Investigation of Os Coxae in Horses Using Geometric Morphometry Method

Abstract

In contrast to normal morphometric measurements, geometric morphometry is a method that can explain the differences and variations on the x-y coordinates of the specified points on a 2D plane. In this method, two-dimensional samples, such as pictures or x-rays, are used. The study aims to investigate the right os coxae of 23 adults (12 males, 11 females) horses geometrically. Photographs were taken from each sample at 120 centimeters distance and landmarks were made on these samples. 13 determining landmarks were used on os coxae. Medial endpoint of the narrowest section of the body of ilium and medial endpoint of ischial spine points were found to be statistically significant in terms of female and male distinction (P < 0.001). It was seen that the remaining points in the ventrolateral section did not have a statistically significant difference in gender discrimination. However, the difference between the genders in the ventral endpoint of coxal tuber and midpoint of coxal tuber in the dorsolateral section was statistically significant (P < 0.001). The difference between females and males is visually indicated using the morphology program. In females, it was observed that the points in the inner region are more lateral than males. Gender analysis studies can be conducted on materials such as bone using the morphometric results obtained as a result of such modeling studies.

Keywords

• Geometric analysis
• horse anatomy
• os coxae
• veterinary anatomy

Geometrik Morfometri Yöntemi Kullanılarak Atlarda Os Coxae’larının İncelenmesi

Abstract

Geometrik morfometri, normal morfometrik ölçümlerin aksine 2D düzlemde belirtilen noktaların x-y koordinatlarındaki farklılıkları ve varyasyonları açıklayan bir yöntemdir. Resim veya röntgen gibi iki boyutlu örnekler kullanılır. Bu çalışmada erişkin 23 (12 erkek, 11 dişi) tane atın sağ os coxae’sı geometrik olarak incelenmesi amaçlanmıştır. Her örnekten 120 santimetre uzaklıktan fotoğraflar alınmış ve bu örnekler üzerinden işaretlemeler yapılmıştır. Os coxae üzerinde 13 adet belirleyici nokta kullanılmıştır. Cinsiyet ayırımı bakımından, spina ischiadica’nın orta noktası ve ilium gövdesinin en dar bölümünün medial uç noktası arasında istatistiksel olarak anlamlı bir fark bulunmuştur (P < 0.001). Ventrolateral bölümde kalan noktaların cinsiyet ayrımında istatistiksel olarak anlamlı bir fark göstermediği görülmedi. Bununla birlikte, cinsiyetler arasında tuber coxae’nın ventral son noktası ve dorsolateral bölümünün orta noktası istatistiksel olarak anlamlıydı (P < 0.001). Dişi ve erkekler arasındaki fark, morfoloji programı kullanılarak gösterildi. Dişilerde iç bölgedeki noktalar erkeklere göre daha yanal olduğu gözlemlendi. Bu modelleme çalışmaları sonucunda elde edilen morfometrik sonuçlar kullanılarak kemik gibi materyaller üzerinde cinsiyet analizi çalışmaları yapılabilmektedir.

Keywords

• Geometrik anliz
• at anatomisi
• os coxae
• veteriner anatomi

References

1. Allen MJ, 2013: Biochemical markers of bone metabolism in animals: uses and limitations. Vet Clin Pathol, 32 (3): 101‐113.
2. Álvarez A, Ercoli MD, Prevosti FJ, 2013: Locomotion in some small to medium-sized mammals: a geometric morphometric analysis of the penultimate lumbar vertebra, pelvis and hindlimbs. Zool, 116: 356-371.
3. Ashdown RR, Done SH, 2001: Color Atlas of Veterinary Anatomy, Volume 2: The Horse, 2nd ed. Mosby Elsevier, Missouri, USA.
4. Aytek AI, 2017: Geometrik Morfometri. MASROP E-Dergi, 11 (17): 1-7.
5. Bahadır A, Yıldız H, 2010: Veteriner Anatomi: Hareket Sistemi ve İç Organlar. 2nd ed. Ezgi Kitapevi, Bursa, Turkey.
6. Bernal V, 2007: Size and Shape Analysis of Human Molars: Comparing Traditional and Geometric Morphometric Techniques. Homo, 58 (4): 279-296.
7. Bignon O, Baylac M, Vigne JD, Eisenmann V, 2005: Geometric morphometrics and the population diversity of Late Glacial horses in Western Europe (Equus caballus arcelini): phylogeographic and anthropological implications. J. Archaeol. Sci., 32: 375-391.
8. Boonsri B, Pitakarnnop T, Buddhachat K, Changtor P, Nganvongpanit K, 2019: Can Feline (Felis catus) Flat and Long Bone Morphometry Predict Sex or Skull Shape? Anat. Sci. Int, 94: 245-256.

9. Bytheway JA, Ross AH, 2010: A geometric morphometric approach to sex determination of the human adult os coxa. J. Forensic Sci., 55 (4): 859-864. Cardini A, Thorington Jr RW, 2006: Postnatal ontogeny of marmot (Rodentia, Sciuridae) crania: allometric trajectories and species divergence. J. Mammal., 87 (2): 201-215.
10. Cervantes I, Baumung R, Molina A, Druml T, Gutierrez JP, Sölkner J, Valera M, 2009: Size and shape analysis of morphofunctional traits in the Spanish Arab horse. Livest. Sci., 125: 43-49.
11. Colville TP, Bassert JM, 2015: Clinical anatomy and physiology for veterinary technicians. 3rd ed. Mosby Elsevier, Missouri, USA.
12. Danilo L, Remy J, Vianey-Liaud M, Merigeaud S, Lihoreau F, 2015: Intraspecific variation of endocranial structures in extant Equus: a prelüde to endocranial studies in fossil equoids. J. Mammal. Evol., 22: 561-582.
13. Delguste C, Doucet M, Gabriel A, Guyonnet J, Lepage O, Amory H, 2011: Assessment of a bone biopsy technique for measuring tiludronate in horses: a preliminary study. Can. J. Vet. Res., 75 (2): 128‐133.
14. Desevaux C, Laverty S, Doize B, 2000: Sternal bone biopsy in standing horses. Vet Surg, 29 (4): 303‐308.
15. Drake AG, Klingenberg CP, 2010: Large-scale diversification of skull shape in domestic dogs: disparity and modularity. Am. Nat., 175 (3): 289-301.
16. Dursun N, 2006: Veteriner Anatomi 1. 10th ed., Medisan Yayınevi, Ankara, Turkey.
17. Dyce KM, Sack WO, Wensing CJG, 2016: Textbook of Veterinary Anatomy. 3rd ed., Saunders Elsevier, Pennsylvania, USA.
18. Finka LR, Luna SP, Brondani JT, Tzimiropoulos Y, McDonagh J, Fanworth MJ, Ruta M, Mills DS, 2019: Geometric Morphometrics for the Study of Facial
19. Expressions in Non-Human Animals, Using the Domestic Cat as an Exemplar. Sci. Rep. 2019; 9 (9883): 1-12.
20. Frandson RD, Wilke WL, Fails AD, 2009: Anatomy and physiology of farm animals. 7th ed., Wiley-Blackwell, Iowa, USA.
21. Franklin D, Oxnard CE, O'Higgins P, Dadour I. Sexual dimorphism in the subadult mandible: quantification using geometric morphometrics. Journal of Forensic Sciences 2007; 52 (1): 6-10.
22. Fureix C, Hausberger M, Seneque E, Morisset S, Baylac S, Cornette R, Biquand V, Deleporte P, 2011: Geometric morphometrics as a tool for improving the comparative study of behavioural postures. Naturwissenschaften, 98: 583-592.
23. Geiger M, Evin A, Sánchez-Villagra MR, Gascho D, Mainini C, Zollikofer CP, 2017: Neomorphosis and heterochrony of skull shape in dog domestication. Sci. Rep., 7 (1): 1-9.
24. Gonzalez NC, RascónPérez J, Chamero B, Cambra-Moo O, Martin AG, 2017: Geometric morphometrics reveals restrictions on the shape of the female os coxae. J. Anat., 230 (1): 66-74.
25. Hammer Ø, Harper DAT, Ryan PD, 2011: PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontol Electron, 4 (1): 1-9.
26. Kaya A, Gharakhloo MM, Coşkun Y, 2018: Geographic variation in the skull morphology of Ellobius lutescens Thomas, 1897 (Mammalia: Rodentia) by geometric morphometrics. Vertebr. Zool., 68 (2): 157-164.
27. Klingenberg CP, 2011: Morphoj: an Integrated Software Package for Geometric Morphometrics. Mol. Ecol. Resour., 11 (2): 353-357.
28. Konig HE, Liebich HG, 2004: Veterinary anatomy of domestic animals: textbook and color atlas. 6th ed., Schattauer GmbH, Stuttgart, Germany.
29. Maass P, Friedling LJ, 2019: Morphometric Analysis of the Neurocranium in an Adult South African Cadaveric Sample. J. Forensic Sci.; 64 (2): 367-374.
30. Misheff MM, Stover SM, Pool RR, 1992: Corticocancellous bone biopsy from the 12th rib of standing horses. Vet Surg, 21 (2): 133‐138.
31. Noble J, Cardini A, Flavel A, Franklin D, 2019: Geometric morphometrics on juvenile crania: Exploring age and sex variation in an Australian population. Forensic Sci. Int., 294: 57-68.
32. Ozdemir ST, Ercan I, Ozkaya G, Cankurt NS, Erdal YS, 2010: Geometric Morphometric Study and Cluster Analysis of Late Byzantine and Modern Human Crania. Coll. Antropol., 34 (2): 493-499.
33. Parés-Casanova PM, 2014: Geometric Morphometrics for the Study of Hemicoxae Sexual Dimorphism in a Local Domestic Equine Breed. J. Morphol. Sci. 31 (4): 214-218.
34. Rebsamen E, Geyer H, Fürst A, Eser MW, Koch C, Kummer M, 2014: Haematuria in two geldings caused by osteochondroma of the os pubis: Case reports and anatomic study of the os pubis in 41 cadaveric pelvises. Equine Vet. Educ., 24 (1): 30-37.
35. Rohlf FJ, 2015: tpsDig2 Version 2.18., Department of Ecology and Evolution, State University of New York at Stony Brook. New York, USA.
36. Savage CJ, Jeffcott LB, Melsen F, ØStblom LC, 1991: Bone biopsy in the horse: 1. Method using the wing of the ilium. J. Vet. Med., 38 (1-10): 776‐783.
37. Savage CJ, Tidd LC, Østblom LC, Melsen F, Jeffcott LB, 1991: Bone biopsy in the horse: 3. Normal histomorphometric data according to age and sex. J. Vet. Med., 38 (1-10): 793‐797.
38. Schutz H, Polly PD, Krieger JD, Guralnick RP, 2009: Differential sexual dimorphism: size and shape in the cranium and pelvis of grey foxes (Urocyon). Biol. J. Linn. Soc. Lond., 96: 339-353.
39. Seetah K, Cucchi T, Dobney K, Barker G. 2014: A geometric morphometric re-evaluation of the use of dental form to explore differences in horse (Equuscaballus) populations and its potential zooarchaeological application. J. Archaeol. Sci., 41: 904-910.
40. Slice DE, 2007: Geometric Morphometrics. Annu. Rev. Anthropol., 36: 261–281.
41. Steiger R, Geyer H, Provencher A, Perron-Lepage MF, Von Salis B, Lepage OM, 1999: Equine bone core biopsy: evaluation of collection sites using a new electric drilling machine. Equine Practice, 21: 14‐21.
42. Yalçın H, Kaya MA, Arslan A, 2010: Comparative geometrical morphometries on the mandibles of anatolian wild sheep (Ovis gmelini anatolica) and akkaraman sheep (Ovis aries). Kafkas Univ. Vet. Fak. Derg., 16 (1): 55-61.
43. Yalçın H, Kaya MA, 2009: Comparative geometrical morphometry on the skull bones of anatolian wild sheep and akkaraman sheep. Atatürk Üniversitesi Vet. Bil. Derg., 4 (2): 105-116.
44. Yalçın H, Kayış SA, Arslan A, 2007: A Comparative Macro-Anatomic, Mechanical and Geometric Morphometrics Study on Tree and Ground Squirrel and Rat. Vet. Bil. Derg., 23 (1):83-95.