Bazı kaba yemlere ilave edilen probiyotiklerin in vitro organik madde sindirimi ve metan üretimi üzerine etkileri

Year 2019, Volume: 8 Issue: 1, 93 - 98, 01.07.2019

Ali Güler Oktay Kaplan Faruk Bozkaya

https://doi.org/10.31196/huvfd.592585

Abstract

Bu çalışma ruminantlarda yaygın olarak kullanılan bazı
kaba yemlere katılan probiyotiklerin (Lactobacillus
rhamnosus, Bifidobacterium lactis
ve Saccharomyces boulardii) in vitro
ortamda metan gazı oluşumuna etkisini tespit etmek için yapılmıştır. Bu amaçla
%0.1 oranında probiyotik ilave edilen öğütülmüş kaba yem örnekleri rumen sıvısı
içeren özel cam tüpler içerisinde 39 °C’de 24 saat inkube edilmiştir.
İnkubasyon sonunda oluşan toplam gaz içerisindeki metan (CH₄) gazı
ve karbondioksit (CO₂) yüzdesi CH₄ ölçüm cihazı
yardımıyla belirlenmiştir. Ayrıca her bir deneme grubundaki in vitro organik
madde sindirilebilirliği (IVOMS), amonyak azotu miktarı (NH₃-N),
metabolik enerji (ME) ve pH değerleri belirlenmiştir. Buğday samanına ilave
edilen B. lactis’in oluşan toplam
gaz, CH₄ ve CO₂ hacmini ve IVOMS’ni düşürdüğü, S. boulardii’nin ise CH₄
yüzdesini yükseltirken, CO₂ yüzdesini düşürdüğü gözlenmiştir. Çayır
kuru otuna ilave edilen L. rhamnosus
oluşan toplam gaz miktarını, CH₄ miktarını ve IVOMS’ni yükseltirken
CH₄ yüzdesini etkilememiştir. Silaj ve yonca kuru otuna ilave edilen
probiyotikler CH₄ ve CO₂ düzeylerini etkilememiştir.
Sonuç olarak çalışmada buğday samanına katılan B. lactis dışındaki probiyotik mikroorganizmalar CH₄
üretimini arttırmış ya da etkilememiştir. Buğday samanına ilave edilen B. lactis’in CH₄ miktarını
azaltması, Çayır kuru otuna ilave edilen L.
rhamnosus’un ise CH₄ miktarını arttırmasının söz konusu yemlerin
IVOMS’ni etkilemesinden kaynaklandığı, bu nedenle sunulan çalışmada kullanılan
probiyotiklerin CH₄ miktarını azaltmakta etkili olmadığı sonucuna
varılmıştır. 

Keywords

Probiyotik, Kaba yem, in vitro, Metan üretimi

Effects of Probiotics Added to Some Roughages on in vitro Organic Matter Digestibility and Methane Production

Abstract

The objective of this study
was to determine the effects of probiotics (Lactobacillus
rhamnosus, Bifidobacterium lactis and Saccharomyces
boulardii) added to some roughages on in vitro methane production. Ground
roughage samples added with 0.1% of probiotics were incubated in special glass
tubes containing rumen fluid at 39 °C for 24 h. Percentages of methane (CH₄)
and carbon dioxide (CO₂) in total gas produced were measured by
using a methane measuring device. Additionally, in vitro organic matter
digestibility (IVOMD), amount of ammonia nitrogen (NH₃-N), metabolic
energy (ME) and pH values were also determined. Addition of B. lactis to wheat straw decreased CH₄
and CO₂ amount and IVOMD while S.
boulardii increased the percentage of CH₄ and decreased the
percentage of CO₂. Among probiotics added to grass hay L. rhamnosus
increased total gas volume, CH₄ volume and IVOMD while it did not
affect the percentage of CH₄. It was detected that addition of the
probiotics to corn silage and alfa alfa roughage did not affect methane and
carbondioxyde levels. As a result, except for B. lactis, added to wheat straw, probiotic microorganisms used in
the present study increased or did not affect in vitro methane production. The
results suggested that decreased methane production in wheat straw added with B. lactis and increased methane
production in grass hay added with L. rhamnosus
resulted from the affected IVOMD of these roughages due to addition of these
probiotics and therefore use of the probiotics in this study was not effective
for reducing ruminal methane production.

Keywords

Probiotics, Roughage, in vitro, Methane production

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