldı. Aynı zamanda, primer hücre hatları üzerinde hücre canlılığı, katalaz ve süperoksit dismutaz enzim aktivitelerinin ölçümü gerçekleştirildi. Çalışma sonucunda, aucubinin idrar kesesi ve trakea dokusunda gevşetici etkisi belirlendi. Aucubinin kolinerjik antagonist olduğu, ancak bu etkisini farklı subreseptörler (muskarinik-3 reseptörler) üzerinden gösterdiği düşünüldü. Ayrıca, artan konsantrasyonlarda (1-10 μM) primer akciğer ve böbrek hücre hatlarında hücre canlılığında kontrol grubu seviyesinde kalan istatistiksel olarak önemsiz bir artışa neden olurken, aynı hücre hatlarında 20 ve 50 μM konsantrasyonlarda hücre canlılığında önemli bir düşüşe neden olduğu belirlendi. Sonuç olarak, halk arasında tedavi amacıyla kullanılan sinir otunun ve etkin maddesi aucubinin solunum yolları daralması ve hastalıkları, akciğer ve böbrek hastalıkları, üriner inkontinans vb. durumlarda yararlı olabileceği kanaatine varıldı.
Project Number
V-055 (CÜBAP, Sivas, Turkey)
References
- Akan H, Bakır Sade Y, 2015: Kâhta (Adıyaman) Merkezi ve Narince Köyü’nün etnobotanik açıdan araştırılması. BEÜ Fen Bilim Derg, 4, 219-248.
- Atta AH, Mouneir SM, 2005: Evaluation of some medicinal plant extracts for antidiarrhoeal activity. Phytother Res, 19, 481-485.
- Baydan E, Kartal M, Yurdakök B, Erdem SA, İnce S, Ekici H, Alp H, 2014: Contractile effects of Eryngium kotschyi Boiss. on rat isolated ileum and detrussor muscle. Kafkas Univ Vet Fak Derg, 20, 779-785.
- Çelebi F, Doğan A, 2000: Bazı yerel anestezik ilaçların (lidokain, bupivakain, prilokain) ratlarda mesane kasları üzerine in vitro etkisi. Kafkas Üniv Vet Fak Derg, 6, 47-53.
- Deniz L, Serteser A, Kargıoğlu M, 2010: Uşak Üniversitesi ve yakın çevresindeki bazı bitkilerin mahalli adları ve etnobotanik özellikleri. AKÜ Fen Bilim Derg, 01, 57-72.
- Estrada-Soto S, Sánchez-Recillas A, Navarrete-Vázquez G, Castillo-Espaňa P, Villalobos-Molina R, Ibarra-Barajas M, 2012: Relaxant effects of Artemisia ludoviciana on isolated rat smooth muscle tissues. J Ethnopharmacol, 139, 513-518.
- Ezer N, Avcı K, 2004: Çerkeş (Çankırı) yöresinde kullanılan halk ilaçları. Hacet Üniv Eczacı Fakült Derg, 24, 67-80.
- Fleer H, Verspohl EJ, 2007: Antispasmodic activity of an extract from Plantago lanceolata L. and some isolated compounds. Phytomedicine, 14, 409-415.
- Freshney RI, 2010: Primary Culture. In “Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications”, Ed; Freshney RI, John Wiley&Sons, Inc., New Jersey, USA.
- Hung JY, Yang CJ, Tsai YM, Huang HW, Huang MS, 2008: Antiproliferative activity of aucubin is through cell cycle arrest and apoptosis in human non-small cell lung cancer A549 cells. Clin Exp Pharmacol Physiol, 35, 995-1001.
- Kim YM, Sim UC, Shin Y, Kwon YK, 2014: Aucubin promotes neurite outgrowth in neural stem cells and axonal regeneration in sciatic nerves. Exp Neurobiol, 23, 238-245.
- Kim MB, Kim C, Chung WS, Cho JH, Nam D, Kim SH, Ahn KS, 2015: The hydrolysed products of iridoid glycosides can enhance imatinib mesylate-induced apoptosis in human myeloid leukaemia cells. Phytother Res, 29, 434-443.
- Noureini SK, Esmaili H, 2014: Multiple mechanisms of cell death induced by chelidonine in MCF-7 breast cancer cell line. Chem Biol Interact, 223, 141-149.
- Rakotondramasy VC, Mouriès C, Cachet X, Neghra A, Mourabet ME, Tillequin F, Koch M, Deguin B, 2010: A novel series of cytotoxic iridoid glucosides derived from aucubin: Design, synthesis and structure-activity relationships. Eur J Med Chem, 45, 2314-2320.
- Rønsted N, Göbel E, Franzyk H, Jensen SR, Olsen CE, 2000: Chemotaxonomy of Plantago. Iridoid glucosides and caffeoyl phenylethanoid glycosides. Phytochemistry, 55, 337-348.
- Samuelsen AB, 2000: The traditional uses, chemical constituents and biological activities of Plantago major L. A review. J Ethnopharmacol, 71, 1-21.
- Vasconcelos TB, Ribeiro-Filho HV, Lucetti LT, Magalhães PJC, 2016: β-citronellol, an alcoholic monoterpene with inhibitory properties on the contractility of rat trachea. Braz J Med Biol Res, 49, 1-9.
- Xu W, Deng Z, Guo H, Ling P, 2012: A rapid and sensitive determination of aucubin in rat plasma by liquid chromatography-tandem mass spectrometry and its pharmacokinetic application. Biomed Chromatogr, 26, 1066-1070.
- Xue HY, Niu DY, Gao GZ, Lin QY, Jin LJ, Xu YP, 2011: Aucubin modulates Bcl-2 family proteins expression and inhibits caspases cascade in H2O2-induced PC12 cells. Mol Biol Rep, 38, 3561-3567.
- Xue HY, Gao GZ, Lin QY, Jin LJ, Xu YP, 2012: Protective effects of aucubin on H2O2‐induced apoptosis in PC12 cells. Phytother Res, 26, 369-374.
- Yıldırım E, 2005: Tavşan trakeası üzerine levamizolun etkisinin tek başına ve triklorfonla birlikte araştırılması. Ankara Üniv Vet Fak Derg, 52, 23-28.
- Yıldırım A, Ekim T, 2003: Orta Anadolu Bölgesi yabancı ot florası. Bitki Koruma Bül, 43, 1-98.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Articles |
| Authors | |
| Project Number | V-055 (CÜBAP, Sivas, Turkey) |
| Publication Date | June 25, 2020 |
| Submission Date | May 15, 2020 |
| Acceptance Date | June 18, 2020 |
| Published in Issue | Year 2020 |
