Teke Spermasının +4 ⁰C’de Payet, Ependorf ve Falkon Tüplerinde Saklanmasının Spermatolojik Parametreler Üzerine Etkisi

Year 2018, Volume: 7 Issue: 1, 93 - 97, 04.07.2018

Muhammed Enes İnanç , Şükrü Güngör Fatıma Dinç Ayhan Ata

https://doi.org/10.31196/huvfd.470970

Abstract

Bu çalışmanın amacı,
Honamlı teke spermasının kısa süreli saklanmasında farklı büyüklükteki payet ve
deney tüplerinin spermatolojik parametrelere etkisini araştırmaktır.
Araştırmada beş baş Honamlı tekesi kullanıldı. Tekelerden haftada iki kez suni
vagina yardımıyla üreme sezonu içinde sperma alındı. Her bir tekeden alınan
nativ ejakülatlar birleştirilerek tris yumurta sarısı sulandırıcısı ile
sulandırıldı ve payet (0.25; 0.5 ml), Ependorf tüpü (0.5; 1.5 ml), Falkon tüpü
(15; 50 ml) olarak 6 farklı grup oluşturuldu ve +4 ⁰C’de saklandı. Gruplar
oluşturulduktan sonra 24 saat aralıklarla spermatolojik parametreler 96. saate
kadar sperma motilitesi (%), morfolojik bütünlük (%), membran bütünlüğü (HOS
test, %) yönünden incelendi. Morfolojik bütünlük yönünden gruplar arasında
istatistiki olarak farklılık bulunmazken (P>0.05); 96. saatin sonunda en
yüksek motilite (%57.50±2.50) ve membran bütünlüğü (%56.00±3.73) 0.5 ml’lik
payet grubunda tespit edildi (P<0.05). Sonuç olarak  +4 ⁰C’deki saklama koşullarında motilite ve
membran bütünlüğü açısından en iyi sonuç 0.5 ml’lik payet grubunda tespit
edildi.

Keywords

Teke sperması, Kısa süreli saklama, Payet, Ependorf tüpü, Falkon tüpü

Effect of Straw, Eppendorf and Falcon Tubes on Buck Semen Spermatological Parmeters at +4 ⁰C Storage

Abstract

The
purpose of this study was to investigate the effect of different sized straws
and test tubes on spermatological parameters during the short-term storage of
the Honamli buck semen. In this study, five Honamli bucks were used. Semen was
taken twice a week with artificial vagina during the breeding season. The
native ejaculates collected from each individual were mixed and diluted with
tris egg yolk diluent. The diluted sperm samples were filled into straws (0.25;
0.5 ml), Eppendorf tubes (0.5; 1.5 ml) and Falcon tubes (15; 50 ml) so as to form
6 different groups. stored at +4 ⁰C. Sperm motility (%), morphologic integrity
(%), membrane integrity (HOS test, %) were examined at 24 hour intervals until 96.
hour. Although there were no statistically significant differences between the
groups on morphological integrity (P>0.05); the highest motility (57.50±2.50%)
and membrane integrity (56.00±3.73%) were detected in 0.50 ml straw group
(P<0.05). As a result, in terms of motility and membrane integrity the best
results were determined in 0.5 ml straw group under storage conditions at +4 ⁰C.

Keywords

Buck semen, Liquid storage, Straw, Eppendorf tube, Falcon tube

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