Güvercinde (Columbidae columbiformes) Pekten Okulinin Histomorfolojik Yapısı

Year 2017, Volume: 6 Issue: 1, 90 - 94, 30.06.2017

Deniz Korkmaz

https://doi.org/10.31196/huvfd.325775

Cited By: 2

Abstract

Yapılan çalışmada
güvercinde pekten okulinin histomorfolojik olarak araştırılması ve diğer
kanatlı türleriyle farklılıklarının ortaya konulması amaçlandı. Çalışma materyalini Veteriner Fakültesi
Hayvan Hastanesine getirilen ve kurtarılamayan iki adet güvercinlerden alınan sağlıklı
göz dokusu örnekleri oluşturdu. Alınan doku örnekleri %10’luk nötral tamponlu
formalin solüsyonunda 24 saat tespit edildi. Rutin doku takip işlemlerinden
sonra bloklanan örneklerden seri kesitler alındı. Alınan bu kesitler
Crossman’ın üçlü boyama yöntemiyle boyanarak araştırma mikroskobunda incelendi.
Diğer kanatlı türlerinde olduğu gibi güvercinde de pekten okulinin nervus optikusun
retinaya giriş bölgesinden vitreusa doğru uzandığı tespit edildi. Diğer
kanatlılardan farklı olarak güvercinde pekten okulinin oturduğu kalın bazal
laminada retinanın tamamıyla kopuntuya uğramadığı, optik sinir telleri katının
bir süre daha devam ettiği görüldü. Pektenin oturduğu bazal kısımda büyük çaplı
damarlara rastlandı. Bu damarların koroidden gelen damardan köken aldığı
belirlendi. Bazalden apikale doğru gidildikçe damar çaplarının küçüldüğü tespit
edildi. Bu nedenle damarlar çaplarına göre primer, sekonder ve tersiyer
damarlar olarak sınıflandırıldı. Sınıflandırılan her damar grubundan rastgele örnekleme
yöntemiyle 20 tanesinin çapları ölçülerek, ortalama damar çapı değerleri elde
edildi. Pektenin bazal kısmında ayrıca az miktarda melanositlere de rastlandı.
Bu melanositlerin de koroidin lamina vaskulozasında bulunan melanositlerden köken
almış olabileceği düşünüldü. Pektenin apikale doğru uzanan kıvrımlarında
damarlar ve melanositlerin yanı sıra pekteneal hyalositlere de rastlandı. Yapılan çalışmada elde edilen bulgular
ile güvercin pekten okulisinde pekten damarlarının ve melanositlerin koroidden
köken aldığı ilk olarak kanıtlanmış bulunmaktadır.

Keywords

Damar, Güvercin, Histomorfoloji, Melanosit, Pekten okuli

Histomorphological Structure of Pecten Oculi in Pigeons (Columbidae columbiformes)

Abstract

It is aimed to research the histomorphological structure of pecten
oculi in pigeons and to suggest the differences between other types of avian.
Tissue samples
from healthy eyes obtained from two pigeons
came to the animal hospital were used. Tissue samples were fixed in 10%
neutral-buffered formalin solution for 24 hours. After fixation samples were
treated routine procedure and blocked. Serial sections were taken from the
blocked. Those sections were examined under the microscope after stained with by
Crossman’s triple stain. The pecten oculi projects from the optic nerve head
into the vitreous chamber. Differently, it was seen that retina in pigeons was
not completely separate from pecten base and ganglion cell layer were continued
for a bit more. There were vessels with wide diameters on the basal part where
pecten was located. This vessel was found to have originated from choroid. It
was also seen that vessel diameters got smaller as it went to the apical from
the basal. So, the vessels were classified as primer, secondary, and tertiary
vessel. Diameters of 20 randomly chosen vessels from each sections were
measured and an average diameter value was found. There was also a little
amount of melanocyte in the basal part of the pecten. These melanocytes were
based on the laminated vasculosa of the choroid. Pecten oculi were covered
pecteneal membrane. Besides pecteneal hyalocytes were found in the pecteneal
limiting membrane. According to the findings of the study, it was first proven
to have originated from choroid that pigeon’s veins and melanocytes at the
pecten oculi.

Keywords

Vessel, Pigeon, Histomorphology, Melanocyte, Pecten oculi

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