Kilis, Halep ve Kıl Keçilerinde Beta-Kazein (CSN2) Genindeki Çeşitliliğin Allel Spesifik PCR, Real-Time PCR ve Sekans Analizi ile Araştırılması

Year 2019, Volume: 8 Issue: 1, 70 - 76, 01.07.2019

Faruk Bozkaya , Akın Yiğin Mehmet Osman Atlı

https://doi.org/10.31196/huvfd.592558

Abstract

Bu çalışmada Kilis, Halep ve Kıl keçilerinde
Beta-kazein geninin (CSN2) promotor bölgesi ile 7. eksonunun belirli
nükleotitlerindeki farklılıkların allel spesifik polimeraz zincir reaksiyonu
(AS-PCR), Real-Time PCR ve sekans analizi yöntemleriyle araştırılarak söz
konusu keçi populasyonlarının bu açıdan genetik çeşitliliğinin belirlenmesi
amaçlanmıştır. Bu amaçla toplam 246 DNA örneği kullanılmıştır. CSN2 geninin
promotor bölgesindeki g1311T>C mutasyonu AS-PCR ile, 7. eksonun 404.
pozisyonundaki C>T mutasyonunun (CSN2C alleli) varlığı ise Real-Time PCR ve
sekans analizi ile incelenmiştir. AS-PCR işlemi sonucunda CSN2 geni promotor
bölgesi 1311. nükleotitte T allelinin sıklığı bütün populasyonlarda yüksek bulunmakla
birlikte Kıl keçilerinde C allelinin sıklığı (0.0489) Halep (0.0190) ve Kilis
(0.0188) keçilerinden daha yüksek bulunmuştur. Bütün populasyonlar
değerlendirildiğinde 7. eksonunun 404. pozisyonundaki T nükleotitinin (CSN2C
alleli) sıklığının (0.824) C nükleotitinden (0.176) daha yüksek olduğu
gözlenmiştir. En yüksek CSN2C alleli sıklığı Kıl keçilerinde (0.8864) en düşük
ise Kilis keçilerinde (0.6364) gözlenmiştir. Sonuç olarak Kilis, Halep ve Kıl
keçilerinde CSN2 geninin promotor bölgesindeki g1311T>C mutasyonu ile 7.
eksonun 404. pozisyonundaki C>T mutasyonu açısından çeşitlilik gösterdiği
tespit edilmiştir. Bu çeşitliliğin keçi sütünün bileşimi ve beta kazein
içeriğini etkileyip etkilemediğinin belirlenmesi için daha fazla araştırmaya
ihtiyaç bulunmaktadır.

Keywords

Keçi, Süt, CSN2, Polimorfizm

Supporting Institution

Harran üniversitesi bilimsel araştırmalar projeleri koordinasyon birimi

Project Number

15116/2017

Thanks

Bu çalışma Harran üniversitesi bilimsel araştırmalar projeleri koordinasyon birimi tarafından desteklenmiştir (Proje No.15116/2017).

Investigation on the polymorphism of Beta-Casein gene (CSN2) in Kilis, Aleppo and Hair Goats by using allele specific PCR, Real-Time PCR and sequence analysis

Abstract

The objective of this study
was to investigate the genetic variability of Beta-casein gene (CSN2) with
respect to certain nucleotides at the promotor region and exon 7 in Kilis,
Aleppo and Hair goats by using allele specific polymerase chain reaction
(AS-PCR), real-time PCR and sequencing methods. A total of 246 DNA samples were
used. Presence of the mutation at the promotor region (g1311T>C) of CSN2
gene was searched by using AS-PCR, while the polymorphism at the 404th
nucleotide of the exon 7 was analysed using Real-Time PCR and sequence
analysis. By using AS-PCR frequency of the T allele at the 1311th
nucleotide of the promotor region was found to be higher than that of C allele,
while the frequency of the C allele was higher in Hair goats (0.0489) than that
in Aleppo (0.0190) and Kilis goats (0.0188). When all three populations were
considered, frequency of T nucleotide (CSN2C allele) at the 404th
nucleotide position of the exon 7 (0.824) was higher than that of C nucleotide
(0.176). The highest frequency of the CSN2C allele was found in Hair goats
(0.8864) while the lowest frequency of this allele was in Kilis goats (0.6364).
The results indicated that polymorphism was present with respect to g1311T>C
mutation at the promotor region and at the 404th nucleotide of the
exon 7 of the CSN2 gene among Kilis, Aleppo and Hair goats. Further studies are
required for determining the effect of these polymorphism on the composition
and beta-casein content the milk in these goat populations.

Keywords

Goat, Milk, CSN2, Polymorphism

Project Number

15116/2017

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