Identification of Vibrio parahaemolyticus by PCR Method in Fish and Water Samples from Tigris River, Turkey

Year 2021, Volume: 10 Issue: 2, 191 - 195, 12.12.2021

Filiz Özcan Neval Berrin Arserim Aydın Ketani

https://doi.org/10.31196/huvfd.1009028

Abstract

Climate change has caused increasing Vibrio parahaemolyticus infections in areas unaffected by this pathogen. Water temperature is an important ecological factor. It is consequently of high importance to follow V. parahaemolyticus in aquatic environments and foods. The aim of this work was to detect the presence of V. parahaemolyticus in Tigris River from eight different sites namely Malabadi Bridge, Fiskaya, Ongözlü Bridge, Bağıvar, Pamukçay, Ambarçay, Seyrantepe and Silvan. Total 480 samples of water and fish were used for V. parahaemolyticus detection. Thiosulphatecitrate‐bile salt sucrose (TCBS) Agar was employed for bacteria isolation for 24 h at 37◦C. The dominant uniform bacterial colonies were purified by streaking onto the TCBS agar plates three times. Identification of this pathogen was made by PCR. A total of 480 water and fish samples were analyzed. According to the results, V. parahaemolyticus was detected in 40 (6.6%) fish samples taken from the Seyrantepe region.

Keywords

pcr, Vibrio parahaemolyticus, Fish, Water

Supporting Institution

Finansal Açıdan Destekleyen kurum bulunmamaktadır.

Project Number

Proje Numarası bulunmamaktadır.

Dicle Nehrindeki Balık ve Su Örneklerinde Vibrio parahaemolyticus’un PCR Yöntemi İle İdentifikasyonu

Abstract

İklim değişikliğinin bir sonucu olarak artan sayıda Vibrio parahaemolyticus enfeksiyonu tespit edilmiştir. Su sıcaklığı çok güçlü bir ekolojik faktördür. Dolayısıyla su ortamlarında ve su ürünlerinde V. parahaemolyticus'u takip etmek önem arzetmektedir. Bu çalışmanın amacı, Dicle nehrinde bulunan Malabadi Köprüsü, Fiskaya, Ongözlü Köprüsü, Bağıvar, Pamukçay, Ambarçay, Seyrantepe ve Silvan olmak üzere sekiz farklı yerdeki Vibrio parahaemolyticus'un varlığını araştırmaktır. Bu amaçla toplam 480 su ve balık numunesi kullanılmıştır. Bakteri izolasyonu için örnekler Tiyosülfatsitratsafra tuzu sukroz (TCBS) Agara ekim yapılmış, 37°C'de 24 saat inkübe edilmiştir ve bakteri kolonileri, TCBS agar plakalarına üç kez sürme yoluyla saflaştırılmıştır. Patojenin kesin tespiti PCR testi ile gerçekleştirilmiştir. Araştırmanın sonucunda Seyrantepe'deki 40 (6.6%) adet balık örneğinde patojenin varlığı tespit edilmiştir.

Keywords

vibrio parahaeomolyticus, Balık, PCR, Su

Project Number

Proje Numarası bulunmamaktadır.

References

• Al-Murib M, Wells S, Talke SA 2017: Estimation of Surface Water Temperature of the Tigris River System in Iraq World Environmental and Water Resources Congress.
• Babai J, Kıra R, Mohd Sınang F, Maurıce Bılung L. 2020: Occurrences of Vibrio parahaemolyticus in Retailed Freshwater Fish Trends in Undergraduate Research, 3: a1-4.
• Constantin de Magny G, Long W, Brown C, Hood R, Huq A, Murtugudde R, Colwell R 2009: Predicting the distribution of Vibrio spp. in the Chesapeake Bay: a Vibrio cholerae case study. Eco Health, 6, 378–389 .
• European Environmental Agency Climate Change,Impactsand Vulnerability in Europe Copenhagen, Denmark:European Environmental Agency, 2012. Federici S, Serrazanetti DI, Elisabetta Guerzoni M, Campana R, Ciandrini E, Baffone W, Gianott, 2018: A. Development of a rapid PCR protocol to detect Vibrio parahaemolyticus in clams, J Food Sci Technol, 55(2):749–759 .
• Froelich BA, Daines DA 2020: In hot water: Effects of climate change on Vibrio–human interactions, Environmental Microbiology, 22(10), 4101–4111.
• Guin S, Saravanan MA, Chowdhury G, Perumal Pazhani G, Ramamurthy T, Chandra SD. 2019: Pathogenic Vibrio parahaemolyticus indiarrhoeal patients, fish and aquatic environments and their potential for inter-source transmission Heliyon, 5. e01743 .
• Givens CE, Bowers JC, DePaola A, Hollibaugh JT, Jones JL 2014: Occurrence and distribution of Vibrio vulnificus and Vibrio parahaemolyticus – potential roles for fish, oyster,sediment and water. Letters in Applied Microbiology,58,503—510.
• Hemmat M, Ibrahim 1, Reham A, Amin 1, Nesreen Z, Eleiwa Hanan R, Ghanaym M. 2018: Vibrio Species in Fish and Shell Fish, Benha Veterınary Medıcal Journal,vol. 34, no. 2: 246-254 .
• Kvit A, Davis B, Jacobs J, Curriero Adjusted FC 2019: Non-Euclidean cluster detection of Vibrio parahaemolyticus in the Chesapeake Bay, USA. Geospatial Health, 14:783 .
• Logar-Henderson C, Ling R, Tuite AR, Fisman DN 2019: Effects of large-scale oceanic phenomena on noncholera noncholera vibriosis incidence in the United States: implications for climate change. Epidemiol Infect, 147 (243). Martin B, Jofre A, Garrigo M, Hugas M, Aymerich T. 2004: Quantification of Listeria monocytogenes in fermented sausages by MPN-PCR method. Letters in Applied Microbiology,39: 290-295 .
• Sanjeev S 2002: Pathogenic halophilic Vibrios in seafoods. In: Quality Assurance in Seafood Processing, 252pp. T.S. Gopala Krishna Iyer, M.K. Kandoran, Mary Thomas P.T Mathew (Eds). Central Institute of Fisheries Technology and Society of Fisheries Technologists (India), Cochi.
• Semenza JC, Trinanes J, Lohr W, Sudre B, Löfdahl M, Martinez-Urtaza J, Nichols G, Rocklöv J 2017: Environmental Suitabilityof Vibrio Infections ina Warming Climate: An Early Warning System Environmental Health Perspectives ,508.
• Terentjeva M, Eizenberga I, Novoslavskij A, Strazdiņa V, Valciņa O, Ošmjana J, Bērziņš A 2015: Bacterial microflora of freshwater fish originated from Usmas Lake in Latvia. Journal of Microbiology, Biotechnology and Food Sciences, 4 (1):74–77 .
• Türkiye Halk Sağlığı Kurumu. 2019: Mikrobiyoloji Referans Laboratuvarları Daire Başkanlığı.
• Xie T, Wu Q, Xu X, Zhang J, Guo W, Schembri M. 2015: Prevalence and population analysis of Vibrio parahaemolyticus in aquatic products from South China markets. FEMS Microbiol Lett, 362: fnv178.
• Varol M, Gökot B, Bekleyen A 2010: Assesment of Water Pollution in the Tigris River in Diyarbakır, Turkey Water Practice & Technology ,Vol 5 No 1.
• Vezzulli L, ColwellR R, Pruzzo C. 2013: Ocean warming and spread ofpathogenic vibrios intheaquaticenvironment. Microb Ecol, 65(4):81725 PMID:23280498.