Abstract
In
terms of the future of livestock businesses, obtaining calf from cows is
crucial. There are many factors that prevent fertility. These factors include
hereditary and functional problems (an- oestrus, sub-oestrus, cysts),
infectious diseases, and miscarriage. It is noteworthy that some cows do not
become pregnant despite the numerous inseminations even in the absence of an
appearent reason. In this
study it was aimed to investigate the incidence of immunological infertility by
determining immunglobulin levels in repeat breeder cows. In the study 50 cows
which calved at least once and were not pregnant despite at least 3 subsequent
inseminations although they did not have any disorders preventing fertility as
assessed by rectal and ultrasonographic examinations, were used. Antisperm
antibodies (ASA) were detected by ELISA test in blood serum samples obtained
after insemination. As a result, ASA were detected in 14.33% of the
cows that were at least 3 times inseminated but not conceived at any time in
normal field conditions.
References
- Alexander NJ, Anderson DJ, 1989: Immunological factors in reproductive fitness. In “Reproduction in Mammals”, Ed; Austin CR, and Short RV, Cambridge Universty Press, Cambridge.
- Ahuja AK, Cheema RS, Kumar A, 2016: Status of naturally developing antisperm antibodies in serum of calves, heifers, cows and their effect on in vitro capacitation and acrosome reaction. J Bio Innov 5 (6), 874-889.
- Bradley MP, Hinds LA, Bird PH, 1997: A bait-delivered immunocontraceptive vaccine for the european red fox by the year 2002? Reprod Fert Dev, 9, 111-116.
- Doğan İ, Gökçen H, 2001: Döl tutmayan inek ve düvelerde penetrasyon testinin kullanım olanakları. J Fac Vet Med, 20, 91-98.
- Kim CA, Parrish JJ, Momont HW Lunn DP, 1999: Effects of experimentally generated bull antisperm antibodies on in vitro fertilization. Bıology of Reproduction, 60, 1285–1291.
- Minbay A, Aydın N, Akay Ö, İzgür M, Diker S, 1994: İmmunoloji, Medisan Yayınevi, Ankara, Türkiye.
- Naz RK, 1999: Vaccine for contraception targeting sperm. Immunol Rev, 171, 193-202.
- Panchal MT, Dholakia PM, Derashri HJ, Kodagali SB, 1990: Investigation on immunoinfertility in repeat breeding buffaloes. Indian J Anim Sci, 60, 1211-1212.
- Price RJ, Roberts TK, Gmm D, Boettcher B, 1984: Anticomplementary activity in human semen and its possible importance in reproduction. Amer J Reprod Immunol, 6, 92.
- Rhoades RA, Bell DR, 2017: Klinik Tıbbın Temelleri, İstanbul Tıp Kitabevleri, İstanbul, Türkiye.
- Risvanli A, Kaygusuzoglu E, 2004: Immunological infertility in domestic animals. J Turk Vet Med, 3-4, 52-55.
- Risvanli A, Aydin M, Kaygusuzoglu E, Bulut H, Apaydin AM, Bolat Y, 2005: The prevalence of antisperm antibodies in cattle in the eastern anatolian region of Turkey. Irish Vet J 49, 45-48.
- Srivastava SK, Shinde S, Singh SK, Werma MR, Singh AK, Nandi S, Srivastava S, Goswami TK, Bhure SK, Kumar H, Ghosh SK, 2016: Antisperm antibodies in repeat‐breeding cows: Frequency, detection and validation of threshold levels employing sperm immobilization, sperm agglutination and immunoperoxidase assay. Reproduction in Domestic Animals, 52, 195-202.
- Waziri MA, Fayemi OE, 2000: Seroprevalence of sperm antibodies in goats. Veterinarski Archiv, 70, 95-102.
- Wolff H, Schill WB, 1985: Antisperm antibodies in infertile and homosexual men: relationship to serological and clinical findings. Fertility and Sterility, 44, 673.
- Zral Z, Bendova J, Diblikova I, Vecova D, Kummer V, Makova J, Vulnik Z, 2002: Antisperm antibodies in blood sera of bulls and correlations with age, breed and ejaculate quality. Acta Vet Brno 71, 303-308.
Abstract
Hayvancılık
işletmelerinin geleceği açısından ineklerden yavru elde edilmesi çok önemli yer
teşkil eder. İneklerde infertilite
nedenleri çok çeşitlidir. Bunlar arasında herediter, işlevsel (anöstrus, suböstrus,
kistler), enfeksiyöz hastalıklar, bakım ve besleme bozuklukları sayılabilir. Günümüzde
yukarıda sayılan bu nedenlerin bulunmadığı durumlarda bile yapılan çok sayıda
tohumlamaya rağmen bazı ineklerde gebe kalamama olgularının görülmesi dikkat
çekicidir. Bu çalışmada infertil
ineklerde immunglobulin seviyelerine bakılarak immünolojik infertilitenin
tespiti ve immünolojik infertilitenin görülme sıklığının araştırılması
amaçlanmıştır. Çalışmada,
özel işletmelerde bulunan ve en az 3 kere tohumlandığı halde gebe kalmayan, rektal
ve ultrason ile yapılan muayenede fertiliteye engel herhangi bir bozukluğu bulunmayan,
daha önce en az bir kere sorunsuz doğum yapmış inekler kullanılmıştır.
Tohumlama sonrası elde edilen kan serumu örneklerinde ELISA testi ile antisperm
antikorları tespit edilmiştir. Sonuç
olarak, normal saha koşullarında test edilen herhangi bir rahatsızlığı
bulunmayan, en az 3 kere tohumlanmış ama gebe kalmamış ineklerin kan
serumlarında ELISA testi ile % 14,33 oranında antisperm antikorları (ASA) tespit
edilmiştir.
References
- Alexander NJ, Anderson DJ, 1989: Immunological factors in reproductive fitness. In “Reproduction in Mammals”, Ed; Austin CR, and Short RV, Cambridge Universty Press, Cambridge.
- Ahuja AK, Cheema RS, Kumar A, 2016: Status of naturally developing antisperm antibodies in serum of calves, heifers, cows and their effect on in vitro capacitation and acrosome reaction. J Bio Innov 5 (6), 874-889.
- Bradley MP, Hinds LA, Bird PH, 1997: A bait-delivered immunocontraceptive vaccine for the european red fox by the year 2002? Reprod Fert Dev, 9, 111-116.
- Doğan İ, Gökçen H, 2001: Döl tutmayan inek ve düvelerde penetrasyon testinin kullanım olanakları. J Fac Vet Med, 20, 91-98.
- Kim CA, Parrish JJ, Momont HW Lunn DP, 1999: Effects of experimentally generated bull antisperm antibodies on in vitro fertilization. Bıology of Reproduction, 60, 1285–1291.
- Minbay A, Aydın N, Akay Ö, İzgür M, Diker S, 1994: İmmunoloji, Medisan Yayınevi, Ankara, Türkiye.
- Naz RK, 1999: Vaccine for contraception targeting sperm. Immunol Rev, 171, 193-202.
- Panchal MT, Dholakia PM, Derashri HJ, Kodagali SB, 1990: Investigation on immunoinfertility in repeat breeding buffaloes. Indian J Anim Sci, 60, 1211-1212.
- Price RJ, Roberts TK, Gmm D, Boettcher B, 1984: Anticomplementary activity in human semen and its possible importance in reproduction. Amer J Reprod Immunol, 6, 92.
- Rhoades RA, Bell DR, 2017: Klinik Tıbbın Temelleri, İstanbul Tıp Kitabevleri, İstanbul, Türkiye.
- Risvanli A, Kaygusuzoglu E, 2004: Immunological infertility in domestic animals. J Turk Vet Med, 3-4, 52-55.
- Risvanli A, Aydin M, Kaygusuzoglu E, Bulut H, Apaydin AM, Bolat Y, 2005: The prevalence of antisperm antibodies in cattle in the eastern anatolian region of Turkey. Irish Vet J 49, 45-48.
- Srivastava SK, Shinde S, Singh SK, Werma MR, Singh AK, Nandi S, Srivastava S, Goswami TK, Bhure SK, Kumar H, Ghosh SK, 2016: Antisperm antibodies in repeat‐breeding cows: Frequency, detection and validation of threshold levels employing sperm immobilization, sperm agglutination and immunoperoxidase assay. Reproduction in Domestic Animals, 52, 195-202.
- Waziri MA, Fayemi OE, 2000: Seroprevalence of sperm antibodies in goats. Veterinarski Archiv, 70, 95-102.
- Wolff H, Schill WB, 1985: Antisperm antibodies in infertile and homosexual men: relationship to serological and clinical findings. Fertility and Sterility, 44, 673.
- Zral Z, Bendova J, Diblikova I, Vecova D, Kummer V, Makova J, Vulnik Z, 2002: Antisperm antibodies in blood sera of bulls and correlations with age, breed and ejaculate quality. Acta Vet Brno 71, 303-308.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research |
| Authors | |
| Publication Date | July 4, 2018 |
| Submission Date | March 5, 2018 |
| Acceptance Date | June 7, 2018 |
| Published in Issue | Year 2018 Volume: 7 Issue: 1 |
